Successional Change of the Fungal Microbiome Pine Seedling Roots Inoculated With Tricholoma matsutake

Park, Ki Hyeong; Oh, Seung‐Yoon; Yoo, Shinnam; Park, Myung Soo; Fong, Jonathan J.; Lim, Young Woon

doi:10.3389/fmicb.2020.574146

Cited by 13 publications

(6 citation statements)

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“…Park and his colleagues conducted in vitro transplantation of P. densiflora seedlings inoculated with T. matsutake and showed the different results from our study covering several points [ 54 ]. Our study did not have a significant change of the transplantation which they conducted, but only observed seasonal changes and regional differences in the wild environment in which T. matsutake grows, so there was a clear difference from the in vitro experiment.…”

Section: Resultsmentioning

confidence: 87%

Pyrosequencing and Taxonomic Composition of the Fungal Community from Soil of Tricholoma matsutake in Gyeongju

Jeong

Choi

Cheon

et al. 2021

J. Microbiol. Biotechnol.

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Tricholoma matsutake, known as the pine mushroom, is an ectomycorrhizal fungus that has a symbiotic relationship with the root of Pinus densiflora trees. T. matsutake is used medicinally and is also one of the most famous food items in Northeast Asia because of its unique flavor and taste [1][2][3][4][5][6]. In South Korea, the yield of T. matsutake is changeable because environmental factors such as temperature and precipitation greatly affect its growth [7]. For this reason, studies have examined the possibility of creating a stable supply of the pine mushroom, but currently, artificial cultivation is not feasible. The effect of environmental factors (climate, soil condition, microbial community, and others) on its growth and interactions between T. matsutake and P. densiflora requires further research [8][9][10]. Soil microbial communities, in particular, are important to the mycelial growth of T. matsutake, however, few studies on the characteristics of the communities have been conducted [11][12][13][14].Mycorrhizal fungi are an important group of fungi in the soil ecosystem. They have a mutualistic relationship with living plants, exchanging nutrients and some important compounds [15][16][17][18][19]. The major group of mycorrhizal fungi is the ectomycorrhizae (ECM) fungi group. ECM fungi, including T. matsutake, considerably affect the growth of living plants by colonizing close by the rootlets of living plants. ECM fungi form ECM with roots of host plants and have advantageous symbiotic relationships with them. ECM are symbiotic organs between soil and roots and help the trees absorb water and nutrients, receiving carbohydrates from them in exchange to grow mycelium [20][21][22]. The fungus Arthrinium phaeospermum promotes mycorrhiza formation, similar to the effect of mycorrhization helper bacteria [23]. T. matsutake is an ectomycorrhizal symbiont that is important to the growth of host plant seedlings [24]. In addition, some bacteria and fungi have positive effects on T. matsutake [25]. The soil fungal community is positively influenced by plant species richness and diversity [26]. The interaction between plants and mycorrhizal fungi plays a key role in the circulation of nutrients and the balance of the ecosystem [27][28][29][30]. Therefore, the study of soil fungal communities near T. matsutake should be conducted constantly and persistently.Tricholoma matsutake is an ectomycorrhizal fungus that has a symbiotic relationship with the root of Pinus densiflora. Soil microbial communities greatly affect the growth of T. matsutake, however, few studies have examined the characteristics of these communities. In the present study, we analyzed soil fungal communities from Gyeongju and Yeongdeok using metagenomic pyrosequencing to investigate differences in fungal species diversity, richness, and taxonomic composition between the soil under T. matsutake fruiting bodies (Sample 2) and soil where the fairy ring of T. matsutake was no longer present (Sample 1). The same spot was investigated three times at interv...

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Section: Resultsmentioning

confidence: 87%

Pyrosequencing and Taxonomic Composition of the Fungal Community from Soil of Tricholoma matsutake in Gyeongju

Jeong

Choi

Cheon

et al. 2021

J. Microbiol. Biotechnol.

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“…Other studies have already reported a negative impact on Trichoderma spp. inoculations on plant microbiome diversities [72,73,74]. According to the LEfSe analysis, fungal pathogens responsible to grapevine trunk diseases, like Ilyonectria, and Diaporthe [75] reduced overtime, and/or in plants inoculated with Ta SC1.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol agents establishment and their impact on rhizosphere microbiome and induced grapevine defenses is highly soil-dependent

