Molecular characterization of Enterocytozoon bieneusi isolates in laboratory macaques in north China: zoonotic concerns

Yang, Hang; Lin, Yongchao; Li, Yijing; Song, Mingxin; Lu, Yixin; Li, Wei

doi:10.1007/s00436-017-5603-y

Cited by 21 publications

(13 citation statements)

References 38 publications

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“…In the present study, the most prevalent genotype was D which has also been found in captive non-human primates, domestic rabbits, captive foxes, captive raccoon dogs, pet chinchillas, and many other captive wildlife ( Karim et al, 2014a ; Du et al, 2015 ; Qi et al, 2015 ; Yang et al, 2015 , 2016 , 2017 ; Zhao et al, 2015 ; Li et al, 2016b ; Xu et al, 2016 ; Zhang et al, 2016a , b ; Yu et al, 2017 ). In addition, the second most prevalent genotype was EbpC which was also more frequently found in some captive animals (captive foxes and captive non-human primates) ( Karim et al, 2014a ; Zhao et al, 2015 ; Yang et al, 2017 ); however, Peru11 was only found in captive non-human primates in China ( Karim et al, 2014a ; Yang et al, 2017 ). These findings also suggested that these genotypes of E. bieneusi might transmission among these captive animals.…”

Section: Discussionsupporting

confidence: 64%

Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health Concern

Zhang

Jiang

et al. 2018

Front. Microbiol.

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Enterocytozoon bieneusi is the most important causative agent of microsporidiosis and can infect almost all vertebrate and invertebrate hosts, including minks (Neovison vison). In the present study, a total of 298 feces samples (including 79 from Heilongjiang province, 31 from Hebei province, 67 from Jilin province, 90 from Liaoning province, and 31 from Shandong province, Northern China) were examined by nested PCR amplification of the internal transcribed spacer (ITS) region of the rRNA gene. The overall prevalence of E. bieneusi in minks was 10.1%, with 10.5% in Jilin province, 32.3% in Hebei province, 8.9% in Liaoning province, 0% in Shandong province, and 6.3% in Heilongjiang province. Furthermore, multiple logistic regression analysis revealed that region was only risk factors associated with E. bieneusi infection in the investigated minks. Five E. bieneusi ITS genotypes (three known genotypes, namely D, Peru11, and EbpC; two novel genotypes, namely, NCM-1 and NCM-2) were found in the current study. Importantly, genotypes D, Peru11 and EbpC, previously identified in humans, were also found in minks, which suggested that minks are the potential sources of human microsporidiosis. To the best of our knowledge, this is the first report of E. bieneusi infection in minks worldwide. The results of the present survey have implications for the controlling E. bieneusi infection in minks, other animals and humans.

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Section: Discussionsupporting

confidence: 64%

Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health Concern

Zhang

Jiang

et al. 2018

Front. Microbiol.

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“…Since the detection of E. bieneusi in AIDS patients in 1985 for the first time, this pathogen has been found in other immunocompromised/immunodeficient individuals, such as organ transplant recipients, children, and lifethreatening and persistent diarrhea often occur in these populations [2][3][4][5][6][7]. It has been recently identified in several species of non-human primates (NHPs) in China and elsewhere [8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…Among them, ITS genotypes in Group 1 and Group 2 have been found in a broad range of hosts including humans and are probably responsible for most zoonotic or cross-species E. bieneusi infections whereas host adaptation seems to be more common in genotypes of Groups 3 to 11 according to a recent review of E. bieneusi [6]. To date, more than 100 E. bieneusi genotypes have been detected in NHPs, mostly belonging to Group 1 [6,9,10,13,14,[17][18][19][20][21]. Among them, Type IV and Peru8 are common ITS genotypes in humans and various animals in many countries, including China [22].…”

Section: Introductionmentioning

confidence: 99%

Multilocus sequence typing of Enterocytozoon bieneusi in crab-eating macaques (Macaca fascicularis) in Hainan, China

