The Halogen Assay - A New Technique for Measuring Airborne Allergen

Tovey, Euan R.; Lucca, Sandra D. De; Poulos, L; O'Meara, T.J.

doi:10.1007/978-1-59745-366-0_19

Cited by 8 publications

(7 citation statements)

References 23 publications

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“…Unfortunately, the definition obtained with the image acquisition hardware and software was not sufficient to isolate the particles from the haloes in cases in which the haloes obscured the particle of origin, precluding us from conducting analysis similar to De Lucca et al Comparing the haloes’ integrated densities to that of haloes of known microdotted concentrations of allergen may have provided a better way to examine the relation of allergen concentration carried on each immunostained particles. 35 Nevertheless, it remains to be determined whether the consistent integrated densities seen before, during, and after disturbance in this study suggest that individuals may be exposed to airborne particles with similar allergen-carrying potentials in different size fractions with or without particle disturbance.…”

Section: Discussionmentioning

confidence: 79%

Performance of the halogen immunoassay to assess airborne mouse allergen-containing particles in a laboratory animal facility

Rivera-Mariani

Matsui

Breysse

2012

J Expo Sci Environ Epidemiol

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Airborne mouse allergen is a risk factor for respiratory diseases. Conventional assessment techniques provide mass-based exposure estimates that may not capture completely the inhalation risk of airborne allergen particles. In contrast to mass-based estimates, the halogen immunoassay (HIA) combines immunoblotting and microscopy to directly assess allergen-containing particles. We evaluated the HIA for the assessment of airborne mouse allergen and compared the results to the enzyme linked immunosorbent assay (ELISA). Particulate matter (PM)10 and PM2.5 samples (30 min, 4 l/m) were collected in a mouse facility before, during, and after disturbance of soiled bedding. Concentrations of Mus m 1-positive particles (haloed particles (HPs)) and intensities of the haloes were determined with the HIA. Although HPs/m3 were positively correlated with mass concentration (statistically significant only with Mus m 1 concentration on PM10), replicates of mass concentration showed higher variability than HPs/m3. After disturbance, most of the HPs were in the PM2.5 fraction. Mean haloes intensities were similar before, during, and after disturbance. The HIA was able to measure allergen-containing particles with less variability than the ELISA, detected the shift of HPs to smaller particles after disturbance, and may suggests similar halo intensity by particles detected during and after disturbance. Our findings suggest that the HIA can be used to assess indoor concentrations of mouse allergen particles and their morphological characteristics.

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Section: Discussionmentioning

confidence: 79%

Performance of the halogen immunoassay to assess airborne mouse allergen-containing particles in a laboratory animal facility

Rivera-Mariani

Matsui

Breysse

2012

J Expo Sci Environ Epidemiol

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“…An alternate immunodiagnostic platform that could be utilized to quantify pollen bioaerosols is the Halogen Immunoassay (HIA) (Tovey et al, 2008). In this indirect immunoassay, airborne pollen is collected onto a protein binding membrane (PBM) such as polyvinylidene fluoride or mixed cellulose ester.…”

Section: Alternative Methodsmentioning

confidence: 99%

“…Like traditional methods, the HIA is confounded by examiner bias associated with the microscopic identification of pollen and it is a technically challenging immunoassay. Although this approach has been utilized in clinical studies and has provided unique insights into personal exposure to a variety of bioaerosol sources (Tovey et al, 2008), the HIA would not be a practical immunoassay platform for the rapid detection of pollen collected at a monitoring site.…”

Section: Alternative Methodsmentioning

confidence: 99%

Immunologic, spectrophotometric and nucleic acid based methods for the detection and quantification of airborne pollen

Rittenour

Hamilton

Beezhold

et al. 2012

Journal of Immunological Methods

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Microscopic identification of pollen morphological phenotypes has been the traditional method used to identify and quantify pollen collected by air monitoring stations worldwide. Although this method has enabled a semi-standardized approach for the assessment of pollen exposure, limitations including labor intensiveness, required expertise, examiner bias, and the inability to differentiate species, genera, and in some cases families have limited data derived from the these stations. Recent advances in chemical, biochemical and molecular detection methods have provided standardized alternatives to this microscopic approach. In this review, we examine the applicability of alternative methodologies, in particular nucleic acid based assays involving the quantitative polymerase chain reaction, for the standardized detection of airborne pollen.

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“…It is also possible to count allergen-bearing particles with the so-called halogen immunoassay (49,50). Lateral flow immunoassays (LFIA) allow direct semiquantitative identification of allergens in a test solution or dust extract, as shown for alphaamylase (51), laboratory animal allergens (52), and house dust mite (53); LFIA are particularly useful to quickly show the presence of allergens in a specific situation.…”

Section: Allergen Analysismentioning

confidence: 99%

Monitoring of occupational and environmental aeroallergens – EAACI Position Paper

Raulf

Buters

Chapman³

et al. 2014

Allergy

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Exposure to high molecular weight sensitizers of biological origin is an important risk factor for the development of asthma and rhinitis. Most of the causal allergens have been defined based on their reactivity with IgE antibodies, and in many cases, the molecular structure and function of the allergens have been established. Significant information on allergen levels that cause sensitization and allergic symptoms for several major environmental and occupational allergens has been reported. Monitoring of high molecular weight allergens and allergen carrier particles is an important part of the management of allergic respiratory diseases and requires standardized allergen assessment methods for occupational and environmental (indoor and outdoor) allergen exposure. The aim of this EAACI task force was to review the essential points for monitoring environmental and occupational allergen exposure including sampling strategies and methods, processing of dust samples, allergen analysis, and quantification. The paper includes a summary of different methods for sampling and allergen quantification, as well as their pros and cons for various exposure settings. Recommendations are being made for different exposure scenarios.

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The Halogen Assay - A New Technique for Measuring Airborne Allergen

Cited by 8 publications

References 23 publications

Performance of the halogen immunoassay to assess airborne mouse allergen-containing particles in a laboratory animal facility

Performance of the halogen immunoassay to assess airborne mouse allergen-containing particles in a laboratory animal facility

Immunologic, spectrophotometric and nucleic acid based methods for the detection and quantification of airborne pollen

Monitoring of occupational and environmental aeroallergens – EAACI Position Paper

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