Cryopreservation alters antigenicity of allografts in a porcine model of transplant vasculopathy

Solanes, Núria; Rigol, Montserrat; Castellà, Manuel; Khabiri, Ebrahim; Ramírez, José; Segalés, Joaquím; Roqué, Mercè; Agustí, Elba; Pérez‐Villa, Félix; Roig, Eulàlia; Pomar, José L.; Sanz, Ginés; Heras, Magda

doi:10.1016/j.transproceed.2004.10.053

Cited by 21 publications

(17 citation statements)

References 22 publications

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“…3; Martin et al 1994;Miller et al 1993). Thrombogenicity and antigenicity after thawing nevertheless remain unclear (Muto et al 2010;Solanes et al 2004). Thrombus formation was not seen in our grafts.…”

Section: Discussionmentioning

confidence: 72%

Neointimal hyperplasia in allogeneic and autologous venous grafts is not different in nature

Albert

Hartmann

Wagner

et al. 2015

Histochem Cell Biol

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Neointimal hyperplasia, transplant rejection and thus immunogenicity of allografts are possible reasons for poorer patency rates in cryopreserved venous allografts for peripheral bypass surgery in comparison with autologous venous grafts. To expand the limited knowledge from human allografts, we histologically investigated allogeneic and autologous venous grafts in arterial location. Specimens of allogeneic and autologous venous graft stenosis, harvested 6 months after bypass implantation, were immunohistochemically characterized. Examination of the lesions showed a uniform morphological pattern. A continuous endothelial layer, tissue fibrosis and a thickened neointima with monocytes and dedifferentiated vascular smooth muscle cells were seen in both conduits with very low cell turnover and the absence of acute and chronic inflammation. Neoangiogenesis with CD34-positive endothelium was abundant in the vessel media. The morphological patterns of allogeneic and autologous neointima formation are similar. Consequently, neointimal hyperplasia in venous grafts may reflect a uniform physiological host response of non-immunological factors with the reasons for poorer clinical outcome of cryopreserved allografts yet to be elucidated.

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Section: Discussionmentioning

confidence: 72%

Neointimal hyperplasia in allogeneic and autologous venous grafts is not different in nature

Albert

Hartmann

Wagner

et al. 2015

Histochem Cell Biol

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“…It is yet unclear whether cryopreserved arterial allografts are less immunogenic than fresh tissue (Nagasaka et al 2005;Oei et al 2002;Solanes et al 2004); in fact cryopreservation damages structurally and functionally endothelial and smooth muscle cells Song et al 1995;Hunt et al 1994;Pegg et al 1997;Faggioli and Ricotta 1994;Pasquinelli et al 2006;Mü llerSchweinitzer et al 1997;Và zquez et al 2004a;Và zquez et al 2004b;Stanke et al 1998;Langerak et al 2001). Conseguently, this procedure reduces the number of grafted cells which constitutively express HLA class I antigens (crucial for tissue histocompatibility); moreover, damage of surface endothelium, caused by cryopreservation (Faggioli and Ricotta 1994) might lead to the loss of a potential alternative source of antigen presentating cells.…”

Section: Discussionmentioning

confidence: 99%

The “in situ” expression of Human Leukocyte Antigen Class I antigens is not altered by cryopreservation in human arterial allografts

Pasquinelli

Pistillo²,

Ricci

et al. 2006

Cell Tissue Banking

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This study was aimed to establish whether the cryopreservation procedure we currently use in clinics can modify arterial homograft antigenicity. To this purpose, we performed an immunohistochemical study on fresh and cryopreserved human arterial homografts to visualize the expression of HLA class I heavy and light chains "in situ" by using the HC-10 and Namb-1 monoclonal antibodies. Human femoral arteries and thoracic aortas were harvested from 18 heart-beating donors and sampled before and after cryopreservation. Arterial segments were frozen in liquid nitrogen vapors in a controlled rate freezing system. After thawing, samples were processed for routine immunohistochemistry. To standardize immunostaining, flow-cytometry indirect immunofluorescence analysis was performed on HUVEC; immunohistochemistry of human ovarian cortical vessels was performed as an additional positive control. Negative controls were performed by omitting tissue incubation with primary antibodies. HLA-class I antigens were markedly expressed by endothelial cells lining surface intima and adventitial vasa vasorum; a moderate expression was found in medial smooth muscle cells. Except for the surface unreactivity caused by loss of endothelium, results from cryopreserved arterial allografts were strictly comparable to those observed in fresh, unfrozen tissues. These results support the view that cryopreserved arterial allografts are immunogenic as their fresh counterparts; apart from smooth muscle cells which retained a moderate expression of HLA class I antigens following cryopreservation, our study suggests that the highly HC-10 positive endothelial cells we found to line the rich adventitial network of vasa vasorum are expected to be one of the major targets of the serological response in the recipient.

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“…Immunosuppressant-free allotransplantation was then undertaken to evaluate the impact of cryopreservation on tracheal antigenicity in dogs. Methods: Tracheal rings [7][8] were removed from mongrel adult dogs for cryopreservation (1-10 months, −85 ℃) and grafting. Before transplantation, one ring was sectioned from each end for histologic examination.…”

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confidence: 99%

Effects of cryopreservation on tracheal allograft antigenicity in dogs

Huang

et al. 2017

J. Thorac. Dis.

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Background: Prolonged cryopreservation (~8-10 months) has been shown to reduce the antigenicity of tracheal allografts due to subsequent denuding of epithelium. In the present study, tracheal epithelium was assessed after variable periods of cryopreservation. Immunosuppressant-free allotransplantation was then undertaken to evaluate the impact of cryopreservation on tracheal antigenicity in dogs. Methods: Tracheal rings [7][8] were removed from mongrel adult dogs for cryopreservation (1-10 months, −85 ℃) and grafting. Before transplantation, one ring was sectioned from each end for histologic examination. The residual five-ring segments of mediastinal trachea were then transplanted into recipient dogs after 1-7 months (group 1, n=9) or 8-10 months (group 2, n=6) of cryopreservation.Anastomotic sites and allografts were covered by omental pedicles. No immunosuppressants whatsoever were administered.Results: In microscopic views, the ciliated tracheal epithelium of most grafts showed variable loss but was generally intact after cryopreservation, still demonstrating major histocompatibility complex (MHC)-II positivity. By postoperative bronchoscopy, allografts in both groups had largely developed lethal strictures. In group 1, eight dogs were sacrificed or died within 50 days post-transplantation, whereas survival times in group 2 were somewhat longer, with three dogs surviving for >60 days. Upon sacrifice, histologic preparations of grafted tissue in both groups were typically denuded of epithelium, with marked lymphocytic/monocytic submucosal infiltrates. Tracheal cartilage had been absorbed or destroyed.Conclusions: After cryopreservation, some degree of tracheal epithelium loss maybe expected, but complete denudation is not obligatory. Retained epithelial antigenicity is thus capable of triggering rejection, resulting in transplant failures. Although prolonging transplant survival to an extent, fatal rejection of tracheal allografts was not preventable by prior cryopreservation.

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Cryopreservation alters antigenicity of allografts in a porcine model of transplant vasculopathy

Cited by 21 publications

References 22 publications

Neointimal hyperplasia in allogeneic and autologous venous grafts is not different in nature

Neointimal hyperplasia in allogeneic and autologous venous grafts is not different in nature

The “in situ” expression of Human Leukocyte Antigen Class I antigens is not altered by cryopreservation in human arterial allografts

Effects of cryopreservation on tracheal allograft antigenicity in dogs

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