Successful co-infection of two different baculovirus species in the same cell line reveals a potential strategy for large in vitro production

Sanches, Márcio Martinello; Guimarães, Gregório Corrêa; Sihler, W.; Souza, Marlinda Lobo de

doi:10.1007/s42770-021-00622-z

Cited by 4 publications

(7 citation statements)

References 31 publications

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“…In these cases, applications of cooccluded mixtures of HearSNPV and HearMNPV might allow the simultaneous control of both pests, although this required verification in careful field testing. Moreover, other researchers are exploring the application of virus coocclusion technology for the control of complexes of lepidopteran pests in certain crops [58,59]. Commercial producers have also addressed this issue by mixing OBs of different viruses to create products capable of controlling complexes of pests [60].…”

Section: Discussionmentioning

confidence: 99%

Coocclusion of Helicoverpa armigera Single Nucleopolyhedrovirus (HearSNPV) and Helicoverpa armigera Multiple Nucleopolyhedrovirus (HearMNPV): Pathogenicity and Stability in Homologous and Heterologous Hosts

Arrizubieta

Simón

Ricarte-Bermejo

et al. 2022

Viruses

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Helicoverpa armigera single nucleopolyhedrovirus (HearSNPV) is a virulent pathogen of lepidopterans in the genera Heliothis and Helicoverpa, whereas Helicoverpa armigera multiple nucleopolyhedrovirus (HearSNPV) is a different virus species with a broader host range. This study aimed to examine the consequences of coocclusion of HearSNPV and HearMNPV on the pathogenicity, stability and host range of mixed-virus occlusion bodies (OBs). HearSNPV OBs were approximately 6-fold more pathogenic than HearMNPV OBs, showed faster killing by approximately 13 h, and were approximately 45% more productive in terms of OB production per larva. For coocclusion, H. armigera larvae were first inoculated with HearMNPV OBs and subsequently inoculated with HearSNPV OBs at intervals of 0–72 h after the initial inoculation. When the interval between inoculations was 12–24 h, OBs collected from virus-killed insects were found to comprise 41–57% of HearSNPV genomes, but the prevalence of HearSNPV genomes was greatly reduced (3–4%) at later time points. Quantitative PCR (qPCR) analysis revealed the presence of HearSNPV genomes in a small fraction of multinucleocapsid ODVs representing 0.47–0.88% of the genomes quantified in ODV samples, indicating that both viruses had replicated in coinfected host cells. End-point dilution assays on ODVs from cooccluded mixed-virus OBs confirmed the presence of both viruses in 41.9–55.6% of wells that were predicted to have been infected by a single ODV. A control experiment indicated that this result was unlikely to be due to the adhesion of HearSNPV ODVs to HearMNPV ODVs or accidental contamination during ODV band extraction. Therefore, the disparity between the qPCR and end-point dilution estimates of the prevalence of mixed-virus ODVs likely reflected virus-specific differences in replication efficiency in cell culture and the higher infectivity of pseudotyped ODVs that were produced in coinfected parental cells. Bioassays on H. armigera, Spodoptera frugiperda and Mamestra brassicae larvae revealed that mixed-virus OBs were capable of infecting heterologous hosts, but relative potency values largely reflected the proportion of HearMNPV present in each mixed-virus preparation. The cooccluded mixtures were unstable in serial passage; HearSNPV rapidly dominated during passage in H. armigera whereas HearMNPV rapidly dominated during passage in the heterologous hosts. We conclude that mixed-virus coocclusion technology may be useful for producing precise mixtures of viruses with host range properties suitable for the control of complexes of lepidopteran pests in particular crops, although this requires validation by field testing.

