Substrate Supply for Effective Biocatalysis

Kim, Pei-Yi; Pollard, David; Woodley, John M.

doi:10.1021/bp060314b

Cited by 96 publications

(60 citation statements)

References 87 publications

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“…A list of some of the compounds used for transamination reactions is shown in Table VIII. From the table it is evident that for compounds such as acetophenone and homophenylalanine, a feeding strategy has to be employed to supply the substrate at a high concentration (Kim et al, 2007a). When a biocatalytic route is limited by substrate availability, whether due to low aqueous solubility, slow dissolution rate, or inhibition/toxicity, the controlled addition (feeding) of the substrate into the reaction medium is a common solution (D'Anjou and Daugulis, 2001;Doig et al, 2002;Lynch et al, 1997).…”

Section: Solubility Limitations and Use Of Solventsmentioning

confidence: 99%

Process considerations for the asymmetric synthesis of chiral amines using transaminases

Tufvesson

Lima-Ramos

Jensen

et al. 2011

Biotech & Bioengineering

222

183

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Biocatalytic transamination is being established as key tool for the production of chiral amine pharmaceuticals and precursors due to its excellent enantioselectivity as well as green credentials. Recent examples demonstrate the potential for developing economically competitive processes using a combination of modern biotechnological tools for improving the biocatalyst alongside using process engineering and integrated separation techniques for improving productivities. However, many challenges remain in order for the technology to be more widely applicable, such as technologies for obtaining high yields and productivities when the equilibrium of the desired reaction is unfavorable. This review summarizes both the process challenges and the strategies used to overcome them, and endeavors to describe these and explain their applicability based on physiochemical principles. This article also points to the interaction between the solutions and the need for a process development strategy based on fundamental principles.

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Section: Solubility Limitations and Use Of Solventsmentioning

confidence: 99%

Process considerations for the asymmetric synthesis of chiral amines using transaminases

Tufvesson

Lima-Ramos

Jensen

et al. 2011

Biotech & Bioengineering

222

183

View full text Add to dashboard Cite

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“…These include the application of various water-miscible organic solvents (Simon et al, 1998), water-immiscible organic solvents (Cruz et al, 2001), silicone oil (Kutney et al, 2000;Stefanov et al, 2006), polypropylene glycol (Kutney et al, 2000;Stefanov et al, 2006), cloud point system (Wang et al, 2004;, and microemulsions (Stefan et al, 2002). However, organic solvents present many drawbacks such as toxicity to microbial cells and environmental hazards (Schimid et al, 1998;Kim et al, 2007). Thus, the main focus of steroid biotransformation is to increase the solubility of hydrophobic substrates as well as retain the activity and stability of microbial cells.…”

Section: Introductionmentioning

confidence: 99%

Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08

Zhang

Peng

et al. 2013

J. Zhejiang Univ. Sci. B

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Biotransformation of phytosterol (PS) by a newly isolated mutant Mycobacterium neoaurum ZJUVN-08 to produce androstenedione has been investigated in this paper. The parameters of the biotransformation process were optimized using fractional factorial design and response surface methodology. Androstenedione was the sole product in the fermentation broth catalyzed by the mutant M. neoaurum ZJUVN-08 strain. Results showed that molar ratio of hydroxypropyl-β-cyclodextrin (HP-β-CD) to PS and substrate concentrations were the two most significant factors affecting androstenedione production. By analyzing the statistical model of three-dimensional surface plot, the optimal process conditions were observed at 0.1 g/L inducer, pH 7.0, molar ratio of HP-β-CD to PS 1.92:1, 8.98 g/L PS, and at 120 h of incubation time. Under these conditions, the maximum androstenedione yield was 5.96 g/L and nearly the same with the non-optimized (5.99 g/L), while the maximum PS conversion rate was 94.69% which increased by 10.66% compared with the non-optimized (84.03%). The predicted optimum conditions from the mathematical model were in agreement with the verification experimental results. It is considered that response surface methodology was a powerful and efficient method to optimize the parameters of PS biotransformation process.

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“…The final ester conversion (95.7%) was 1.04 times higher and obtained 2 h earlier than that of using the previous fed-batch system [6]. Moreover, this reaction system also produced a higher final ester conversion than any other esterification reactions using any other fed-batch systems [24,25,26,27].…”

Section: Bulletin Of Chemical Reaction Engineering and Catalysismentioning

confidence: 71%

Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution

Prastowo

Hidayat

Hastuti

2015

Bull. Chem. React. Eng. Catal.

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A fed-batch system was modified for the enzymatic production of Oleic Acid Ethyl Ester (OAEE) using rice bran (Oryza sativa) lipase by retaining the substrate molar ratio (ethanol/oleic acid) at 2.05:1 during the reaction. It resulted in an increase in the ester conversion of up to 76.8% in the first 6 h of the reaction, which was then followed by a decrease from 76.8% to 22.9% in 6 h later. The production of water in the reaction system also showed a similar trend. The water was hypothesized to lead lipase to reverse the reaction which resulted in a decrease in both (water and esters) in the last 6 h of the reaction. In order to overcome the problem, zeolite powder (25 and 50 mg/mL) were added into the reaction system at 5 h of the reaction. As the result, the final ester conversions increased drastically up to 90 -95.7%. Thus, the combination of a constant substrate molar ratio (ethanol/oleic acid) during the reaction (at 2.05:1) with the addition of zeolite powder (25 and 50 mg/mL) to the reaction system at 5 h is effective for the enzymatic synthesis of OAEE.

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Substrate Supply for Effective Biocatalysis

Cited by 96 publications

References 87 publications

Process considerations for the asymmetric synthesis of chiral amines using transaminases

Process considerations for the asymmetric synthesis of chiral amines using transaminases

Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08

Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution

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