Study the Behavior of Activity and Express for Enzymes

Abstract

Enzymes are be defined as soluble, colloidal, organic catalysts which are produced by living cells. Enzymes are biologic catalysts to accelerate the rate of biochemical reactions. in this study Quantification of the Enzyme Activity, Where: E = enzyme concentration; S = substrate concentration; ES = enzyme-substrate complex concentration; k1, k3= 1st order reaction rate constants (t-1); P = product concentration; k2= 2nd reaction rate constant (M-1.t-1).At first, it must be stressed that the formation of ES (enzyme-substrate complex) is an obligatory step to any type of enzyme-catalyzed reaction. The ES, depending on the reaction conditions, can form the product (forward reaction) or not (reward reaction). The immobilization technique allowed a more frequent use of biomaterials in industry, therapeutics (confection of subcutaneous capsules for controlled delivering of hormones), and lab analysis (automatic dosing equipments, enzyme electrode, biosensors and enzyme immunoassays). In industry, the use of glucose isomerase for the conversion of glucose into fructose (high fructose corn syrup), the aminoacylase for the separation of racemic mixture of amino acids, microbial lipase in triglyceride hydrolysis, and the lactase for removing lactose from milk and/or whey are stressed.