Substrates and duration for conducting the safflower seed germination test

Gama, Gabriela Fernandes; Machado, Carla Gomes; Silva, Givanildo Zildo da; Moraes, Ana Laura Cruzeiro; Silva, Amalia Andreza Sousa; Silva, Ingrid Maressa Hungria de Lima e

doi:10.15361/1984-5529.2019v47n4p426-433

Cited by 6 publications

(6 citation statements)

References 8 publications

(9 reference statements)

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“…Germination test: eight repetitions of 25 seeds were sown in transparent acrylic boxes with lids (11.0 × 11.0 × 3.5 cm) containing "blotting paper" as substrate type, then the seeds were covered with one of the same paper, moistened with a volume of water (mL) equivalent to 2.5 times the mass of dry paper (g) (Brasil, 2009). The final count on the eighth day after the test installation (Gama et al, 2019) counting the number of normal, abnormal and dead seedlings, as described in the RAS (Brasil, 2009).…”

Section: Methodsmentioning

confidence: 99%

“…First count of germination: performed in conjunction with the twinning test, with a count of the number of normal seedlings on the third day after the test was installed (Gama et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

“…Cold test: 12 lots of seeds were evaluated and sown following the same methodology described above, using four sub-samples of 25 seeds for each lot. The acrylic boxes were kept in a germination chamber regulated at 10 °C for four periods (0, 3, 5, and 7 days), then placed at 25 °C, and a count of normal seedlings was performed on the third day after the test was installed (Barros et al, 1999;Brazil, 2009;Gama et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

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Cold test on the vigor determination of Carthamus tinctorius L. seeds

Coelho

Silva

et al. 2021

Agraria

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In evaluating the quality of seed lots, vigor tests are complementary to the germination test, identifying differences in their degree of deterioration. However, for safflower seeds (Carthamus tinctorius L.) there is little information regarding these tests. Thus, the objective was to adapt the cold test methodology to evaluate the vigor of safflower seeds. For that, twelve lots were initially characterized by moisture content, mass of one thousand seeds, germination and seedling emergence in the field. After characterization the samples were conditioned in transparent plastic boxes on paper moistened with water and kept at 10 °C for the periods of 0, 3, 5 and 7 days. After the conditioning periods the samples were transferred to a germination chamber at 25 ºC, with evaluation of normal seedlings on the 3rd day. The obtained data was subjected to variation analysis and when significant, compared by the Scott-Knott method at 5% probability. To verify the efficiency of the methodologies, the principal component analysis was performed. The cold testing at 10 °C for 7 days is recommended for evaluating the physiological quality of safflower seeds.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Cold test on the vigor determination of Carthamus tinctorius L. seeds

Coelho

Silva

et al. 2021

Agraria

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“…The eight replicates of 25 seeds were placed to germinate between two layers of paper (blotting paper) inside transparent plastic boxes (11.0 x 11.0 x 3.5 cm). The paper was moistened with a volume of water (mL) equivalent to 2.5 times the mass of the dry paper (g) (Brasil 2009), with final count the seeds germinated at eight days, according to Gama et al (2019).…”

Section: Germination Testmentioning

confidence: 99%

Accelerated aging test in the determination of safflower seeds vigor

Coelho

Silva

et al. 2022

Biosci. J.

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In assessing the quality of seed lots, the vigor tests are complementary to the germination test and they identify differences in the degree of deterioration of the lots. For safflower, there is little information regarding these tests. In this way, the intention of this study was to adapt the accelerated aging test methodology to assess the physiological potential of safflower seeds (Carthamus tinctorius). For this purpose, 12 seed lots were evaluated for thousand-seed weight, germination, first germination count, seedling emergence test (emergence percentage, emergence speed index, relative emergence frequency and the initial, final and mean times) and accelerated aging. For the accelerated aging test, the traditional and saline methods were used. For this, the samples were conditioned in periods of 0, 8, 16, 24, 32 and 48 hours at 42 °C. Afterwards, they were submitted to the germination test, with evaluation of normal seedlings on the 3rd day. The 12 lots were evaluated within each period, in independent experiments. The data were submitted to analysis of variance and the means were compared using the Scott-Knott clustering method at 5% probability. In the traditional accelerated aging test the periods of 16, 24, 32 and 48 hours were more efficient in differentiating the lots in vigor levels, as they stratified the lots in three classes and the time of 8 hour classified the lots in two levels of vigor. In the accelerated saline aging method the time 32 hours were more efficient since it ranked seed lots at three levels of vigor and the periods of 8, 16 and 24 hour stratified the lots in two levels. In results obtained by the principal component analysis it was verified. The variables traditional accelerated aging for 24 and 32 hours correlated with emergence in the field. Therefore, the traditional accelerated aging test at 42 °C for 24 hours are promising for evaluating the physiological quality of safflower seeds.

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“…Similarly, a study by R. C. da, Grzybowski and Panobianco (2016) with maize seeds reported that, depending on the substrate, higher and lower vigor lots could show similar performances, proving that the substrate has a greater effect on the results than the physiological quality of the seeds in germination and seedling vigor tests. However, there have also been reports that high vigor fodder pea and safflower lots were less demanding on substrate conditions than low vigor lots (Gama et al, 2019;Machado, Martins, Silva Cruz, Assis, 2019b).…”

Section: Germination and First Count Testmentioning

confidence: 99%

Envelope method for the germination test of Mombasa grass

Jeromini¹,

Botassini²,

Barros³

et al. 2021

Semina: Ciênc. Agrár.

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The Rules for Seed Analysis reports the use of a paper envelope to allow use of the germination test with small seeds but does not provide any method or use recommendation. As Mombasa grass has high quality and commercial importance, the objective of this study was to evaluate the viability of the envelope method in the germination test of Mombasa grass seeds, with the aim of achieving greater result accuracy and improvement of seed quality analysis processes in laboratories. The germination of eight lots of Mombasa grass seeds was evaluated with two sowing methods (on paper and in paper envelope). The moisture content and the first germination count were also determined. The experiment was conducted with a completely randomized design. The data were analyzed in a 2 x 8 factor scheme (sowing methods x lots), with four repetitions. The data were subjected to analysis of variance using the F test and when significant, treatment means were compared using the Tukey’s test at 5% probability. Results showed that the envelope method is efficient for the germination test of Mombasa grass seeds and can be easily used by seed analysis laboratories, as it present results equivalent or superior to those obtained using the traditional method on paper, while occupying only 2/5 of the germination chamber space used in the conventional method.

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Substrates and duration for conducting the safflower seed germination test

Cited by 6 publications

References 8 publications

Cold test on the vigor determination of Carthamus tinctorius L. seeds

Cold test on the vigor determination of Carthamus tinctorius L. seeds

Accelerated aging test in the determination of safflower seeds vigor

Envelope method for the germination test of Mombasa grass

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