Substrate properties influencing ultrastructural differentiation of mammary epithelial cells in culture

Emerman, Joanne T.; Burwen, Susan Jo; Pitelka, Dorothy R.

doi:10.1016/0040-8166(79)90011-9

Cited by 175 publications

(78 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cell-ECM interaction, cell-cell interaction, and soluble factor stimulation are considered to be three major extrinsic factors essential for cystogenesis (Grobstein, 1956;Saxen and Sariola, 1987;Laurie et al, 1989;Wang et al, 1990a, b). Among these extrinsic factors, the ECM plays a key role in the differentiation and morphogenesis of epithelia (Emerman and Pitelka, 1977;Emerman et al, 1979;Parry et al, 1987;Lin and Bissell, 1993). For example, attachment of epithelial cells to the ECM is required for the establishment of cellular polarity and subsequent morphogenesis (Nelson, 1992;Rodriguez-Boulan and Powell, 1992).…”

Section: Introductionmentioning

confidence: 99%

The microenvironmental determinants for kidney epithelial cyst morphogenesis

Guo

Xia

Moshiach

et al. 2008

European Journal of Cell Biology

View full text Add to dashboard Cite

Although epithelial morphogenesis is tightly controlled by intrinsic genetic programs, the microenvironment in which epithelial cells proliferate and differentiate also contributes to the morphogenetic process. The roles of the physical microenvironment in epithelial morphogenesis, however, have not been well dissected. In this study, we assessed the impact of the microenvironment on epithelial cyst formation, which often marks the beginning or end step of morphogenesis of epithelial tissues and the pathological characteristic of some diseases. Previous studies have demonstrated that Madin-Darby canine kidney (MDCK) epithelial cells form cysts when grown in a three-dimensional (3D) extracellullar matrix (ECM) environment. We have now further demonstrated that the presence of ECM in the 3D scaffold is required for the formation of properly polarized cysts. Also, we have found that the full interface of epithelial cells with the ECM environment (in-3D) is not essential for cyst formation, since partial contact (on-3D) is sufficient to induce cystogenesis. In addition, we have defined the minimal ECM environment or the physical threshold for cystogenesis under the on-3D condition. Only above the threshold can the morphological cues from the ECM environment induce cyst formation. Moreover, cyst formation under the on-3D condition described in this study actually defines a novel and more feasible model to analyze in vitro morphogenesis. Finally, we have found that, during cystogenesis, MDCK cells generate basal microprotrusions and produce vesicle-like structures to the basal extracellular space, which are specific to and correlated with cyst formation. For the first

show abstract

Section: Introductionmentioning

confidence: 99%

The microenvironmental determinants for kidney epithelial cyst morphogenesis

Guo

Xia

Moshiach

et al. 2008

European Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…In vitro it has been shown that cells when cultured on materials are able to sense mechanical characteristics of the material and respond with a function in dependence on the mechanics [27]. Differentiation of mammary epithelial cells increased on soft collagen gel, as opposed to rigid tissue culture plastics [28], experiments with endothelial cells demonstrated a lower branching of the cellular network on stiffer materials compared with soft substrate [29] and similarly, neurons are branching more on soft material than on a stiff substrate [30]. A role of the stiffness of a material for regenerative processes was convincingly demonstrated when mesenchymal stem cells were cultured on hydrogels with varying stiffnesses [31].…”

Section: Stiffnessmentioning

confidence: 99%

Cell-material interaction

Rychly

Nebe

2013

BioNanoMaterials

View full text Add to dashboard Cite

Abstract:The interaction of cells with material surfaces is of fundamental relevance and contributes to the clinical success of implants. Cells sense their physico-chemical environment resulting in focal adhesion reorganization, spatio-temporal localization and activation of integrin dependent signalling proteins as well as phenotypic changes, e.g., of the actin cytoskeleton. The technology of designing instructive biomaterials involves chemical modifications by grafting of chemical groups, adhesion ligands and growth factors. Physical modifications of the materials are created by structuring the surfaces stochastically and geometrically and by modifications of the material stiffness. Insights into the mechanism at the interface that are involved in the regulation of cells such as stem cells, osteoblasts and chondrocytes by materials will advance the development of innovative biomaterials in regenerative medicine.

show abstract

“…Manipulating cellular and nuclear morphology in culture can induce a variety of functional changes, including glucose uptake and metabolism [2], proliferation [3][4][5], apoptosis [4], differentiation and gene expression [6][7][8][9][10]. These morphologically driven processes are controlled by the local microenvironment as exemplified in the mammary gland and mammary epithelial cells (for review see [11]).…”

Section: Introductionmentioning

confidence: 99%

Cell shape regulates global histone acetylation in human mammary epithelial cells

Beyec

Lee

et al. 2007

Experimental Cell Research

147

120

View full text Add to dashboard Cite

Extracellular matrix (ECM) regulates cell morphology and gene expression in vivo; these relationships are maintained in three-dimensional (3D) cultures of mammary epithelial cells. In the presence of laminin-rich ECM (lrECM), mammary epithelial cells round up and undergo global histone deacetylation, a process critical for their functional differentiation. However, it remains unclear whether lrECM-dependent cell rounding and global histone deacetylation are indeed part of a common physical-biochemical pathway. Using 3D cultures as well as nonadhesive and micropatterned substrata, here we showed that the cell 'rounding' caused by lrECM was sufficient to induce deacetylation of histones H3 and H4 in the absence of biochemical cues. Microarray and confocal analysis demonstrated that this deacetylation in 3D culture is associated with a global increase in chromatin condensation and a reduction in gene expression. Whereas cells cultured on plastic substrata formed prominent stress fibers, cells grown in 3D lrECM or on micropatterns lacked these structures. Disruption of the actin cytoskeleton with cytochalasin D phenocopied the lrECMinduced cell rounding and histone deacetylation. These results reveal a novel link between ECMcontrolled cell shape and chromatin structure, and suggest that this link is mediated by changes in the actin cytoskeleton.

show abstract

Substrate properties influencing ultrastructural differentiation of mammary epithelial cells in culture

Cited by 175 publications

References 13 publications

The microenvironmental determinants for kidney epithelial cyst morphogenesis

The microenvironmental determinants for kidney epithelial cyst morphogenesis

Cell-material interaction

Cell shape regulates global histone acetylation in human mammary epithelial cells

Contact Info

Product

Resources

About