Substitutional impact on biological activity of new water soluble Ni(II) complexes: Preparation, spectral characterization, X-ray crystallography, DNA/protein binding, antibacterial activity and in vitro cytotoxicity

Umadevi, C.; Kalaivani, P.; Puschmann, Horst; Sevanan, Murugan; Mohan, P. S.; Prabhakaran, R.

doi:10.1016/j.jphotobiol.2016.12.015

Cited by 17 publications

(8 citation statements)

References 54 publications

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“…The effect of 1 and 3 on BSA synchronous fluorescence spectroscopy is shown in Figure S13 in the Supporting Information. From these results it is concluded that the test compounds affect the tryptophan microenvironment significantly but do not affect the microenvironment of tyrosine residues during the binding process …”

Section: Resultsmentioning

confidence: 87%

Half-Sandwich Iridium and Ruthenium Complexes: Effective Tracking in Cells and Anticancer Studies

Guo

Tian

et al. 2018

Inorg. Chem.

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Half-sandwich metal-based anticancer complexes suffer from uncertain targets and mechanisms of action. Herein we report the observation of the images of half-sandwich iridium and ruthenium complexes in cells detected by confocal microscopy. The confocal microscopy images showed that the cyclopentadienyl iridium complex 1 mainly accumulated in nuclei in A549 lung cancer cells, whereas the arene ruthenium complex 3 is located in mitochondria and lysosomes, mostly in mitochondria, although both complexes entered A549 cells mainly through energy-dependent active transport. The nuclear morphological changes caused by Ir complex 1 were also detected by confocal microscopy. Ir complex 1 is more potent than cisplatin toward A549 and HeLa cells. DNA binding studies involved interaction with the nucleobases 9-ethylguanine, 9-methyladenine, ctDNA, and plasmid DNA. The determination of bovine serum albumin binding was also performed. Hydrolysis, stability, nucleobase binding, and catalytic NAD + / NADH hydride transfer tests for complexes 1 and 3 were also carried out. Both complexes activated depolarization of mitochondrial membrane potential and intracellular ROS overproduction and induced cell apoptosis. Complex 3 arrested the cell cycle at the G 0 /G 1 phase by inactivation of CDK 4/cyclin D1. This work paves the way to track and monitor half-sandwich metal complexes in cells, shines a light on understanding their mechanism of action, and indicates their potential application as theranostic agents.

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Section: Resultsmentioning

confidence: 87%

Half-Sandwich Iridium and Ruthenium Complexes: Effective Tracking in Cells and Anticancer Studies

Guo

Tian

et al. 2018

Inorg. Chem.

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“…If the quenching mechanism is a static quenching mechanism, changes in the absorption intensity (278 nm) are accompanied by significant wavelength shifts, whereas dynamic quenching takes place without any shifts in wavelength . In Figure a, we can see that there is no shift in wavelength at 278 nm after the addition of complexes 1 – 6 to a solution of BSA, which indicates that the interaction between BSA and complexes takes the form of dynamic quenching …”

Section: Resultsmentioning

confidence: 94%

“…[12] In Figure 3a, we can see that there is no shifti nw avelength at 278 nm after the addition of com- plexes 1-6 to as olution of BSA, whichi ndicates that the interaction between BSA and complexes takes the form of dynamic quenching. [30] Fluorescenceq uenching is another effective method to study the interaction between complexes and proteins. As shown in Figure3b( see the Supporting Information), as the amount of complex 3 increases, the fluorescencei ntensity of BSA (10 mm)a t3 43 nm decreases continuously.…”

Section: Study Of Bsai Nteractionsmentioning

confidence: 99%

Triphenylamine‐Appended Half‐Sandwich Iridium(III) Complexes and Their Biological Applications

Tian

Liu

et al. 2018

Chemistry An Asian Journal

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Organometallic half-sandwich Ir complexes of the type [(η -Cp )Ir(N^N)Cl]PF (Cp : Cp* or its phenyl Cp or biphenyl Cp derivatives; N^N: triphenylamine (TPA)-substituted bipyridyl ligand groups) were synthesized and characterized. The complexes showed excellent bovine serum albumin (BSA) and DNA binding properties and were able to oxidize NADH to NAD (NAD=nicotinamide adenine dinucleotide) efficiently. The complexes induced apoptosis effectively and led to the emergence of reactive oxygen species (ROS) in cells. All complexes showed potent cytotoxicity with IC values ranging from 1.5 to 7.1 μm toward A549 human lung cancer cells after 24 hours of drug exposure, which is up to 14 times more potent than cisplatin under the same conditions.

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“…Investigations on interactions of DNA and thiosemicarbazone molecules have attracted significant attention over the last years (10). The square planar Ni(II)thiosemicarbazone complexes were reported to have DNA interaction and topoisomerase II inhibition activity (11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

“…Binding affinity in CT-DNA and protein and cytotoxicity activity against cancer cell lines HeLa, A549 and HepG2 of Ni(II) complexes consisting of 4-methoxysalicylaldehyde-N 4 -Rthiosemicarbazone (R: H, Me, Et, Ph) and PPh3 were investigated by Prabhakaran et al The results showed that the complexes have important binding ability and cytotoxicity activity in contrast of their ligands. The binding affinity towards DNA and protein is decreased in order of C2H5>CH3>H>C6H5 unlike in order of cytotoxic activity (11). Another paper of Prabhakaran is on DNA topoisomerase II inhibition activity of nickel(II) complexes consisted of salicylaldehyde-N 4 -R (R: Me, Ph)/2-hydroxynaphthaldehyde-N 4 -R (R: Me)-thiosemicarbazone and PPh3.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of DNase I Enzyme with Nickel(II) Triphenylphosphine Complexes Incorporating Tridentate Schiff Base Ligands in Vitro

Güveli

2018

Journal of the Turkish Chemical Society Section A: Chemistry

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The nickel(II) complexes containing with ONS chelating 3-methoxy-salicylaldehyde-N 4-R thiosemicarbazones (R:-H2,-propyl) and triphenylphosphine coligands have been synthesized. The structures of Ni(II)-centered metal complexes were approved by means of analytical and spectroscopic data. The solid-state structure of complex 2 bearing PPh3 as co-ligand was clarified by single crystal X-ray crystallography, which revealed square planar geometry around Ni(II) ion. The potential of these complexes to inhibit the DNase I enzyme, which uses DNA as a substrate, was investigated in vitro. The results revealed that the compounds inhibited the DNase enzyme directly and/or indirectly (by masking of DNA molecules) at ≥0.1 µg/mL concentrations in vitro.

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Substitutional impact on biological activity of new water soluble Ni(II) complexes: Preparation, spectral characterization, X-ray crystallography, DNA/protein binding, antibacterial activity and in vitro cytotoxicity

Cited by 17 publications

References 54 publications

Half-Sandwich Iridium and Ruthenium Complexes: Effective Tracking in Cells and Anticancer Studies

Half-Sandwich Iridium and Ruthenium Complexes: Effective Tracking in Cells and Anticancer Studies

Triphenylamine‐Appended Half‐Sandwich Iridium(III) Complexes and Their Biological Applications

Inhibition of DNase I Enzyme with Nickel(II) Triphenylphosphine Complexes Incorporating Tridentate Schiff Base Ligands in Vitro

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