Chen

Guo

et al. 2020

Parasites Vectors

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Background: Enterocytozoon bieneusi is one of common intestinal pathogens in humans and animals including nonhuman primates (NHPs). Many zoonotic pathogens including E. bieneusi have been found in these animals. However, there are few studies on the population structure of E. bieneusi in NHPs. To infer the gene diversity and population genetics of E. bieneusi, we selected 88 E. bieneusi-positive samples from crab-eating macaques for multilocus characterizations in this study.Methods: The E. bieneusi isolates examined belonged to three common genotypes with different host ranges by sequence analysis of the ribosomal internal transcribed spacer (ITS): Type IV (n = 44), Macaque3 (n = 24) and Peru8 (n = 20). They were further characterized by sequence analysis at four microsatellite and minisatellite loci (MS1, MS3, MS4 and MS7). DnaSP, Arlequin and LIAN were used to analyze the sequence data together with those from the ITS locus to infer the population genetics. Subpopulation structure was inferred using phylogenetic and STRU CTU RE analyses.Results: Seventy-two (81.8%), 71 (80.7%), 76 (86.4%) and 79 (89.8%) samples were amplified and sequenced successfully at the MS1, MS3, MS4 and MS7 loci, respectively, with 53 having sequence data at all five MLST loci including ITS. Altogether, 33 multilocus genotypes (MLGs) were produced based on concatenated sequences from the 53 samples. In phylogenetic analyses of sequences and allelic data, four major subpopulations (SPs) were observed with different ITS genotypes in each of them: Type IV and Peru8 in SP1 and SP2; Type IV, Macaque3 and Peru8 in SP3; and Type IV and Macaque3 in SP4. SP3 and SP4 were phylogenetically related and might be NHP-specific based on the fact that Macaque3 is mostly found in NHPs. A strong linkage disequilibrium (LD) was observed among the multilocus sequences and allelic data. Conclusions:The significant LD in the multilocus sequence analysis indicated the presence of an overall clonal population structure of E. bieneusi in crab-eating macaques. The inconsistent segregation of MLGs among ITS genotypes © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article' Publisher's NoteSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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“…In the previous reports, genotype D (synonymous with genotypes PigEBITS9, WL8, Peru9, CEbC, PTEb VI) were identified in many mammals including human, reptile and birds ( Da Cunha et al, 2017 ; Huang et al, 2019 ). The reports of genotype CM1 (synonymous with genotype Macaque3) was less, it was only found in NHPs and dog at present ( Karim et al, 2014a , 2014b , 2015 ; Yang et al, 2017 ).…”

Section: Discussionmentioning

confidence: 87%

“…However, higher infection rate of 31.1% (33/106) was reported in six zoos in eastern China ( Karim et al, 2015 ). In Beijing Rhesus macaque laboratory, the infection rate of E. bieneusi in cynomolgus monkeys was 25.6% while in Rhesus macaques was only 4.2% (3/72) ( Yang et al, 2017 ). The reasons for the differences in infection rates may be related to the environmental conditions of different populations, the health of the animals at the time of sampling and the overall sample size.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological investigation and genotypes of Enterocytozoon bieneusi in 11 captive Rhesus macaque populations

Xie

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

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Enterocytozoon bieneusi is an obligate intracellular parasite and the most common pathogen of microsporidiosis in humans and animals. In this study, a total of 198 fecal samples were collected from 11 captive populations of Rhesus macaque in Chinese zoos, to investigate the prevalence and analyze the zoonotic potential of E. bieneusi by genotype of the Internal Transcribed Spacer (ITS) gene on the rRNA of E. bieneusi via nested PCR. Results showed that the average infection rate of E. bieneusi in the 11 populations was 13.6%, and the highest infection rate was 56.5% in the population of Xinjiang Tianshan Zoo. Seven genotypes were identified including 2 known genotypes (D and CM1) and 5 novel genotypes (Mul1, Mul2, Mul3, Mul4 and Mul5). Phylogenetic analysis revealed that the novel genotypes Mul2, Mul3, Mul4 and Mul5 belonged to Group 1 showed the zoonotic potential．These findings extend the distribution of E. bieneusi genotypes and provide baseline data for controlling E. bieneusi infection.

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Molecular characterization of Enterocytozoon bieneusi isolates in laboratory macaques in north China: zoonotic concerns

Cited by 21 publications

References 38 publications

Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health Concern

Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health Concern

Multilocus sequence typing of Enterocytozoon bieneusi in crab-eating macaques (Macaca fascicularis) in Hainan, China

Epidemiological investigation and genotypes of Enterocytozoon bieneusi in 11 captive Rhesus macaque populations

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