Subtype analysis of Blastocystis spp. isolates from symptomatic and asymptomatic patients in Suez Canal University Hospitals, Ismailia, Egypt

Abaza, Sherif; Rayan, Hanan Z.; Soliman, Rabie; Nemr, Nader; Mokhtar, Amira B.

doi:10.4103/1687-7942.139691

Cited by 18 publications

(25 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The absence of parasites in the small intestine in all infected mice is consistent with previous reports [6,28,29,31,43] that detected Blastocystis spp. organisms only in the cecum and colon.…”

Section: Discussionsupporting

confidence: 92%

“…As regards the naked eye appearance of mice intestine, cecum and colon of mice in GI inoculated with CRC isolates and mice in GII inoculated with non-CRC symptomatic isolates, showed some redness and bloating in contrast to those inoculated by asymptomatic isolates that showed normal gross morphology. This was similar to the results observed by Abaza et al [6] who noticed redness and bloating in cecum and colon of rats infected by symptomatic isolates, but not in those inoculated by asymptomatic isolates and isolates derived from IBS patients, suggesting differences in pathogenicity of human' isolates derived from persons with different clinical presentations.…”

Section: Discussionsupporting

confidence: 91%

“…Positive stools examinations of mice began from 4 to 6 days PI in the three groups. Earlier diagnosis of infection of 2 to 5 days [28,44] in mice, and 3 to 4 days [6] in rats were reported depending on the inoculum size.…”

Section: Discussionmentioning

confidence: 99%

“…Although the amoeboid form is the proposed pathogenic form of Blastocystis spp. [46][47][48] , yet in studies describing the histopathology of experimental blastocystosis, only the vacuolar forms were reported to invade the wall of the intestine [6,20,28,31,32,45,49,50] . Similarly, in the present work in sections of the large intestine of mice in GI (4/7 mice) and GII (2/6 mice), only the lamina propria appeared to be invaded by the vacuolar forms.…”

Section: Discussionmentioning

confidence: 99%

“…Different degrees of pathological changes were reported among mice and rats experimentally infected by symptomatic human isolates compared with asymptomatic isolates [6,31,45,49,50] . A mild degree of inflammation with few inflammatory cells infiltration and aggregates of lymphocytes was previously reported in rats, with the parasite seen at the luminal aspect of the colon when the animals were inoculated by asymptomatic isolates [6,31] . Similarly, edematous lamina propria with mild inflammatory cellular infiltration especially neutrophils, eosinophils and lymphocytes were reported in experimentally infected mice [29] .…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Preneoplastic proliferative changes induced by experimental blastocystosis

Ahmed

Habib

Saad

et al. 2019

Parasitologists United Journal

View full text Add to dashboard Cite

Blastocystis spp. are the most common eukaryotic protist encountered in the gut of both humans and many animals [1]. The organism has been diagnosed in both patients with non-specific gastrointestinal symptoms such as nausea, vomiting, diarrhea, abdominal pain, flatulence, and in asymptomatic individuals [2-4]. Infection has been associated with a variety of diseases such as irritable bowel syndrome (IBS) [5-9] , non-specific colitis, chronic inflammatory bowel disease (IBD) including Crohn's disease and ulcerous colitis [10,11] , and with CRC [12-14]. It has been diagnosed in association with non-gastrointestinal disorders such as skin rash and chronic urticaria [15-18] , as well as Hashimoto's thyroiditis [19]. Because of these discrepancies in associations, and despite the application of recent advances in immunological and molecular methods to Blastocystis spp. research, the pathogenicity of Blastocystis spp.is still controversial and inconclusive [11,20]. The pathogenic potential of Blastocystis spp. has been investigated by in vitro as well as in vivo experimental studies [21]. In vitro studies demonstrated the induction of apoptosis in cell lines [22,23] in contrast to the proliferation of human colonic cancer cell line, HCT116, when incubated with Blastocystis spp. organisms [24,25]. In vivo studies demonstrated pre-neoplastic changes with the formation of aberrant crypt foci in the colon of Blastocystis spp. experimentally infected rats [6]. These studies led to suspicions of Blastocystis spp. as a cause of cancer colon. However, until recently this view remains to be under investigation [20]. The pathological lesions produced in animals by Blastocystis spp. isolates derived from asymptomatic and symptomatic infected humans with gastrointestinal manifestations [27-30] , and from patients with IBS [7,31,32] have been studied and compared. The present study aims to investigate the pathology induced in the gut of mice inoculated with Blastocystis spp. isolates derived from patients with and without CRC to discover the possible oncogenic potential of the parasite.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Preneoplastic proliferative changes induced by experimental blastocystosis

