Subtle Microbiome Manipulation Using Probiotics Reduces Antibiotic-Associated Mortality in Fish

Schmidt, Victor T.; Gomez‐Chiarri, Marta; Roy, Chelsea; Smith, Katherine F.; Amaral‐Zettler, Linda

doi:10.1128/msystems.00133-17

Cited by 65 publications

(50 citation statements)

References 51 publications

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“…and these results similar to that detected by (Lee and Wendy, 2017). On the other hand, V. alginolyticus was highly sensitive to ciprofloxacin and our results supported with that obtained by (Schmidt et al, 2017).…”

Section: Discussionsupporting

confidence: 92%

Studies on Bacterial Pathogens in Some Marine Fishes in EL-Mansoura, Egypt

Ezzat

Mahmoud

Ibrahim

et al. 2018

American Journal of Agricultural and Biological Sciences

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A total of 200 marine fish of two different species (100 Tilapia zillii and100 Mugil capito) were randomly collected from different markets, during different seasons. Fish subjected to clinical, postmortem and bacteriological studies. The common clinical signs were darkness of the skin, hemorrhage in the fins base, eyes and different parts of the body, abdominal distention, congestion in gills and increasing in mucous secretion. The Post Mortem findings showed white serous fluid in the abdominal cavity and sometimes tinged with blood and pale or congested liver, kidney and spleen. The bacteriological examination, revealed that the prevalence of bacterial pathogens among naturally infected marine fishes were A. hydrophila (52; 39.39%), V. alginolyticus (38; 28.79%), Ps. fluorescens (24; 18.18%), V. cholerae (10; 7.58%) and Ps. aeruginosa (8; 6.06%). The highest prevalence of bacterial infection was during summer (33.33%) followed by spring (24.42%), then autumn (21.97%), while the least prevalence was in the winter season (20.46%). A. hydrophila was highly pathogenic to T. zillii causing 100% mortality followed by V. alginolyticus causing (90%) mortality, then Ps. fluorescens (80%), Ps. aeruginosa (60%) while V. cholerae was non-pathogenic. The results of antibiogram revealed that ciprofloxacin and nalidixic acid were effective against A. hydrophila, while ciprofloxacin and rifampicin were effective against Ps. fluorescens. Ciprofloxacin and amikacin were effective against Ps. aeruginosa. While V. alginolyticus was highly sensitive to ciprofloxacin. High prevalence of pathogenic and resistant bacterial strains among marine fishes in Egypt, requires strict control measures from the responsible authorities.

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Section: Discussionsupporting

confidence: 92%

Studies on Bacterial Pathogens in Some Marine Fishes in EL-Mansoura, Egypt

Ezzat

Mahmoud

Ibrahim

et al. 2018

American Journal of Agricultural and Biological Sciences

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“…Traditionally, this has included, among others, the use of various probiotic microorgansims. For instance, within black molly (Poecilia sphenops) the administration of native probiotics (namely Phaeobacter inhibens and Bacillus pumilus) following antibiotic exposure led to improved disease resistance to pathogenic Vibrio species [81]. In this study, we attempted to introduce a purported probiotic Lactobacillus species (L. rhamnosus GG or LGG ® , of human origin) in conjunction with FMT, which was previously used to improve disease resistance and the immune response in other sh species [82].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic-induced alterations and repopulation dynamics of yellowtail kingfish microbiota

