Accelerated aging test in the determination of safflower seeds vigor

Abstract

In assessing the quality of seed lots, the vigor tests are complementary to the germination test and they identify differences in the degree of deterioration of the lots. For safflower, there is little information regarding these tests. In this way, the intention of this study was to adapt the accelerated aging test methodology to assess the physiological potential of safflower seeds (Carthamus tinctorius). For this purpose, 12 seed lots were evaluated for thousand-seed weight, germination, first germination count, seedling emergence test (emergence percentage, emergence speed index, relative emergence frequency and the initial, final and mean times) and accelerated aging. For the accelerated aging test, the traditional and saline methods were used. For this, the samples were conditioned in periods of 0, 8, 16, 24, 32 and 48 hours at 42 °C. Afterwards, they were submitted to the germination test, with evaluation of normal seedlings on the 3rd day. The 12 lots were evaluated within each period, in independent experiments. The data were submitted to analysis of variance and the means were compared using the Scott-Knott clustering method at 5% probability. In the traditional accelerated aging test the periods of 16, 24, 32 and 48 hours were more efficient in differentiating the lots in vigor levels, as they stratified the lots in three classes and the time of 8 hour classified the lots in two levels of vigor. In the accelerated saline aging method the time 32 hours were more efficient since it ranked seed lots at three levels of vigor and the periods of 8, 16 and 24 hour stratified the lots in two levels. In results obtained by the principal component analysis it was verified. The variables traditional accelerated aging for 24 and 32 hours correlated with emergence in the field. Therefore, the traditional accelerated aging test at 42 °C for 24 hours are promising for evaluating the physiological quality of safflower seeds.

Germination and Vigor of Soybean and Corn Seeds Treated with Mixed Mineral Fertilizers

“…The principal component analysis of the soybean seed treatments was adequate, as the values of the principal components were similar to those found in studies evaluating soybean seed lots [ 28 ] and safflower vigor tests [ 29 , 30 ]. According to [ 31 ], a relatively small number of components were extracted (PC1 and PC2), with the ability to explain the highest variability in the original data.…”

“…The multivariate data analysis methods allow a global study of these variables, evidencing the connections, similarities, or differences between them with the least possible loss of information [ 26 , 29 , 30 , 31 , 34 ].…”

Contribution to the Optimization of Methods for Vigor Seed Evaluation of Camelina sativa (L.) Crantz

“…As evidenced in this investigation, a decrease in germination rates following the AA test was observed across numerous plant species: wheat [18], rise [19], oilseed rape [20], soybean [21], and safflower [22]. Elevated temperatures and high relative humidity levels to which seeds are subjected during the AA test and CD test induce heightened seed moisture content and increase the activity of hydrolytic enzymes.…”

Methodology of the accelerated aging test in tiny tomato seeds (solanum lycopersicum var. cerasiforme)

“…Barbosa et al (2011), when evaluating lettuce seeds by the accelerated aging method with saturated saline solution, found that the best exposure time to assess the potential of these seeds was 72 hours. Lopes et al (2010), also observed that the best period of exposure of okra seeds, using accelerated aging with saturated saline solution was 72 hours.…”

“…It was found that the water content of the seeds exposed to the saline solution (Table 5) showed lower values, after the aging periods in relation to those observed for the traditionally aged seeds. This indicates that the use of saline solution contributes to delay the absorption of water by the seeds in the accelerated aging test (Lopes et al 2010). Tunes et al (2012b) observed that in traditionally aged broccoli seeds, they reached higher water contents and with greater variations, when compared to aging with saline solution.…”