Substitutions of strontium in bioactive calcium silicate bone cements stimulate osteogenic differentiation in human mesenchymal stem cells

Huang, Tsui‐Hsien; Kao, Chia‐Tze; Shen, Yu-Fang; Lin, Yi-Ting; Liu, Yen-Ting; Yen, Ssu-Yin; Ho, Cheng-Yuan

doi:10.1007/s10856-019-6274-2

Cited by 32 publications

(32 citation statements)

References 43 publications

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“…Figure 2 showed the X-ray diffractometry analysis results for both the FGSr and FGSrB scaffolds. FGSrB had multiple peaks at 29.7° (C3S), 33.3° (Sr2SiO4), 35° (Ca(OH)2), and 32.8°, 35.2° and 40° (SrSiO3) corresponded with (104), (200), (101) crystallographic planes, respectively [29], which strongly demonstrated the presence of FGSr in the compound structure; this result was consistent with that of our previous study [17]. This indicated that the addition of BMP-2 did not interrupt the original structural characteristics of FGSr, thus allowing us to retain the original beneficial properties of FGSr.…”

Section: Scaffold Fabricationsupporting

confidence: 92%

“…In conclusion, the addition of BMP-2 was hypothesized to provide better osteoinduction and induce osteoblast development without affecting the mechanical properties of the scaffolds, thus increasing the suitability of FGSrB scaffolds in clinical use. [29], which strongly demonstrated the presence of FGSr in the compound structure; this result was consistent with that of our previous study [17]. This indicated that the addition of BMP-2 did not interrupt the original structural characteristics of FGSr, thus allowing us to retain the original beneficial properties of FGSr.…”

Section: Scaffold Fabricationsupporting

confidence: 91%

“…Such parameters were suggested to be superior characteristics for biomolecule loading and releasing [15]. Amongst the different types of ions such as zinc, magnesium and silicon, strontium (Sr) has received a considerable amount of attention in the field of BTE, as it was reported that Sr was able to enhance bone tissue regeneration [16,17]. Of which, it has been proven that Sr was able to simultaneously increase bone formation and decrease resorption.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Bone Morphogenic Protein-2-Loaded Mesoporous Strontium Substitution Calcium Silicate/Recycled Fish Gelatin 3D Cell-Laden Scaffold for Bone Tissue Engineering

Wang

Liu

et al. 2020

Processes

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Bone has a complex hierarchical structure with the capability of self-regeneration. In the case of critical-sized defects, the regeneration capabilities of normal bones are severely impaired, thus causing non-union healing of bones. Therefore, bone tissue engineering has since emerged to solve problems relating to critical-sized bone defects. Amongst the many biomaterials available on the market, calcium silicate-based (CS) cements have garnered huge interest due to their versatility and good bioactivity. In the recent decade, scientists have attempted to modify or functionalize CS cement in order to enhance the bioactivity of CS. Reports have been made that the addition of mesoporous nanoparticles onto scaffolds could enhance the bone regenerative capabilities of scaffolds. For this study, the main objective was to reuse gelatin from fish wastes and use it to combine with bone morphogenetic protein (BMP)-2 and Sr-doped CS scaffolds to create a novel BMP-2-loaded, hydrogel-based mesoporous SrCS scaffold (FGSrB) and to evaluate for its composition and mechanical strength. From this study, it was shown that such a novel scaffold could be fabricated without affecting the structural properties of FGSr. In addition, it was proven that FGSrB could be used for drug delivery to allow stable localized drug release. Such modifications were found to enhance cellular proliferation, thus leading to enhanced secretion of alkaline phosphatase and calcium. The above results showed that such a modification could be used as a potential alternative for future bone tissue engineering research.

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Section: Scaffold Fabricationsupporting

confidence: 92%

Section: Scaffold Fabricationsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Effect of Bone Morphogenic Protein-2-Loaded Mesoporous Strontium Substitution Calcium Silicate/Recycled Fish Gelatin 3D Cell-Laden Scaffold for Bone Tissue Engineering

Wang

Liu

et al. 2020

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“…As seen in Figure 2D, FGSr had multiple peaks at 29.7° (C3S), 33.3° (Sr2SiO4), 35° (Ca(OH)2) and 32.8°, 35.2° and 40° (SrSiO3) which strongly indicates that SrCS was present in the hydrogel scaffold. Furthermore, this result also implied that the addition of SrCS did not adversely affect the original structural properties of FG, thus suggesting that several beneficial properties of FG that were desired were retained in the FGSr scaffolds [15]. The Dynamic mechanical analysis (DMA) is conducted to consider the reinforcing effect brought by the addition of Sr ceramic in FG gel.…”