Ahmed

Habib

Saad

et al. 2019

Parasitologists United Journal

View full text Add to dashboard Cite

show abstract

Levels of Genetic Variants Among Symptomatic Blastocystis Subtypes and their Relationship to Mucosal Immune Surveillance in the Precancerous Colons of Experimentally Infected Rats

et al. 2022

View full text Add to dashboard Cite

Purpose The relationship between the genetic diversity of Blastocystis and immune surveillance in precancerous colons with blastocystosis is still under investigation. This study aimed to identify the genetic Blastocystis variants among 54 symptomatic human isolates and their relationship to mucosal immune surveillance in the precancerous polyps of experimentally infected rats. Methods Polymerase chain reaction and high-resolution melting (PCR/HRM) curves discriminated human symptomatic Blastocystis isolates into subtypes (STs)/intrasubtypes, which were orally administered to rats to induce experimental infection. Then, the mucosal immune responses of the infected colons were evaluated in relation to polyp formation through immunostaining to identify mucus MUC2 and determine mucosal immune cell (goblet, lymphocyte and mast) counts, secretory IgA levels and parasitic intestinal invasion. Results ST1, ST3, and ST4 were found in 18.5% (10/54), 54.7% (29/54), and 27.8% (15/54) of the samples, respectively. Then, the HRM curve discriminated ST3 into the wild, mutant, and heterozygous [17/54 (31.5%), 5/54 (9.3%), and 7/54 (12.9%)] intrasubtypes. ST1 and ST4 had no genetic variations. Precancerous polyps were detected in the colons of 40.5% of the infected rats. ST1 constituted 14.7% of these cases, while the wild, mutant, and heterozygous intrasubtypes of ST3 showed polyps in 12.9%, 5.5%, and 5.5% of cases, respectively. Only 1.9% of the polyps were related to ST4. MUC2 showed weak immunostaining in 44.5% of the infected colons, and 38.9% were polyp inducers. Low goblet cell numbers and high interepithelial lymphocyte counts were significantly associated with polyp formation, particularly with ST1 and wild ST3. Among the polyp inducers, high numbers of mast cells were detected in wild ST3 and ST4, while a low number was found with heterozygous ST3. The level of secretory IgA was low in polyp-inducing STs. Most of the results were statistically significant. Conclusion Immunosurveillance showed a potential relationship between ST1 and the ST3 intrasubtypes and precancerous polyps. This relationship may provide insight into the prevention and/or development of new immunotherapeutic strategies to combat colorectal cancer.

show abstract

Surface ultrastructure, protein profile and zymography of Blastocystis species isolated from patients with colorectal carcinoma

et al. 2019

View full text Add to dashboard Cite

Oncogenic potential of Blastocystis species have been predicted on reporting the enhanced proliferation of human colorectal cancer cells by the parasite solubilized antigen in vitro, and the enhanced drug-induced carcinogenesis by infection in vivo. The present study is the first to investigate some phenotypic characters, namely the surface ultrastructure, protein profiles and protease activity of Blastocystis sp. isolated from three different clinical groups: colorectal carcinoma (CRC) patients, symptomatic and asymptomatic infected persons. Under SEM, all CRC Blastocystis sp. isolates had a very rough intensely folded surface in comparison to the less rough and completely smooth surface of all symptomatic and asymptomatic Blastocystis sp. Non-CRC isolates, respectively. Under reducing conditions, the sodium dodecyl sulfate-polyacrylamide gel electrophoresis had shown a significant presence of 2 protein bands of 230 and 32 KDa in 42.9% of Blastocystis sp. CRC isolates with their complete absence from Non-CRC isolates. While using non-reducing condition with the incorporation of gelatin in the gel to study the protease activity of the parasite, no significant difference existed between isolates of the three groups. In conclusion, the significant difference in surface ultrastructure and in protein profiles exists between Blastocystis sp. of CRC and Non-CRC isolates. These differences might be either secondary to the altered gut environment in the presence of CRC or are indicators of a different pathogenic potential of the parasite isolates inducing malignancy.

show abstract

Subtype analysis of Blastocystis spp. isolates from symptomatic and asymptomatic patients in Suez Canal University Hospitals, Ismailia, Egypt

Cited by 18 publications

References 0 publications

Preneoplastic proliferative changes induced by experimental blastocystosis

Preneoplastic proliferative changes induced by experimental blastocystosis

Levels of Genetic Variants Among Symptomatic Blastocystis Subtypes and their Relationship to Mucosal Immune Surveillance in the Precancerous Colons of Experimentally Infected Rats

Surface ultrastructure, protein profile and zymography of Blastocystis species isolated from patients with colorectal carcinoma

Contact Info

Product

Resources

About