Successful sublingual immunotherapy with birch pollen has limited effects on concomitant food allergy to apple and the immune response to the Bet v 1 homolog Mal d 1

Kinaciyan, Tamar; Jahn‐Schmid, Beatrice; Radakovics, Astrid; Zwölfer, Bettina; Schreiber, Claudia; Francis, James N.; Ebner, Christof; Bohle, Barbara

doi:10.1016/j.jaci.2006.11.010

Cited by 127 publications

(110 citation statements)

References 29 publications

(37 reference statements)

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“…In the current study, we have cloned, sequenced, and functionally characterized the TCR a-and b-chains of a CD4 + TCC (SD334) [25][26][27][28][29][30][31][32][33][34] , confirming the general validity of our findings.…”

Section: Discussionsupporting

confidence: 74%

“…Individuals with accompanying atopic dermatitis may additionally suffer cutaneous exacerbations (e.g., aggravation of atopic eczema) upon ingestion of Bet v 1-related food allergens (13). Significantly, Bet v 1-related food allergy cannot be palliated by conventional specific immunotherapy, leaving this aspect of birch pollen allergy currently intractable (29). To contribute to a better understanding of the Bet v 1-specific T cell response, in this study we devised a novel tool, a molecularly defined and transferable Bet v 1 142-153 -specific food and pollen cross-reactive TCR, which allows the creation of biological model systems in which the Bet v 1-specific T cell response can be studied in greater detail and which can be subjected to hypothesisbased modulation.…”

Section: Discussionmentioning

confidence: 99%

“…Intracellular cytokine production was analyzed after pretreatment for 6 h with GolgiStop (1:1500; Becton Dickinson, Palo Alto, CA) using Fix and Perm (An der Grub) and using Abs specific for IFN-g (25723-PerCP; R&D Systems, Minneapolis, MN), IL-2 (MQ1-17H12-allophycocyanin; eBioscience, San Diego, CA), IL-4 (MP4-25D2-PE; Invitrogen, Camarillo, CA), IL-5 (JES1-39D10-PE; BioLegend, San Diego, CA), IL-10 (JES3-9D7; eBioscience), and IL-13 (JES10-5A2-allophycocyanin; BioLegend). [25][26][27][28][29][30][31][32][33][34] and used 72 h after transfection. For polarization experiments, aAPCs were variably transfected with single-chain IL-12::GPI.…”

Section: Recombinant Allergensmentioning

confidence: 99%

“…Monocyte-derived dendritic cells (MDDCs) were differentiated as described previously (27) [25][26][27][28][29][30][31][32][33][34][35][36] , or medium alone (control) for 3 h were generated. Subsequently, Bet v 1 142-153 -specific or Art v 1 [25][26][27][28][29][30][31][32][33][34][35][36] -specific TCRtg Jurkat cells (1 3 10 5 ) were added to 5 3 10 4 of the above-described aAPCs and cocultured for 6 h. PMA (10 27 M) plus PHA (5 mg/ml) served as positive and medium alone as negative control.…”

Section: Generation Of Monocyte-derived Dendritic Cellsmentioning

confidence: 99%

“…Subsequently, Bet v 1 142-153 -specific or Art v 1 [25][26][27][28][29][30][31][32][33][34][35][36] -specific TCRtg Jurkat cells (1 3 10 5 ) were added to 5 3 10 4 of the above-described aAPCs and cocultured for 6 h. PMA (10 27 M) plus PHA (5 mg/ml) served as positive and medium alone as negative control. Luciferase activity was assayed as described (21).…”

Section: Generation Of Monocyte-derived Dendritic Cellsmentioning

confidence: 99%

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Human TCR Transgenic Bet v 1-Specific Th1 Cells Suppress the Effector Function of Bet v 1-Specific Th2 Cells

