Subtyping of<i>Cryptosporidium cuniculus</i>and genotyping of<i>Enterocytozoon bieneusi</i>in rabbits in two farms in Heilongjiang Province, China

Yang, Ziyin; Zhao, Wei; Shen, Yujuan; Zhang, Weizhe; Ying, Shi; Ren, Guangxu; Yang, Di; Ling, Hong; Yang, Fengkun; Liu, Aiqin; Cao, Jianping

doi:10.1051/parasite/2016063

Cited by 28 publications

(33 citation statements)

References 63 publications

(84 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The prevalence of E. bieneusi in the samples in the current study was 2.8% (9/321), which is in accordance with previous studies conducted in China and Spain (Galvan‐Diaz ; Yang et al. ; Zhang et al. ).…”

Section: Discussionsupporting

confidence: 92%

“…The prevalence of E. bieneusi in the samples in the current study was 2.8% (9/321), which is in accordance with previous studies conducted in China and Spain (Galvan-Diaz 2014;Yang et al 2016;Zhang et al 2016). The nine E. bieneusi isolates in this study belonged to three different genotypes, and phylogenetic analysis revealed that genotype J and BEB8 isolates belonged to group 2, while the Type IV isolates were classed as zoonotic group 1 (Fiuza et al 2016;Li et al 2015).…”

Section: Discussionsupporting

confidence: 92%

“…2014b), but lower than that in Heilongjiang Province (11.2%, 24/215) (Yang et al. ). Rex rabbits appeared to be more susceptible to Cryptosporidium infection than domestic rabbits, and rabbits < 3 mo of age showed the highest infection rate (6.8%, 10/148), which is also consistent with previous reports (Shi et al.…”

Section: Discussionmentioning

confidence: 94%

“…However, there have also been some sporadic cases of human infections caused by Vb family members, and reports of Va strains infecting rabbits (Yang et al. ). In the present study, the five typeable C. cuniculus isolates were identified as subtype VbA24, which has been associated with massive mortality rates in farmed rabbits in Poland (Kaupke et al.…”

Section: Discussionmentioning

confidence: 99%

“…Based on sequencing analysis of the ribosomal internal transcribed spacer (ITS) region, E. bieneusi strains have been divided into eight groups (Groups 1–8), with zoonotic genotype Group 1 strains identified in rabbits in northeastern China (Yang et al. ; Zhang et al. ).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Rabbits in Xinjiang, China

Zhang

Jing

et al. 2018

J Eukaryotic Microbiology

View full text Add to dashboard Cite

A total of 321 rabbit fecal samples were collected from 10 farms in the Xinjiang Uyghur Autonomous Region, China. The prevalence of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in the samples was 3.4% (11/321), 1.9% (6/321), and 2.8% (9/321), respectively. Small subunit ribosomal RNA (SSU rRNA) gene sequence analysis identified all 11 Cryptosporidium-positive samples as C. cuniculus. Further subtyping based on the 60-kDaglycoprotein locus (gp60) identified five of the C. cuniculus isolates as subtype VbA24. G. duodenalis genotypes were determined by multilocus sequence typing of the SSU rRNA, triosephosphate isomerase, β-giardin and glutamate dehydrogenase loci, which confirmed that six G. duodenalis isolates belonged to subtype BIV of assemblage B. Analysis of the internal transcribed spacer region, showed that five, three, and one E. bieneusi isolates belonged to genotypes J, BEB8, and Type IV, respectively. These results suggest that Cryptosporidium spp., G. duodenalis, and E. bieneusi isolates from rabbits in China have zoonotic potential.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Rabbits in Xinjiang, China

Zhang

Jing

et al. 2018

J Eukaryotic Microbiology

View full text Add to dashboard Cite

show abstract

Molecular characterization of Enterocytozoon bieneusi isolates in laboratory macaques in north China: zoonotic concerns

Yang

Lin

et al. 2017

Parasitol Res

View full text Add to dashboard Cite

The significance of wild and zoo nonhuman primates (NHPs) as potential sources of human Enterocytozoon bieneusi infections has been increasingly appreciated, while the role of laboratory NHPs in zoonotic transmission of microsporidiosis remains elusive. In this study, the infection rate, genetic characteristic, and zoonotic potential of E. bieneusi were investigated for 205 laboratory macaques in Beijing, north China. The parasite was identified in 37 (18.0%) animals by nested PCR and sequencing of the ribosomal internal transcribed spacer (ITS), with an infection rate of 25.6% in Macaca fascicularis (34/133) and 4.2% in Macaca mulatta (3/72). The differences in infection rate between the two species of macaques and between young macaques aged ≤ 5 years (29.6%, 32/108) and adults aged > 5 years (5.2%, 5/97) were significant (p < 0.01). Analysis of the ITS sequence polymorphisms recognized eight known genotypes (CC4, CM1, CM2, D, Peru8, Peru11, Type IV, and WL21) and two new genotypes (named as CMB1 and CMB2), with well-known human-pathogenic genotypes (D, Peru8, Peru11, and Type IV) most frequently detected. The rest genotypes (CC4, CM1, CM2, WL21, CMB1, and CMB2) were clustered into zoonotic group 1 in phylogenetic analysis. The high diversity and widespread presence of the human-pathogenic or group 1 E. bieneusi genotypes in laboratory NHPs, notably M. fascicularis and the young animals, suggest potential of zoonotic transmission. These findings imply that laboratory rhesus macaques could be significant reservoirs for human microsporidiosis.

show abstract

Enterocytozoon bieneusi genotypes in Tibetan sheep and yaks

Zhang

Cai

et al. 2018

Parasitol Res

View full text Add to dashboard Cite

Few studies have been conducted on the distribution of Enterocytozoon bieneusi genotypes in Tibetan sheep and yaks, which live outdoors in extreme climate with high altitude. In this study, fecal specimens from 312 Tibetan sheep and 554 yaks in Qinghai, China, were collected and examined for E. bieneusi by PCR-sequence analysis of the ribosomal internal transcribed spacer. Among them, 73 (23.4%) specimens from Tibetan sheep and 40 (7.2%) from yaks were positive for E. bieneusi. There were eight E. bieneusi genotypes in Tibetan sheep, including three known ones (BEB6, COS-I, and NESH5) and five novel ones (named as CHS13-CHS17). Similarly, seven E. bieneusi genotypes were found in yaks, including five known ones (J, BEB4, BEB6, COS-I, and NESH5) and two novel ones (named as CHN13 and CHN14). Most of the E. bieneusi genotypes and all frequent ones identified in the study belonged to group 2. One new subgroup of genotypes was identified within group 1. The distribution of E. bieneusi genotypes was different between Tibetan sheep and yaks, with BEB6 as the dominant one (42.5%) in Tibetan sheep and J as the dominant one (47.5%) in yaks. These data support the occurrence of host adaptation among E. bieneusi genotypes within group 2.

show abstract

Subtyping ofCryptosporidium cuniculusand genotyping ofEnterocytozoon bieneusiin rabbits in two farms in Heilongjiang Province, China

Cited by 28 publications

References 63 publications

Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Rabbits in Xinjiang, China

Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Rabbits in Xinjiang, China

Molecular characterization of Enterocytozoon bieneusi isolates in laboratory macaques in north China: zoonotic concerns

Enterocytozoon bieneusi genotypes in Tibetan sheep and yaks

Contact Info

Product

Resources

About