Substrate Monolayers as Electrochemical Sensing Elements for α-Chymotrypsin

Resistance of zwitterionic telomers accumulated on metal surfaces against nonspecific adsorption of proteins

“…Previously, by the comparison with the data obtained by quartz crystal microbalance (QCM) method, it was reported that both the initial rate of increase in potential difference (d E p /dt) and the initial rate of decrease in peak current (d I/dt) are linearly correlated to the initial rate of protein adsorption to the SAM on the gold electrode [97]. be ascribed to an electrostatic attraction between the anionic protein (pI of BSA, 4.7-4.9 [3]) and the cationic SAM.…”

Kinetic study on the binding of lectin to mannose residues in a polymer brush

“…[20][21][22][23][24][25]. For the detection of enzymes, a SAM of a specific substrate or inhibitor of the enzymes was also constructed on a gold electrode [26,27]. * Moreover, -mercapto-polymer chain with many pendent glucose residues were accumulated on silver colloids as a "polymer brush" [28].…”

Accumulation of phenyl boronic acid-carrying telomers on a gold surface

“…Using both quartz crystal microbalance (QCM) and cyclic voltammetry methods, it was previously reported that the initial rate of increase in the E p value has a good correlation with the initial binding rate of enzyme molecules to the surfaceconfined substrate [51]. Fig.…”

“…In addition, SAMs of cyclodextrin derivatives were constructed on a gold electrode as a sensing device taking account of the ability of cyclodextrin in molecular recognition [45][46][47][48][49][50]. A SAM of specific substrate for α-chymotrypsin was also constructed on a gold electrode for the detection of the enzyme [51].…”