Neunkirchner

Reichl

Schmetterer

et al. 2011

The Journal of Immunology

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Pollinosis to birch pollen is a common type I allergy in the Northern Hemisphere. Moreover, birch pollen-allergic individuals sensitized to the major birch pollen allergen Bet v 1 frequently develop allergic reactions to stone fruits, hazelnuts, and certain vegetables due to immunological cross-reactivity. The major T cell epitope Bet v 1142–153 plays an important role in cross-reactivity between the respiratory allergen Bet v 1 and its homologous food allergens. In this study, we cloned and functionally analyzed a human αβ TCR specific for the immunodominant epitope Bet v 1142–153. cDNAs encoding TCR α- and β-chains were amplified from a Bet v 1142–153-specific T cell clone, introduced into Jurkat T cells and peripheral blood T lymphocytes of allergic and nonallergic individuals, and evaluated functionally. The resulting TCR transgenic (TCRtg) T cells responded in an allergen-specific and costimulation-dependent manner to APCs either pulsed with Bet v 1142–153 peptide or coexpressing invariant chain::Bet v 1142–153 fusion proteins. TCRtg T cells responded to Bet v 1-related food and tree pollen allergens that were processed and presented by monocyte-derived dendritic cells. Bet v 1142–153-presenting but not Bet v 14–15-presenting artificial APCs coexpressing membrane-bound IL-12 polarized allergen-specific TCRtg T cells toward a Th1 phenotype, producing high levels of IFN-γ. Coculture of such Th1-polarized T cells with allergen-specific Th2-differentiated T cells significantly suppressed Th2 effector cytokine production. These data suggest that human allergen-specific TCR can transfer the fine specificity of the original T cell clone to heterologous T cells, which in turn can be instructed to modulate the effector function of the disease initiating/perpetuating allergen-specific Th2-differentiated T cells.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Recombinant Allergensmentioning

confidence: 99%

Section: Generation Of Monocyte-derived Dendritic Cellsmentioning

confidence: 99%

Section: Generation Of Monocyte-derived Dendritic Cellsmentioning

confidence: 99%

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Human TCR Transgenic Bet v 1-Specific Th1 Cells Suppress the Effector Function of Bet v 1-Specific Th2 Cells

Neunkirchner

Reichl

Schmetterer

et al. 2011

The Journal of Immunology

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Sublingual immunotherapy for treating allergic conjunctivitis

Calderón

Penagos

Sheikh

et al. 2009

Cochrane Database of Systematic Reviews

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Immunotherapy (oral and sublingual) for food allergy to fruits

Yepes-Núñez

Zhang

Figuls

et al. 2015

Cochrane Database of Systematic Reviews

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BackgroundFood allergy is an abnormal immunological response following exposure (usually ingestion) to a food. Elimination of the allergen is the principle treatment for food allergy, including allergy to fruit. Accidental ingestion of allergenic foods can result in severe anaphylactic reactions. Allergen-specific immunotherapy (SIT) is a specific treatment, when the avoidance of allergenic foods is problematic. Recently, studies have been conducted on di erent types of immunotherapy for the treatment of food allergy, including oral (OIT) and sublingual immunotherapy (SLIT). ObjectivesTo determine the e icacy and safety of oral and sublingual immunotherapy in children and adults with food allergy to fruits, when compared with placebo or an elimination strategy. Search methodsThe Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE, EMBASE, CINAHL, and AMED were searched for published results along with trial registries and the Journal of Negative Results in BioMedicine for grey literature. The date of the most recent search was July 2015. Selection criteriaRandomised controlled trials (RCTs) comparing OIT or SLIT with placebo or an elimination diet were included. Participants were children or adults diagnosed with food allergy who presented immediate fruit reactions.Immunotherapy (oral and sublingual) for food allergy to fruits (Review)

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Successful sublingual immunotherapy with birch pollen has limited effects on concomitant food allergy to apple and the immune response to the Bet v 1 homolog Mal d 1

Cited by 127 publications

References 29 publications

Human TCR Transgenic Bet v 1-Specific Th1 Cells Suppress the Effector Function of Bet v 1-Specific Th2 Cells

Human TCR Transgenic Bet v 1-Specific Th1 Cells Suppress the Effector Function of Bet v 1-Specific Th2 Cells

Sublingual immunotherapy for treating allergic conjunctivitis

Immunotherapy (oral and sublingual) for food allergy to fruits

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