Successful pregnancy following transfer of feline embryos derived from vitrified immature cat oocytes using ‘stepwise’ cryoprotectant exposure technique

Tharasanit, Theerawat; Manee-in, Sukanya; Buarpung, Sirirak; Chatdarong, Kaywalee; Lohachit, Chainarong; Techakumphu, Mongkol

doi:10.1016/j.theriogenology.2011.06.014

Cited by 36 publications

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“…Despite the fact that Tharasanit et al (2011) andPope et al (2012) reported a birth of live kittens obtained from vitrified cat oocytes, cryopreservation of immature or mature cat oocytes was not proven to be a reliable and repeatable tool so far. In the few published works, the cleavage rate of vitrified-warmed domestic cat oocytes ranged between 14% and 25% (Merlo et al 2008;Comizzoli et al 2009;Cocchia et al 2010;Tharasanit et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…In the few published works, the cleavage rate of vitrified-warmed domestic cat oocytes ranged between 14% and 25% (Merlo et al 2008;Comizzoli et al 2009;Cocchia et al 2010;Tharasanit et al 2011). Cat oocyte cryopreservation is still considered to be an experimental technique (Luvoni 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Cryopreservation of domestic cat oocytes is also an important part of ART. Successful cryopreservation of female gametes would allowed to overcome time and space differences which are often an obstacle for research and field work of feline species.The domestic cat (Felis catus) is an easily obtainable biomedical model for studies on wild cat species and it has been demonstrated that many domestic cat ART protocols can be applied in wild felids (Pope 2000).Despite the fact that Tharasanit et al (2011) andPope et al (2012) reported a birth of live kittens obtained from vitrified cat oocytes, cryopreservation of immature or mature cat oocytes was not proven to be a reliable and repeatable tool so far. In the few published works, the cleavage rate of vitrified-warmed domestic cat oocytes ranged between 14% and 25% (Merlo et al 2008;Comizzoli et al 2009;Cocchia et al 2010;Tharasanit et al 2011).…”

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confidence: 99%

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Vitrification of Domestic Cat Oocytes – Effect on Viability and Integrity of Subcellular Structures

Mikołajewska

Müller

Niżański

et al. 2012

Reprod Domestic Animals

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ContentsCryopreservation of female gametes is a crucial part of assisted reproduction techniques. The domestic cat is a model for both wild felids and human research. The aim of this study was to evaluate the effect of different vitrification protocols applied to feline oocytes at different maturational stages on the preservation of oocyte viability and integrity of subcellular structures. The vitrification solutions consisted of 20% ethylene glycol, 20% DMSO, 20% FCS, and 1.5 M Trehalose with and without 10% Ficoll PM-70. Markers for cell viability (PI/ FDA), cytoskeleton organization (Anti-a-Tubulin-FITC antibody, Phalloidin-TRITC), as well as nuclear configuration (DAPI) were used for evaluation of vitrified-warmed oocytes. Our results show that 52% and 41% of live mature and immature oocytes, respectively and until 32% of microtubules, 28% of nuclear configuration and 36% of microfilaments in the normal pattern can be obtained with protocol described in this paper. According to our data, Ficoll PM-70 essentially improves the oocytes survival upon vitrification. IntroductionAssisted reproduction techniques (ART) as in vitro fertilization and embryo transfer are important for wild animal conservation programs. Cryopreservation of domestic cat oocytes is also an important part of ART. Successful cryopreservation of female gametes would allowed to overcome time and space differences which are often an obstacle for research and field work of feline species.The domestic cat (Felis catus) is an easily obtainable biomedical model for studies on wild cat species and it has been demonstrated that many domestic cat ART protocols can be applied in wild felids (Pope 2000).Despite the fact that Tharasanit et al. (2011) andPope et al. (2012) reported a birth of live kittens obtained from vitrified cat oocytes, cryopreservation of immature or mature cat oocytes was not proven to be a reliable and repeatable tool so far. In the few published works, the cleavage rate of vitrified-warmed domestic cat oocytes ranged between 14% and 25% (Merlo et al. 2008;Comizzoli et al. 2009;Cocchia et al. 2010;Tharasanit et al. 2011). Cat oocyte cryopreservation is still considered to be an experimental technique (Luvoni 2006).The aim of this study was to evaluate the effect of different vitrification protocols applied to feline oocytes at different maturational stages on the preservation of oocyte viability and integrity of subcellular structures. The assessment by fluorescent markers of oocyte viability, cytoskeletal organization and nuclear configuration in vitrified and fresh oocytes was aimed to identify the damaging effects of cryopreservation.To achieve a very high cooling rate, which is necessary to prevent ice formation, the volume of the vitrification solution should be minimized by using devices specially designed for vitrification. In our study we focused on two of the most commonly used techniques -cryoloop and cryotop. Materials and MethodsAll chemicals were purchased from Sigma-Aldrich Chemie Gmbh (Munich, Germany), unless othe...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Vitrification of Domestic Cat Oocytes – Effect on Viability and Integrity of Subcellular Structures

