Successful Small Diameter Arterial Grafting Using Cryopreserved Allograft Arteries

Eskew, Thomas D.; Ollerenshaw, Jeremy D.; Dawson, Patti E.; Black, Kirby S.; Abbott, W M; Lust, Robert M.

doi:10.1097/00002480-199703000-00247

Cited by 7 publications

(2 citation statements)

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“…In animal models, various staining methods have been used for morphometric and descriptive analysis, depending on the preferences of each author, such as the combination of the elastic fibres stain with a connective tissue stain such as Masson's trichrome elastin stain (Gallo et al., 1999; Roqué et al., 2000; Worthley et al., 2000) and Van Gieson stain (Yasuda et al., 2000). However, there are authors who have preferred to use a stain only for elastic fibres, such as Verhoeff stain (Nugent et al., 1999; Garasic et al., 2000) or orcein stain (Eskew et al., 1997; Feldman et al., 2000). In this study, the choice of orcein stain was complemented with haematoxylin eosin and Masson's trichrome to describe the remaining vascular components.…”

Section: Discussionmentioning

confidence: 99%

“…In our study, as in most others, the morphometric analysis was based on calculating the area of the layers of the arterial wall. Some studies also include measuring the thickness of the layers (Ruengsakulrach et al., 1999) or the diameter of the lumen (Eskew et al., 1997). All the same, vascular structure is not completely regular and, therefore, measurement of the area is more precise.…”

Section: Discussionmentioning

confidence: 99%

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Histological Basis of the Porcine Femoral Artery for Vascular Research

Solanes

Rigol

Ramírez³

et al. 2005

Anat Histol Embryol

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The similarities between the porcine and human cardiovascular systems make the pig a useful animal model for the study of vascular biology. However, a standardized method is needed to describe the normal histological properties of porcine arteries in order to evaluate pathologic lesions in future studies. Descriptive and morphometric analyses were done on 16 porcine femoral arteries. For these purposes, three histological stains (haematoxylin eosin, Masson's trichrome, and orcein), four immunohistochemical methods (using antibodies anti-alpha-actin, anti-CD3, anti-L1 and anti-lysozyme), and a glycohistochemical method (using Dolichos biflorus lectin) were performed. The porcine femoral arteries evaluated had a mean total area of 6.25 +/- 1.99 mm(2) and a diameter of 2.79 +/- 0.41 mm. The majority of the total area was occupied by the medial layer (42.97 +/- 5.38%) and was mainly constituted by smooth muscle cells (94.58 +/- 2.65%). All the cell markers used reacted with porcine paraffin-embedded tissue. However, the anti-lysozyme antibody was excluded from this histological analysis because of cytoplasmatic reactivity in smooth muscle cells. In summary, this study proposes histological methods to describe the normal characteristics of the porcine femoral artery and raises the possibility of applying this methodology in future studies on porcine vascular research.

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