2008
DOI: 10.1007/978-1-59745-366-0_19
|View full text |Cite
|
Sign up to set email alerts
|

The Halogen Assay - A New Technique for Measuring Airborne Allergen

Abstract: The Halogen assay is a new technique for measuring airborne allergen. The assay is unique in that it is capable of analyzing allergens and particles together, combining the advantages of morphological approaches and immunoassay. The Halogen assay allows direct observation of the particles that carry the allergen as well as being capable of identifying all the allergen sources an individual is exposed and sensitized to. The assay is sensitive because the extracted allergen is bound to the membrane at a high loc… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

0
7
0

Year Published

2010
2010
2016
2016

Publication Types

Select...
6
1

Relationship

0
7

Authors

Journals

citations
Cited by 8 publications
(7 citation statements)
references
References 23 publications
0
7
0
Order By: Relevance
“…Unfortunately, the definition obtained with the image acquisition hardware and software was not sufficient to isolate the particles from the haloes in cases in which the haloes obscured the particle of origin, precluding us from conducting analysis similar to De Lucca et al Comparing the haloes’ integrated densities to that of haloes of known microdotted concentrations of allergen may have provided a better way to examine the relation of allergen concentration carried on each immunostained particles. 35 Nevertheless, it remains to be determined whether the consistent integrated densities seen before, during, and after disturbance in this study suggest that individuals may be exposed to airborne particles with similar allergen-carrying potentials in different size fractions with or without particle disturbance.…”
Section: Discussionmentioning
confidence: 79%
“…Unfortunately, the definition obtained with the image acquisition hardware and software was not sufficient to isolate the particles from the haloes in cases in which the haloes obscured the particle of origin, precluding us from conducting analysis similar to De Lucca et al Comparing the haloes’ integrated densities to that of haloes of known microdotted concentrations of allergen may have provided a better way to examine the relation of allergen concentration carried on each immunostained particles. 35 Nevertheless, it remains to be determined whether the consistent integrated densities seen before, during, and after disturbance in this study suggest that individuals may be exposed to airborne particles with similar allergen-carrying potentials in different size fractions with or without particle disturbance.…”
Section: Discussionmentioning
confidence: 79%
“…An alternate immunodiagnostic platform that could be utilized to quantify pollen bioaerosols is the Halogen Immunoassay (HIA) (Tovey et al, 2008). In this indirect immunoassay, airborne pollen is collected onto a protein binding membrane (PBM) such as polyvinylidene fluoride or mixed cellulose ester.…”
Section: Alternative Methodsmentioning
confidence: 99%
“…Like traditional methods, the HIA is confounded by examiner bias associated with the microscopic identification of pollen and it is a technically challenging immunoassay. Although this approach has been utilized in clinical studies and has provided unique insights into personal exposure to a variety of bioaerosol sources (Tovey et al, 2008), the HIA would not be a practical immunoassay platform for the rapid detection of pollen collected at a monitoring site.…”
Section: Alternative Methodsmentioning
confidence: 99%
“…It is also possible to count allergen-bearing particles with the so-called halogen immunoassay (49,50). Lateral flow immunoassays (LFIA) allow direct semiquantitative identification of allergens in a test solution or dust extract, as shown for alphaamylase (51), laboratory animal allergens (52), and house dust mite (53); LFIA are particularly useful to quickly show the presence of allergens in a specific situation.…”
Section: Allergen Analysismentioning
confidence: 99%