Substrate recognition by the Cdh1 destruction box receptor is a general requirement for APC/CCdh1-mediated proteolysis.

Abstract

There were several errors in this article. In the Cdc5 experiment in Fig. 3A, the anti-G6PD immunoblot image from the Spo13 experiment in Fig. 3B was accidentally duplicated and flipped horizontally. Also in Fig. 3A, the experiment labeled Nrm1 actually shows the results from the Mps1 experiment. In Fig. 4C, the Spo12 stability profile in the wild-type CDH1 strain is identical to that shown in Fig. 3B. In Fig. 4, B and C, a black bar is now used to indicate excision of lanes from the same exposure of the same immunoblot. A white vertical bar is still used to indicate comparison of different exposures where applicable. In Fig. 5A, the original Cdc5 panel showed a G6PD immunoblot image derived from the Kip1 panel shown in Fig. 4C. Although the images were not identical, an examination of the original data revealed that the actual Kip1 membrane was mistakenly used again to generate the G6PD load control image shown for the Cdc5 experiment. As a result, no appropriate load control data exists for the Cdc5 assay in the ABBA motif receptor mutant strain, and this panel has been removed from Fig. 5A. Additionally, in Fig. 5, only the positive results shown in Fig. 5B had three independent experiments. The negative results depicted in Fig. 5A were obtained in either one or two independent experiments. These errors have now been corrected and do not affect the results or conclusions of this work.

Phosphoproteome dynamics during mitotic exit in budding yeast

“…At anaphase onset, the anaphase‐promoting complex/cyclosome (APC/C) is activated to catalyze securin degradation and trigger chromosome segregation. At the same time, mitotic cyclins start to be degraded, followed by Polo and Aurora kinases (Shirayama et al , ; Lindon & Pines, ; Qin et al , ). Condensed mitotic chromosomes must first be fully separated on an elongating anaphase spindle, before the spindle subsequently disassembles and chromosomes decondense.…”

Development of D-box peptides to inhibit the Anaphase Promoting Complex/Cyclosome

“…In the past 15 years, high-resolution X-ray and cryo-EM (electron microscopy) studies of the APC/C and its interactions with substrates and E2s has generated a detailed description of the structure-function relationships that drive ubiquitination and degradation (Barford 2020). The binding of Cdc20 or FZR1 to the core APC/C creates at least three degron-binding sites for the known APC/C degrons, namely the "Destruction-box" (D-box, consensus RxxLxxxxN) and KEN motifs, and the ABBA motif thought to be required for Cyclin A degradation only (Qin et al 2017). A cryo-EM study of the structure of APC/C-FZR1 in complex with its pseudo-substrate inhibitor Acm1 revealed simultaneous engagement of D-box, KEN, and ABBA motifs of Acm1 with their respective receptor sites on the interactions (He et al 2013).…”

Characterization of the regulation of APC/CFZR-1 in the germline of Caenorhabditis elegans

“…The determination of the molecular structure of the APC/C complex indicated that coactivators are involved in the KEN-box recognition (Chao et al, 2012), while they partner with the Apc10 subunit to interact with the D-box motif (Da Fonseca et al, 2011). Strikingly the conformation changes occurring in the APC/C complex upon recognition of the D-boxes is a requirement to process all of the substrates, although many of them lack canonical D-boxes (Qin et al, 2017). Although it is not general, in some cases, APC/C substrates are regulated by phosphorylation of recognition motifs, resulting in the absence of APC/C recognition.…”