Mikołajewska

Müller

Niżański

et al. 2012

Reprod Domestic Animals

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“…Likewise deserving of attention are alternative oocyte cryopreservation methods, including ultrarapid or vitrification protocols (on electron microscope grids and cryoloops; Saragusty and Arav 2011). Among the most non-traditional mammal models, successful vitrification of immature oocytes followed by IVF, ET and pregnancy has been reported recently in the domestic cat (Tharasanit et al 2011). …”

Section: Examples In Comparative Oocyte Traits and Cryosensitivitymentioning

confidence: 99%

Mammalian fertility preservation through cryobiology: value of classical comparative studies and the need for new preservation options

Comizzoli

Wildt

2014

Reprod. Fertil. Dev.

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Human-related fertility preservation strategies have enormous potential for helping sustain and protect other species, especially to assist managing or ‘rescuing’ the genomes of genetically valuable individuals, including endangered species. However, wider-scale applications are limited by significant physiological variations among species, as well as a lack of fundamental knowledge of basic reproductive traits and cryosensitivity. Systematic and comparative cryopreservation studies (e.g. on membrane biophysical properties and resilience to freezing temperatures) are required to successfully recover gametes and gonadal tissues after thawing and eventually produce healthy offspring. Such data are currently available for humans and a few laboratory and livestock animals, with virtually all other species, including wildlife, having gone unstudied. Interestingly, there also are commonalities among taxa that allow a protocol developed for one species to provide useful information or guidance for another. However, when a rare animal unexpectedly dies there is no time for a prospective understanding of that species’ biophysical traits. Because the odds of success will be much lower in such instances, it is essential that more fundamental studies be directed at more species. But also worthwhile is thinking beyond these systematic characterisations to consider the potential of a ‘universal preservation protocol’ for animal biomaterials.

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“…Recently, the first pregnancy was established in a recipient receiving embryos derived from vitrified immature oocytes (Tharasanit et al. ), but foetal resorption occurred during mid‐gestation. The commercial introduction of innovative cryodevices, such as the Cryotop, prompted further investigations and, very recently, the first kittens, were born after vitrification of mature oocytes, ICSI and transfer of derived embryos into recipients (Pope et al.…”

Section: Introductionmentioning

confidence: 99%

Cryosurvival of Ex Situ and In Situ Feline Oocytes

Luvoni

2012

Reprod Domestic Animals

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Contents Cryosurvival of feline oocytes preserved as isolated cells (ex situ) or enclosed in ovarian follicles (in situ) has been demonstrated, and significant advances have recently been achieved. However, an ideal protocol for oocyte cryopreservation has not been established to date because of extreme sensitivity of the structural complex to chilling injury. Several factors, such as stage of maturation, membrane permeability and plasticity of the cytoskeleton, affect cryosurvival of the oocyte. Also, intercellular communications between cumulus cells and oocyte are compromised after freezing or vitrification of ex situ or in situ cumulus–oocyte complexes, which has a detrimental effect on oocyte maturational competence. Despite these issues, embryo development, pregnancies and live kittens have been obtained after in vitro fertilization (by ICSI) and transfer of embryos derived from cryopreserved oocytes. It is a general belief that the efficiency of cryopreservation would increase through a better understanding of oocyte responses to cryoprotectants, cooling rates and all the physical events occurring during the exposure of feline oocytes to low temperatures. Cryobanking of feline oocytes would significantly contribute to the preservation of rare genotypes and to the maintenance of a valuable source of genetic material for research applications.

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Successful pregnancy following transfer of feline embryos derived from vitrified immature cat oocytes using ‘stepwise’ cryoprotectant exposure technique

Cited by 36 publications

References 48 publications

Vitrification of Domestic Cat Oocytes – Effect on Viability and Integrity of Subcellular Structures

Vitrification of Domestic Cat Oocytes – Effect on Viability and Integrity of Subcellular Structures

Mammalian fertility preservation through cryobiology: value of classical comparative studies and the need for new preservation options

Cryosurvival of Ex Situ and In Situ Feline Oocytes

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