Legrand

Catalano

Wos‐Oxley

et al. 2020

Preprint

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Background The use of antibiotics in aquaculture is a common infection treatment and is increasing in some sectors and jurisdictions. While antibiotic treatment can negatively shift gut bacterial communities, recovery and examination of these communities in fish of commercial importance is not well documented. Examining the impacts of antibiotics on farmed fish microbiota is fundamental for improving our understanding and management of healthy farmed fish. This work assessed yellowtail kingfish ( Seriola lalandi ) skin and gut bacterial communities after an oral antibiotic combination therapy in poor performing fish that displayed signs of enteritis over an 18-day period. In an attempt to promote improved bacterial re-establishment after antibiotic treatment, faecal microbiota transplantation (FMT) was also administered via gavage or in the surrounding seawater, and its affect was evaluated over 15 days post-delivery. Results Antibiotic treatment greatly perturbed the global gut bacterial communities of poor-performing fish – an effect that lasted for up to 18 days post treatment. This perturbation was marked by a significant decrease in species diversity and evenness, as well as a concomitant increase in particular taxa like an uncultured Mycoplasmataceae sp., which persisted and dominated antibiotic-treated fish for the entire 18-day period. The skin-associated bacterial communities were also perturbed by the antibiotic treatment, notably within the first 3 days; however, this was unlike the gut, as skin microbiota appeared to shift towards a more ‘normal’ (though disparate) state after 5 days post antibiotic treatment. FMT was only able to modulate the impacts of antibiotics in some individuals for a short time period, as the magnitude of change varied substantially between individuals. Some fish maintained certain transplanted gut taxa (i.e. present in the FMT inoculum; namely various Aliivibrio related ASVs) at Day 2 post FMT, although these were lost by Day 8 post FMT. Conclusion As we observed notable, prolonged perturbations induced by antibiotics on the gut bacterial assemblages, further work is required to better understand the processes/dynamics of their re-establishment following antibiotic exposure. In this regard, procedures like FMT represent a novel approach for promoting improved microbial recovery, although their efficacy and the factors that support their success requires further investigation.

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“…To maintain sustainable aquaculture production, in last few decades, antibiotics and chemotherapeutics as traditional drugs were commonly used for infectious diseases treatment and prevention, accompanying by a risk of emergence of microbial dysbiosis, resistant pathogenic bacteria, and accumulation of antibiotics in aquaculture food products which led to food safety issues for human health (Liu, Steele, & Meng, 2017;Mo, Chen, Leung, & Leung, 2017). Therefore, the development of natural and eco-friendly methods such as non-antibiotic farming is widely accepted in Chinese aquaculture industry and globally as well (Schmidt, Gomezchiarri, Roy, Smith, & Amaralzettler, 2017).…”

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confidence: 99%

Dietary supplementation of Bacillus cereus as probiotics in Pengze crucian carp ( Carassius auratus var. Pengze): Effects on growth performance, fillet quality, serum biochemical parameters and intestinal histology

et al. 2019

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This study was aimed to evaluate the effects of dietary supplementation of Bacillus cereus as probiotics on growth, fillet quality, serum biochemical parameters and intestinal histology of Pengze crucian carp (Carassius auratus var. Pengze). Fish were fed basic diet (Control group) and three diets such as CD7, CD9 and CD11 supplemented with B. cereus at dose of 107, 109 and 1011 CFU/kg for 70 days. The results showed that supplementation of 109 CFU/kg B. cereus significantly improved the growth performance compared to that in the Control group. The textures of muscle were improved by dietary supplementation of probiotics, and the hardness, gumminess, chewiness and the resilience of fish fillet increased with increasing dose of B. cereus. Total cholesterol including high‐density lipoprotein and low‐density lipoprotein level in serum decreased correspondingly with increasing level of probiotics whereas, acid phosphatase and catalase activities increased in serum. B. cereus supplementation enhanced the immunity and antioxidant capacity via an increase in acid phosphatase, alkaline phosphatase, glutathione peroxidase, reduce glutathione, and catalase, accompanied by a significant reduction in malondialdehyde. Higher intestinal fold height was observed in CD9 group than other groups. The lowest enterocyte height was exhibited in the Control group, HE values increased with increasing dose of probiotics. Moreover, dietary inclusion of B. cereus significantly affected the digestive enzymes activities in terms of higher lipase and trypase activities. In conclusion, dietary supplementation of B. cereus could promote the growth, elevate the immunity and antioxidant status of carp, and importantly improve the texture of fish fillet.

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Subtle Microbiome Manipulation Using Probiotics Reduces Antibiotic-Associated Mortality in Fish

Cited by 65 publications

References 51 publications

Studies on Bacterial Pathogens in Some Marine Fishes in EL-Mansoura, Egypt

Studies on Bacterial Pathogens in Some Marine Fishes in EL-Mansoura, Egypt

Antibiotic-induced alterations and repopulation dynamics of yellowtail kingfish microbiota

Dietary supplementation of Bacillus cereus as probiotics in Pengze crucian carp ( Carassius auratus var. Pengze): Effects on growth performance, fillet quality, serum biochemical parameters and intestinal histology

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