Section: Scaffold Fabricationmentioning

confidence: 92%

“…There were several studies indicating that CS-based materials were able to release Si ions gradually immersion and Si ions were found to not only increase regeneration of surrounding cells and tissues, but also induce bone mineralization by activating osteoblasts [13,14]. In addition, several studies had attempted to incorporate different ions (such as strontium and copper) into CS-based materials in order to fabricate CS scaffolds with varied and diverse functions [15,16], of which strontium (Sr) is a promising ion constantly shown to have beneficial effects for bone regeneration [17]. Furthermore, Sr-contained CS cements fabricated via the sol-gel process was found to not only enhance mechanical properties but also promote cell behaviors [18].…”

mentioning

confidence: 99%

Enhanced Proliferation and Differentiation of Human Mesenchymal Stem Cell-laden Recycled Fish Gelatin/Strontium Substitution Calcium Silicate 3D Scaffolds

Wang

Liu

et al. 2020

Applied Sciences

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Cell-encapsulated bioscaffold is a promising and novel method to allow fabrication of live functional organs for tissue engineering and regenerative medicine. However, traditional fabrication methods of 3D scaffolds and cell-laden hydrogels still face many difficulties and challenges. This study uses a newer 3D fabrication technique and the concept of recycling of an unutilized resource to fabricate a novel scaffold for bone tissue engineering. In this study, fish-extracted gelatin was incorporated with bioactive ceramic for bone tissue engineering, and with this we successfully fabricated a novel fish gelatin methacrylate (FG) polymer hydrogel mixed with strontium-doped calcium silicate powder (FGSr) 3D scaffold via photo-crosslinking. Our results indicated that the tensile strength of FGSr was almost 2.5-fold higher as compared to FG thus making it a better candidate for future clinical applications. The in-vitro assays illustrated that the FGSr scaffolds showed good biocompatibility with human Wharton jelly-derived mesenchymal stem cells (WJMSC), as well as enhancing the osteogenesis differentiation of WJMSC. The WJMSC-laden FGSr 3D scaffolds expressed a higher degree of alkaline phosphatase activity than those on cell-laden FG 3D scaffolds and this result was further proven with the subsequent calcium deposition results. Therefore, these results showed that 3D-printed cell-laden FGSr scaffolds had enhanced mechanical property and osteogenic-related behavior that made for a more suitable candidate for future clinical applications. Furthermore, bone regeneration is a complex physiological process regulated by osteoclast resorption with osteoblast bone formation and the entire process includes inflammatory reactions, endochondral bone formation and bone remodeling. Currently, for clinical cases, the golden method to repair bone defects is to use autologous bone grafts as this method avoids disease spreading and immunological rejection. However, autografts have some major drawbacks such as limited availability and invasive harvesting that requires additional surgery to harvest healthy bones from another site. Furthermore, additional surgeries mean increased surgical risks and complications [2]. Allograft is an alternative to bone repair; however, it is highly limited due to rejection and lack of availability. To solve this problem, researchers had attempted to develop novel biomaterials including ceramics, polymers and composites as an alternative choice for bone regeneration [3][4][5]. In order to promote bone healing, the material must be biocompatible, biodegradable and able to promote bone regeneration which mimics the distinctive property of natural bone [6]. With the emergence of newer 3D printing techniques, we are now able to fabricate scaffolds with customized shape, pore sizes and architectures that are similar to native bone [7,8].For many years, bioceramics has emerged as promising biomaterial for bone regeneration [9,10]. Calcium phosphate is one of the most popular bioceramics which had been repeate...

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Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium‐doped hydroxyapatite as promising biomaterials

Grazioli

Silva

Souza

et al. 2020

J Biomedical Materials Res

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Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr‐doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications. HA and HASr were synthesized by a wet‐precipitation method and added to the composite hydrogels in fractions up to 15 wt%. Physical–chemical characterizations of particles and hydrogels included scanning electron microscopy, energy‐dispersive spectroscopy, Fourier‐transform infrared spectroscopy, X‐ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability. Particles were irregular in shape and appeared to have narrow size variation. The thermal behavior of composite hydrogels was altered compared to the control (bare) hydrogel. All hydrogels exhibited high porosity. HA/HASr particles reduced total porosity without reducing pore size. The mechanical strength was improved as the fraction of HA or HASr was increased. HASr particles led to a faster water uptake but did not interfere with the total hydrogel swelling capacity. In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect. In conclusion, PVA/CS hydrogels containing HA or HASr particles were successfully synthesized and showed promising morphological, mechanical, and swelling properties, which are particularly required for scaffolding.

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Substitutions of strontium in bioactive calcium silicate bone cements stimulate osteogenic differentiation in human mesenchymal stem cells

Cited by 32 publications

References 43 publications

Effect of Bone Morphogenic Protein-2-Loaded Mesoporous Strontium Substitution Calcium Silicate/Recycled Fish Gelatin 3D Cell-Laden Scaffold for Bone Tissue Engineering

Effect of Bone Morphogenic Protein-2-Loaded Mesoporous Strontium Substitution Calcium Silicate/Recycled Fish Gelatin 3D Cell-Laden Scaffold for Bone Tissue Engineering

Enhanced Proliferation and Differentiation of Human Mesenchymal Stem Cell-laden Recycled Fish Gelatin/Strontium Substitution Calcium Silicate 3D Scaffolds

Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium‐doped hydroxyapatite as promising biomaterials

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