Leal

Eichmeier

Štůsková

et al. 2023

Preprint

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Background Without effective chemical treatments, there is an increased interest on biological control of plant diseases. Trichoderma and Bacillus species have shown biocontrol potential against several grapevine diseases. However, few studies have investigated the impact of introducing beneficial microorganisms in rhizosphere, on the indigenous soil existent microbiome. Plant-associated microbiomes are complex, and there is a limited knowledge of the mechanisms that maintain and stimulate specific plant-associated microbial communities. In this study, we tested the hypothesis that the introduction of the biological control agents (BCAs) Bacillus subtilis PTA-271 (Bs PTA-271) and Trichoderma atroviride SC1 (Ta SC1) produce distinctive modifications in the composition and co-occurrence network structure of the grapevine rhizosphere microbial community, as well as grapevine induced defenses. Results Our results demonstrated that, the fungal microbiome is more affected by factors such as soil type, BCA treatment, and sampling time than bacterial microbiome. Specifically, Ta SC1 application produced a negative impact on fungal diversity, while applications of BCAs did not affect bacterial diversity. Interestingly, the survival and establishment of both BCAs showed opposite trends depending on the soil type, indicating that the physicochemical properties of soils have a role on BCA establishment. Fungal co-occurrence networks were less complex than bacterial networks, but highly impacted by Ta SC1 application. Soils treated with Ta SC1, presented more complex and stable co-occurrence networks, with a higher number of positive correlations. Induced grapevine defenses also differed according to the soil, being more affected by BCA inoculation on sandy soil. Conclusions The findings of this research emphasize the complex relationships among microorganisms in the rhizosphere, and point to the impact of biocontrol treatments on fungal and bacterial rhizosphere microbiomes. It highlights the significance of taking into account various factors like soil type, sampling time, and BCA treatment, and their influence on the structure and dynamics of microbial communities. In the future, it is crucial to conduct additional studies to explore these microbial interactions in greater detail, with the goal of developing more precise and efficient biocontrol strategies for agriculture.

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“…Other studies have already reported a negative impact on Trichoderma spp. inoculations on plant microbiome diversities (Park et al 2020;Stummer et al 2022;Sui et al 2022). According to the LEfSe analysis, fungal pathogens responsible to grapevine trunk diseases, like Ilyonectria, and Diaporthe (Gramaje et al 2018) reduced overtime, and/or in plants inoculated with Ta SC1.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol agents establishment and their impact on rhizosphere microbiome and induced grapevine defenses is highly soil-dependent

Leal,

Eichmeier,

Štůsková

et al. 2023

Preprint

View full text Add to dashboard Cite

Without effective chemical treatments, there is an increased interest on biological control of plant diseases. Trichoderma and Bacillus species have shown biocontrol potential against several grapevine diseases. However, few studies have investigated the impact of introducing beneficial microorganisms in rhizosphere, on the indigenous soil existent microbiome. In this study, we conducted a greenhouse-based experiment to explore the effect of the two most well characterized BCAs in grapevine, Trichoderma atroviride SC1 (Ta SC1) and Bacillus subtilis PTA-271 (Bs PTA-271), on the grapevine rhizosphere bacterial and fungal microbiome, and on plant defense expression, using high-throughput amplicon sequencing, and quantitative real-time PCR, respectively. Additionally, we quantified both Ta SC1 and Bs PTA-271 in rhizosphere overtime using droplet digital PCR. Our results demonstrated that, the fungal microbiome is more affected by factors such as soil type, BCA treatment, and sampling time than bacterial microbiome. Specifically, Ta SC1 application produced a negative impact on fungal diversity, while applications of BCAs did not affect bacterial diversity. Interestingly, the survival and establishment of both BCAs showed opposite trends depending on the soil type, indicating that the physicochemical properties of soils have a role on BCA establishment. Fungal co-occurrence networks were less complex than bacterial networks, but highly impacted by Ta SC1 application. Soils treated with Ta SC1, presented more complex and stable co-occurrence networks, with a higher number of positive correlations. Induced grapevine defenses also differed according to the soil, being more affected by BCA inoculation on sandy soil. The findings of this research emphasize the complex relationships among microorganisms in the rhizosphere, and highlight the significance of taking into account various factors like soil type, sampling time, and BCA treatment, and their influence on the structure and dynamics of microbial communities.

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Successional Change of the Fungal Microbiome Pine Seedling Roots Inoculated With Tricholoma matsutake

Cited by 13 publications

References 98 publications

Pyrosequencing and Taxonomic Composition of the Fungal Community from Soil of Tricholoma matsutake in Gyeongju

Pyrosequencing and Taxonomic Composition of the Fungal Community from Soil of Tricholoma matsutake in Gyeongju

Biocontrol agents establishment and their impact on rhizosphere microbiome and induced grapevine defenses is highly soil-dependent

Biocontrol agents establishment and their impact on rhizosphere microbiome and induced grapevine defenses is highly soil-dependent

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