Successful horizontal transfer of Wolbachia symbionts between Trichogramma wasps

Grenier, S.; Pintureau, Bernard; Heddi, Abdelaziz; Lassablière, François; Jager, C.R.; Louis, C.; Khatchadourian, Chaqué

doi:10.1098/rspb.1998.0455

Cited by 87 publications

(72 citation statements)

References 22 publications

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“…The specific primers designed in this study will be very useful for distinguishing a possible similar Wolbachia found in T. atopovirilia. The utility of primer specificity in horizontal transmission studies where different hosts are used has been mentioned by Grenier et al (1998).…”

Section: Discussionmentioning

confidence: 99%

Phylogeny of the Trichogramma endosymbiont Wolbachia, an alpha-proteobacteria (Rickettsiae)

Almeida

Stouthamer²

2017

Braz. J. Biol.

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Wolbachia (Hertig) endosymbionts are extensively studied in a wide range of organisms and are known to be transmitted through the egg cytoplasm to the offsping. Wolbachia may cause several types of reproductive modifications in arthropods. In Trichogramma species, parthenogenesis-inducing Wolbachia bacteria allow females wasps to produce daughters from unfertilized eggs and these bacteria are present in at least 9% of all Trichogramma species. Phylogenetic studies have led to the subdivision of the Wolbachia clade in five supergroups (A, B, C, D and E) and Wolbachia from Trichogramma belong to supergroup B. Here, using the wsp gene, four groups of Wolbachia that infect Trichogramma species were distinguished and the addition of a new group "Ato" was suggested due to the addition of Wolbachia from Trichogramma atopovirilia (Oatman and Platner). Specific primers were designed and tested for the "Ato" group. Seventy-five percent of all evaluated Wolbachia strains from Trichogramma fell within "Sib" group.Keywords: symbiont, egg parasitoid, DNA sequencing, phylogenetic relationships. and Platner, 1983). Primers específicos foram construídos e testados para o grupo "Ato". Setenta e cinco por cento de todas as linhagens de Wolbachia que infectam Trichogramma se enquadraram dentro do grupo "Sib". Filogenia do endosimbiontePalavras-chave: simbionte, parasitoide de ovos, sequenciamento de DNA, filogenia.

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Section: Discussionmentioning

confidence: 99%

Phylogeny of the Trichogramma endosymbiont Wolbachia, an alpha-proteobacteria (Rickettsiae)

Almeida

Stouthamer²

2017

Braz. J. Biol.

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“…However, microinjection in embryos might not be representative of the (as yet unknown) routes followed by horizontal transmission in the wild. For example it has been demonstrated that Wol bachia can also be experimentally transmitted by injec tion in nymphs of Trichogramma (Trichogrammatidae) wasps (Grenier et al, 1998) or in the adult in terrestrial isopods (Juchault et al, 1994). in the case of the wood louse Armadillidium vulgare (Latr.…”

Section: Success Rate Of the Trans-infectionmentioning

confidence: 99%

Wolbachia injection from usual to naive host in Drosophila simulans (Diptera: Drosophilidae)

Poinsot¹,

Merçot²

2001

Eur. J. Entomol.

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Abstract. Wolbachiapipientis (Hertig) (Rickettsiaceae) is an endocellular bacterium infecting numerous species of arthropods. The bacterium is harboured by males and females but is only transmitted maternally because spermatocytes shed their Wolbachia during maturation. The presence of this endosymbiont can lead to feminisation of the host, parthenogenesis, male-killing or reproductive incompatibility called cytoplasmic incompatibility (Cl). Although Wolbachia transmission is exclusively maternal, phylogenetic evi dence indicates that very rare inter-species transmission events have taken place. Horizontal transmission is possible in the labora tory by transferring cytoplasm from infected to uninfected eggs. Using this technique, we have artificially infected lines of the fruit fly Drosophila simulans Sturtevant (Drosophilidae). Recipient lines came from two different D. simulans populations. One ("naive" host) is not infected in the wild. The other ("usual" host) is a population naturally carrying Wolbachia in the wild. In this second case, recipient flies used in the experiment came from a stock culture that had been cured off its infection beforehand by an antibi otic treatment. Infected D. simulans laboratory stocks were used as donors. We assessed the three following parameters: (i) trans infection success rate (ratio of infected over total female zygote having survived the injection), (ii) level of cytoplasmic incompatibility expressed by trans-infected males three generations post-trans-infection, and (iii) infection loss rate over time in trans-infected lines (percentage of lines having lost the infection after 20 to 40 generations). We observed that parameter (i) did not differ significantly whether the recipient line came from a "naive" or a "usual" host population. However, both (ii) and (iii) were sig nificantly higher in the "naive" trans-infected stock, which is in agreement with earlier theoretical considerations.

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“…For instance, the 'popcorn' strain of Wolbachia causes CI and reduces adult lifespan both in its native host, Drosophila melanogaster, and in a very distantly related host, Aedes aegypti, to which it has been experimentally transferred (McMeniman et al, 2009). In other cases, however, Wolbachia fail to express the original phenotype and sometimes express entirely novel phenotypes (for example, Grenier et al, 1998;van Meer and Stouthamer, 1999;Sasaki et al, 2002;Sasaki et al, 2005;Jaenike, 2007). From an applied standpoint, predictability of Wolbachia phenotypic effects is highly desirable in programs using these endosymbionts for control or genetic manipulation of insect populations (Cook et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Loss of reproductive parasitism following transfer of male-killing Wolbachia to Drosophila melanogaster and Drosophila simulans

et al. 2012

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Wolbachia manipulate insect host biology through a variety of means that result in increased production of infected females, enhancing its own transmission. A Wolbachia strain (wInn) naturally infecting Drosophila innubila induces male killing, while native strains of D. melanogaster and D. simulans usually induce cytoplasmic incompatibility (CI). In this study, we transferred wInn to D. melanogaster and D. simulans by embryonic microinjection, expecting conservation of the male-killing phenotype to the novel hosts, which are more suitable for genetic analysis. In contrast to our expectations, there was no effect on offspring sex ratio. Furthermore, no CI was observed in the transinfected flies. Overall, transinfected D. melanogaster lines displayed lower transmission rate and lower densities of Wolbachia than transinfected D. simulans lines, in which established infections were transmitted with near-perfect fidelity. In D. simulans, strain wInn had no effect on fecundity and egg-to-adult development. Surprisingly, one of the two transinfected lines tested showed increased longevity. We discuss our results in the context of host-symbiont co-evolution and the potential of symbionts to invade novel host species.

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Successful horizontal transfer of Wolbachia symbionts between Trichogramma wasps

Cited by 87 publications

References 22 publications

Phylogeny of the Trichogramma endosymbiont Wolbachia, an alpha-proteobacteria (Rickettsiae)

Phylogeny of the Trichogramma endosymbiont Wolbachia, an alpha-proteobacteria (Rickettsiae)

Wolbachia injection from usual to naive host in Drosophila simulans (Diptera: Drosophilidae)

Loss of reproductive parasitism following transfer of male-killing Wolbachia to Drosophila melanogaster and Drosophila simulans

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