Successful transplantation of a 20% partial liver graft in rats: a technical innovation

Tanaka, Hideaki; Hashizume, Kohei; Enosawa, Shin; Suzuki, Seiichi

doi:10.1016/s0022-4804(02)00090-2

Cited by 15 publications

(15 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Scarce evidence can be found on the use of alternative, modified resection protocols in rat P-OLT [67,68]. In our practice, we mostly perform the graft size reduction under the surgical microscope during back table preparation [9,37,69,70].…”

Section: Technical Issues Concerning P-olt In Ratsmentioning

confidence: 99%

See 1 more Smart Citation

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Czigány

Iwasaki²,

Yagi³

et al. 2015

Eur Surg Res

View full text Add to dashboard Cite

Background: Due to a worldwide shortage of donor organs for liver transplantation, alternative approaches, such as split and living donor liver transplantations, were introduced to increase the donor pool and reduce mortality on liver transplant waiting lists. Numerous details concerning the mechanisms and pathophysiology of liver regeneration, small-for-size syndrome, rejection, and tolerance in partial liver transplantation facilitated the development of various animal models. The high number of preclinical animal studies contributed enormously to our understanding of many clinical aspects of living donor and partial liver transplantations. Summary: Microsurgical rat models of partial orthotopic liver transplantation are well established and widely used. Nevertheless, several issues regarding this procedure are controversial, not clarified, or not yet properly standardized (graft rearterialization, size reduction techniques, etc.). The major aim of this literature review is to give the reader a current overview of rat orthotopic liver transplantation models with a special focus on partial liver transplantation. The aspects of model evolution, microsurgical training, and different technical problems are analyzed and discussed in detail. Our further aim in this paper is to elaborate a detailed publication guide in order to improve the quality of reporting in the field of rat liver transplantation according to the ARRIVE guidelines and the 3R principle. Key Messages: Partial orthotopic liver transplantation in rats is an indispensable, reliable, and cost-efficient model for transplantation research. A certain consensus on different technical issues and a significant improvement in scientific reporting are essential to improve transparency and comparability in this field as well as to foster refinement.

show abstract

Section: Technical Issues Concerning P-olt In Ratsmentioning

confidence: 99%

“…As we showed in figure 2, the spectrum of grafts applied is very wide, from the slightly reduced 70% graft [8,32] to the really challenging 20% partial liver transplant [68,71]. A liver graft is small for size when the GRWR or the graft/standard liver volume is <0.8-1.0 or 40-50%, respectively [64,72].…”

Section: Technical Issues Concerning P-olt In Ratsmentioning

confidence: 99%

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Czigány

Iwasaki²,

Yagi³

et al. 2015

Eur Surg Res

View full text Add to dashboard Cite

show abstract

“…Many growth factors and cytokines, such as IL‐6, TNF‐α, HGF, or VEGF, play important roles in liver regeneration . Several authors reported that liver regeneration was completed by 5‐7 days after partial liver graft transplantation with a peak level of the bromodeoxyuridine, proliferating cell nuclear antigen, or Ki‐67 labeling index at 1‐3days after operation in rodents . However, our experiment was the first trial to evaluate the possibility of liver regeneration in xenogeneic animals.…”

Section: Discussionmentioning

confidence: 89%

Auxiliary xenotransplantation as an in vivo bioreactor—Development of a transplantable liver graft from a tiny partial liver

Masano

Yagi

Miyachi

et al. 2019

Xenotransplantation

View full text Add to dashboard Cite

Background We established a completely novel method of auxiliary xenogeneic partial liver transplantation and examined whether liver grafts procured from Syrian hamsters regenerated in nude rats, which were used as in vivo bioreactors. Methods The hamsters and the rats were all males (n = 10). Partial liver grafts from hamsters were transplanted into nude rats in an auxiliary manner. We evaluated liver graft injury, rejection, and regeneration during 7 days after auxiliary xenogeneic partial liver transplantation. Results All rats survived until sacrifice on post‐operative day (POD) 1, 3, and 7. HE‐staining showed normal at POD1, mild periportal edema, and slight bile duct and venous endothelial inflammation at POD3, and moderate acute cellular rejection at POD7 without parenchymal necrosis. The liver regeneration rates at POD3 and 7 were 1.54 ± 0.23 and 2.54 ± 0.43, respectively. The Ki‐67 labeling index was also elevated at POD3 (27.5 ± 4.1%). Serum HGF and VEGF were elevated at POD1 and 3. ATP levels of liver grafts recovered at POD7. Conclusions These results revealed that with appropriate immunosuppressive therapy, partial liver graft regeneration occurred in a xenogeneic animal, which suggests liver grafts regenerated in xenogeneic environments, such as an in vivo bioreactor, have potential to be transplantable liver grafts for humans.

show abstract

“…The OLT method was a modified version of the technique described by Kamada and Calne 28. After systemic heparinization, the donor livers (20% partial liver) were harvested with the modified technique described by Tanaka et al,29 were flushed through the portal vein, and were stored in HTK solution alone or in HTK solution containing APC. After the removal of the livers from the recipients, the partial liver grafts were anastomosed to the suprahepatic vena cava with 8‐0 continuous sutures.…”

Section: Methodsmentioning

confidence: 99%

The cytoprotective effects of addition of activated protein C into preservation solution on small-for-size grafts in rats

et al. 2009

View full text Add to dashboard Cite

Small-for-size liver grafts are a serious obstacle for partial orthotopic liver transplantation. Activated protein C (APC), a potent anticoagulant serine protease, is known to have cell-protective properties due to its anti-inflammatory and antiapoptotic activities. This study was designed to examine the cytoprotective effects of a preservation solution containing APC on small-for-size liver grafts, with special attention paid to ischemia-reperfusion injury and shear stress in rats. APC exerted cytoprotective effects, as evidenced by (1) increased 7-day graft survival; (2) decreased initial portal pressure and improved hepatic microcirculation; (3) decreased levels of aminotransferase and improved histological features of hepatic ischemiareperfusion injury; (4) suppressed infiltration of neutrophils and monocytes/macrophages; (5) reduced hepatic expression of tumor necrosis factor ␣ and interleukin 6; (6) decreased serum levels of hyaluronic acid, which indicated attenuation of sinusoidal endothelial cell injury; (7) increased hepatic levels of nitric oxide via up-regulated hepatic endothelial nitric oxide synthesis expression together with down-regulated hepatic inducible nitric oxide synthase expression; (8) decreased hepatic levels of endothelin 1; and (9) reduced hepatocellular apoptosis by down-regulated caspase-8 and caspase-3 activities. These results suggest that a preservation solution containing APC is a potential novel and safe product for small-for-size liver transplantation, alleviating graft injury via anti-inflammatory and antiapoptotic effects and vasorelaxing conditions. Liver Transpl 16: 1-11, 2010.

show abstract

Successful transplantation of a 20% partial liver graft in rats: a technical innovation

Cited by 15 publications

References 11 publications

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Auxiliary xenotransplantation as an in vivo bioreactor—Development of a transplantable liver graft from a tiny partial liver

The cytoprotective effects of addition of activated protein C into preservation solution on small-for-size grafts in rats

Contact Info

Product

Resources

About