The water birds are inseparable part of the environment and ecosystems. This group of birds has got an important role in the functioning of aquatic ecosystems make them sensitive indicators of the health of these environments. The main aim of the study was to find out the attitudes toward and knowledge about water birds among lower secondary school pupils. The partial aims were to find out the influence of demographic variables like gender, grade, residence and the ownership of pet on the attitudes toward and knowledge about water birds. The sample size consisted of 340 lower secondary school pupils from Czech Republic. The obtained data were analyzed by the methods of descriptive (mean score), inferential (analysis of variance) and also multidimensional statistics (factor analysis). The gender and grade level of pupils had got significant level on knowledge. All observed variables had got significant level on the attitudes toward water birds. And the relationship between knowledge and attitudes was negative. The implications to educational practice are discussed. Keywords: attitudes toward water birds; knowledge about water birds; lower secondary school pupils; water birds of the health of environments. The influence of human activities can lead to the declining of the water birds' occurrence and it can have got the influence on quality of environment. The effects of different factors were examined in some studies (e.g. Hockin et al. 1992). It is obvious, water birds are very important for the nature, for the people, so it is important to examine pupils' knowledge and attitudes toward this group of animals. Because it is important to protect water birds for the retention of the quality of our life. THEORETICAL BACKGROUND The studies, which are focused on the attitudes toward and knowledge about water birds, are very rare. This group of animals is examined only as a part of bigger research. Authors Dauotopoulos & Pyrovetsi (1990) focused on the conservation attitudes of fishermen. Authors identified, the conflict was towards the fish-eating birds, particularly cormorants preying heavily on the fish ponds they have constructed. Problems with cormorants are according many authors exaggerated (e.g. Duffy 1995). According to author there is little evidence to suggest that cormorants seriously deplete commercial food sources. Klein (1993) warned on the disturbing behavior of the recreationists on the water animals. Author noticed, that the people, who spoke with refuge staff early during their visit caused the least disturbance. Authors highlighted the importance of educational programs. Educational programs, coupled with the use of observation blinds or guided tours, could help reduce bird disturbance. The similar study is possible to find out in the region of Australia, where authors (Glover et al. 2011) were focused on the disruptive behavior of the people on shorebirds in Australia. Respondents from the research reported an overall positive attitude to shorebird conservation. The similar results are possible to fi...