Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution

Abstract

A fed-batch system was modified for the enzymatic production of Oleic Acid Ethyl Ester (OAEE) using rice bran (Oryza sativa) lipase by retaining the substrate molar ratio (ethanol/oleic acid) at 2.05:1 during the reaction. It resulted in an increase in the ester conversion of up to 76.8% in the first 6 h of the reaction, which was then followed by a decrease from 76.8% to 22.9% in 6 h later. The production of water in the reaction system also showed a similar trend. The water was hypothesized to lead lipase to reverse the reaction which resulted in a decrease in both (water and esters) in the last 6 h of the reaction. In order to overcome the problem, zeolite powder (25 and 50 mg/mL) were added into the reaction system at 5 h of the reaction. As the result, the final ester conversions increased drastically up to 90 -95.7%. Thus, the combination of a constant substrate molar ratio (ethanol/oleic acid) during the reaction (at 2.05:1) with the addition of zeolite powder (25 and 50 mg/mL) to the reaction system at 5 h is effective for the enzymatic synthesis of OAEE.

A Performance Study of Home-Made Co-Immobilized Lipase from Mucor miehei in Polyurethane Foam on The Hydrolysis of Coconut Oil to Fatty Acid

“…The production of fatty acids through hydrolysis of natural oils and fats is important to increase the economic value of renewable raw materials. Fatty acids are widely used in the food, cause it is not only able to increase energy efficiency but also eliminate by-product reactions compared to steam splitting process [2,[4][5][6].…”

Preface, BCREC Vol. 10 No. 3 Year 2015

“…Production of oleic acid ethyl ester catalyzed by crude rice bran (Oryza sativa) lipase in a modified fed-batch system was studied by Prastowo et al [2]. They focused on the combination (the modified fed-batch system) of a constant substrate molar ratio (ethanol/oleic acid) during the reaction and the addition of zeolite powder into the reaction system which resulted in higher final ester conversions (90-95.7%).…”