Subtribe‐specific monoclonal antibodies to Lolium perenne

Abstract

Monoclonal antibodies that are functionally specific to only two grasses can be produced and used to discriminate between related grass species.

IgE cross-reactivity between Lolium multiflorum and commercial grass pollen allergen extracts in Brazilian patients with pollinosis

“…In contrast, the GI extract, which showed less than 50% inhibition for Lm immunodominant components, presents in its composition grass pollen tribes (Triticeae and Aveneae) phylogenetically different from L. multiflorum (Poeae). Altogether, these results are consistent with inhibition radioimmunoassay and ELISA studies, using crude extracts of grass pollen of the family Poaceae that showed strong cross-reactivity between their members (1,10,36). Further inhibition experiments should be conducted with purified or recombinant major allergens to confirm the grouping of the individual allergens.…”

“…Studies of the characterization of the major allergens from L. multiflorum are limited, in contrast to a number of studies concerning the characterization, allergenic sensitization and cross-reactivity of pollen allergens from L. perenne and other grasses of the same family of L. multiflorum (1,2,10). In the present study, high homologous inhibition with low IC 50 was observed in ELISA for the detection of IgE in response to the Lm extract, reflecting the specificity of the reaction.…”

“…Furthermore, an increasing number of grass pollen species have been recognized as important allergen sources (1)(2)(3). Among the different types of allergenic grass pollen species, Poaceae family plants such as Lolium perenne, Poa pratensis and Phleum pratense are the major sources of grass pollen allergens due to their broad distribution in certain geographic areas and high capacity of pollen production (4,5).…”