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Section: Discussionmentioning

confidence: 99%

Coocclusion of Helicoverpa armigera Single Nucleopolyhedrovirus (HearSNPV) and Helicoverpa armigera Multiple Nucleopolyhedrovirus (HearMNPV): Pathogenicity and Stability in Homologous and Heterologous Hosts

Arrizubieta

Simón

Ricarte-Bermejo

et al. 2022

Viruses

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“…SfMNPV-6nd OBs obtained from larvae were extracted and serially diluted for absolute quantification. The qPCR for SfMNPV with specific primers for the sf32 gene was carried out in a Rotor gene 5plex HRM platform (Qiagen), according to [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…The SF-21 and Sf9 cell lines support the wild SfMNPV isolates’ replication both in suspension and in static culture [ 14 , 15 ]. One of the downsides of the serial passage of virus in insect cell culture is the occurrence of Few-Polyhedra (FP) mutants that produce low yields of occlusion bodies (OBs), which have an advantageous rate of virus replication [ 16 ] but show a low virulence of SfMNPV produced in SF-21 cells [ 17 ]. At the laboratory scale, the in vitro production of SfMNPV is considered commercially viable when it reaches 300 Occlusion Bodies (OBs)/cell [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Bioreactor Production Process of Spodoptera frugiperda multiple nucleopolyhedrovirus Biopesticide

Klafke,

Sanches,

Sihler

et al. 2023

Pathogens

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Spodoptera frugiperda (fall armyworm) is one of the most important maize pests in the world and the baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells and virus production. The Bioreactor Stirred Tank Reactor (STR) approach with glutamine-supplemented Sf-900 III serum free culture medium, combined with the MOI of 1.0, showed the best viral production performance, with a specific productivity above 300 occlusion bodies (OBs)/cell and volumetric productivity of 9.0 × 1011 OBs/L.

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“…Os bioinsumos são produtos à base de microrganismos, podendo ser fungos, bactérias ou vírus, utilizados na prevenção ou controle de insetos-praga e/ou fitopatógenos, estes produtos são desenvolvidos visando a eficácia, viabilidade e segurança para o manejo em variados sistemas de cultivo (IWANICKI et al, 2022). Os produtos à base de vírus apresentam alta seletividade, especificidade e longevidade de ação no controle de lagartas (SANCHES et al, 2021).…”

Section: Produtos Registrados No Brasilunclassified

Smart City E Smart Grid No Brasil: O Papel Do Poder Público Na Produção De Cidades Sustentáveis

Brasil,

Silva,

Gouveia

et al. 2023

Meio Ambiente: Agricultura, Desenvolvimento E Sustentabilidade 2

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Editora Direitos para esta edição cedidos à Atena Editora pelos autores. Open access publication by Atena Editora Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição-Não-Comercial-NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam necessariamente a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterála de nenhuma forma ou utilizá-la para fins comerciais.Todos os manuscritos foram previamente submetidos à avaliação cega pelos pares, membros do Conselho Editorial desta Editora, tendo sido aprovados para a publicação com base em critérios de neutralidade e imparcialidade acadêmica.A Atena Editora é comprometida em garantir a integridade editorial em todas as etapas do processo de publicação, evitando plágio, dados ou resultados fraudulentos e impedindo que interesses financeiros comprometam os padrões éticos da publicação. Situações suspeitas de má conduta científica serão investigadas sob o mais alto padrão de rigor acadêmico e ético. Conselho Editorial Ciências Agrárias e Multidisciplinar

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Successful co-infection of two different baculovirus species in the same cell line reveals a potential strategy for large in vitro production

Cited by 4 publications

References 31 publications

Coocclusion of Helicoverpa armigera Single Nucleopolyhedrovirus (HearSNPV) and Helicoverpa armigera Multiple Nucleopolyhedrovirus (HearMNPV): Pathogenicity and Stability in Homologous and Heterologous Hosts

Coocclusion of Helicoverpa armigera Single Nucleopolyhedrovirus (HearSNPV) and Helicoverpa armigera Multiple Nucleopolyhedrovirus (HearMNPV): Pathogenicity and Stability in Homologous and Heterologous Hosts

Bioreactor Production Process of Spodoptera frugiperda multiple nucleopolyhedrovirus Biopesticide

Smart City E Smart Grid No Brasil: O Papel Do Poder Público Na Produção De Cidades Sustentáveis

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