Plants, as sessile organisms, adapt to different stressful conditions, such as drought, salinity, extreme temperatures, and nutrient deficiency, via plastic developmental and growth responses. Depending on the intensity and the developmental phase in which it is imposed, a stress condition may lead to a broad range of responses at the morphological, physiological, biochemical, and molecular levels. Transcription factors are key components of regulatory networks that integrate environmental cues and concert responses at the cellular level, including those that imply a stressful condition. Despite the fact that several studies have started to identify various members of the MADS-box gene family as important molecular components involved in different types of stress responses, we still lack an integrated view of their role in these processes. In this review, we analyze the function and regulation of MADS-box gene family members in response to drought, salt, cold, heat, and oxidative stress conditions in different developmental processes of several plants. In addition, we suggest that MADS-box genes are key components of gene regulatory networks involved in plant responses to stress and plant developmental plasticity in response to seasonal changes in environmental conditions.

The insecticidal properties of HearSNPV-SP1 mean that this strain is likely to prove useful as the basis for a biological insecticide for control of Helicoverpa armigera in Spain.

The genotypic diversity of two Spanish isolates of Helicoverpa armigera single nucleopolyhedrovirus (HearSNPV) was evaluated with the aim of identifying mixtures of genotypes with improved insecticidal characteristics for control of the cotton bollworm. Two genotypic variants, HearSP1A and HearSP1B, were cloned in vitro from the most pathogenic wild-type isolate of the Iberian Peninsula, HearSNPV-SP1 (HearSP1-wt). Similarly, six genotypic variants (HearLB1 to -6) were obtained by endpoint dilution from larvae collected from cotton crops in southern Spain that died from virus disease during laboratory rearing. Variants differed significantly in their insecticidal properties, pathogenicity, speed of kill, and occlusion body (OB) production (OBs/larva). HearSP1B was ϳ3-fold more pathogenic than HearSP1-wt and the other variants. HearLB1, HearLB2, HeaLB5, and HearLB6 were the fastest-killing variants. Moreover, although highly virulent, HearLB1, HearLB4, and HearLB5 produced more OBs/ larva than did the other variants. The co-occluded HearSP1B:LB6 mixture at a 1:1 proportion was 1.7-to 2.8-fold more pathogenic than any single variant and other mixtures tested and also killed larvae as fast as the most virulent genotypes. Serial passage resulted in modified proportions of the component variants of the HearSP1B:LB6 co-occluded mixture, suggesting that transmissibility could be further improved by this process. We conclude that the improved insecticidal phenotype of the HearSP1B:LB6 co-occluded mixture underlines the utility of the genotypic variant dissection and reassociation approach for the development of effective virus-based insecticides.A lphabaculoviruses (Baculoviridae) are lepidopteran-specific nucleopolyhedroviruses (NPVs) that have been used successfully as biological control agents against several agricultural and forest pests (1). These viruses are characterized by high intraspecific heterogeneity not only between isolates from different geographic regions (2, 3) but also within single isolates that often comprise mixtures of several genotypes present in different proportions (4-8). The molecular heterogeneity of genotypes is often associated with phenotypic differences in pathogenicity, speed of kill, and virus production (8, 9), which are traits of practical importance for the use of these viruses as biological insecticides (10). Infections involving mixtures of genotypes can result in positive (11-14) or negative (15-18) effects on a number of these traits.As natural alphabaculovirus populations in virus-killed insects comprise mixtures of genotypes, the genotypic interactions that modulate the insecticidal characteristics have attracted the attention of insect pathologists (8,(19)(20)(21) and have been used to develop novel products based on unique user-defined genotypic combinations in specific user-defined proportions that result in improved insecticidal properties of the final mixture (22,23). This has resulted in the generation of a novel paradigm for the development of baculovirus-based insectici...