Successful vaccination against Leishmania donovani infection in Indian langur using alum-precipitated autoclaved Leishmania major with BCG

Misra, Aditi; Dube, Anuradha; Srivastava, Bindu; Sharma, Preeti; Srivastava, J. K.; Katiyar, J. C.; Naik, Sita

doi:10.1016/s0264-410x(01)00058-5

Cited by 80 publications

(56 citation statements)

References 28 publications

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“…Aluminium salts are inexpensive, safe and simple to formulate (BOMFORD, 1989). Alum has been previously used in leishmania candidate vaccines alone or in combination with other adjuvants (KENNEY et al, 1999;MISRA et al, 2001;TONUI et al, 2004). Montanide ISA 720 (MISA 720) has been recommended by the manufacturer for clinical trials in humans (GOMEZ et al, 1999 as an adjuvant is regarded as an acceptable practice in humans, and at present this adjuvant is routinely used in vaccination and immunotherapy trials against leishmaniasis (CONVIT et al, 1989;BAHAR et al, 1996;SHARIFI et al, 1998;MOMENI et al, 1999;KHALIL et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Subcutaneous immunization against Leishmania major - infection in mice: efficacy of formalin-killed promastigotes combined with adjuvants

Mutiso

Macharia

Mutisya

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYFormalin-killed promastigotes (FKP) of Leishmania major, in combination with Montanide ISA 720 (MISA), BCG or alum were used in vaccination of an inbred murine model against cutaneous leishmaniasis (CL). Significant and specific increases in anti-FKP IgG responses were detected for both alum-FKP and BCG-FKP compared to MISA-FKP (p < 0.001). Significant increases in splenic lymphocyte recall proliferation was obtained in the MISA-FKP vaccinated mice compared to alum-FKP or BCG-FKP vaccinated groups (p < 0.01). The highest interferon-γ responses were observed in the BCG-FKP group followed by the MISA-FKP while the alum-FKP gave the least responses. Significantly reduced lesion sizes were obtained in the MISA-FKP group compared to the BCG/ alum adjuvants-FKP vaccinated groups. Although the BCG-FKP group showed the highest IFN-γ responses, it failed to control cutaneous lesions. Significant reductions in parasite numbers were observed in the MISA-FKP and BCG-FKP vaccinated groups (p < 0.001). There was a good correlation between parasite burden and IFN-γ level indicating IFN-γ response as a sensitive parameter of the immune status. In conclusion, MISA-FKP is the most efficacious vaccine formulation against murine cutaneous leishmaniasis.

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Section: Introductionmentioning

confidence: 99%

Subcutaneous immunization against Leishmania major - infection in mice: efficacy of formalin-killed promastigotes combined with adjuvants

Mutiso

Macharia

Mutisya

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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“…This confirms the results observed in our earlier study on mice (MUTISO et al, 2010b) where vaccination with BCG was associated with adverse reaction. Other studies involving the use of the monkey system have also associated BCG with severe reactions (MISRA et al, 2001). Lymphadenopathy was also an undesirable feature in the use of BCG as an adjuvant in this study.…”

Section: Discussionmentioning

confidence: 68%

“…Remarkable levels of TNF-α and IFN-γ induced by groups vaccinated with antigen containing Alum-BCG and MISA indicates the superiority of these adjuvants in the selective induction of Th1 immune responses. Alum-BCG has been indicated to induce strong Th1 immune responses in Leishmania vaccine studies (MISRA et al, 2001;KAMIL et al, 2003). In malaria, HIV and cancer vaccine studies, MISA 720 has been indicated to induce high Th1 immune responses important for the control of these diseases (KENNEY & ELDELMAN, 2003).…”

Section: Discussionmentioning

confidence: 99%

Safety and skin delayed-type hypersensitivity response in vervet monkeys immunized with Leishmania donovani sonicate antigen delivered with adjuvants

Mutiso

Macharia

Taracha

et al. 2012

Rev. Inst. Med. trop. S. Paulo

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SUMMARYIn this study, we report on the safety and skin delayed-type hypersensitivity (DTH), responses of the Leishmania donovani whole cell sonicate antigen delivered in conjunction with alum-BCG (AlBCG), Montanide ISA 720 (MISA) or Monophosphoryl lipid A (MPLA) in groups of vervet monkeys. Following three intradermal injections of the inoculums on days 0, 28 and 42, safety and DTH responses were assessed. Preliminary tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) levels were also measured and these were compared with DTH. Only those animals immunized with alum-BCG reacted adversely to the inoculum by producing ulcerative erythematous skin indurations. Non-parametric analysis of variance followed by a post-test showed significantly higher DTH responses in the MISA+Ag group compared with other immunized groups (p < 0.001). The MPLA+Ag group indicated significantly lower DTH responses to the sonicate antigen compared with the AlBCG+Ag group. There was a significant correlation between the DTH and cytokine responses (p < 0.0001). Based on this study we conclude that Leishmania donovani sonicate antigen containing MISA 720 is safe and is associated with a strong DTH reaction following immunization.

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“…Furthermore, disease mimicking human VL was established in langur monkeys (Presbytis entellus) (Dube et al 1999), vervet monkeys (Cercopithecus aethiops) (Binhazim et al 1993 and macaques (Macaca mulatta) (Porrozzi et al 2006). The L. donovani-langur monkey model has also been explored to assess different vaccine formulations against VL (Dube et al 1998, Misra et al 2001.…”

Section: Vaccine Studies Against Leishmaniasismentioning

confidence: 99%

The utility of rhesus monkey (Macaca mulatta) and other non-human primate models for preclinical testing of Leishmania candidate vaccines

Grimaldi

2008

Mem. Inst. Oswaldo Cruz

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Successful vaccination against Leishmania donovani infection in Indian langur using alum-precipitated autoclaved Leishmania major with BCG

Cited by 80 publications

References 28 publications

Subcutaneous immunization against Leishmania major - infection in mice: efficacy of formalin-killed promastigotes combined with adjuvants

Subcutaneous immunization against Leishmania major - infection in mice: efficacy of formalin-killed promastigotes combined with adjuvants

Safety and skin delayed-type hypersensitivity response in vervet monkeys immunized with Leishmania donovani sonicate antigen delivered with adjuvants

The utility of rhesus monkey (Macaca mulatta) and other non-human primate models for preclinical testing of Leishmania candidate vaccines

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