Substantial discrepancies in 17β‐oestradiol concentrations obtained with three different commercial direct radioimmunoassay kits in rat sera

Effects of stress early in gestation on hippocampal neurogenesis and glucocorticoid receptor density in pregnant rats

“…Blood samples were collected from intracardiac puncture, stored at 4°C overnight and centrifuged at 10 Â g for 15 min. Samples were run in duplicate using a commercially available RIA kit for estradiol (DPC 125I radioimmunoassay kit, Siemens Healthcare Diagnostics Inc., Washington, D.C., USA) based on (Strom et al, 2008). The average intra-assay coefficient of variation was below 10%.…”

Pregnancy or stress decrease complexity of CA3 pyramidal neurons in the hippocampus of adult female rats

“…Serum levels of corticosterone and estradiol were assessed as previously described on a subset of females (n = 5-7/group) (Strom et al, 2008;Pawluski et al, 2009bPawluski et al, , 2012a. To do this, trunk blood was taken at sacrifice, stored at 4°C overnight and centrifuged at 10g for 10 min.…”

“…The assay had a sensitivity of 7.7 ng/mL. For serum estradiol levels, a commercially available RIA kit for estradiol (DPC 125I radioimmunoassay kit, Siemens Healthcare Diagnostics Inc., Belgium) was used based on Strom et al (2008). The average intra-assay coefficients of variation were below 3%.…”

Quantification of 2-hydrazinopyridine derivatized steroid hormones in fathead minnow (Pimephales promelas) blood plasma using LC-ESI+/MS/MS

“…Despite their sensitivity, these methods are cited as being prone to antibody cross-reactivity with hormones sharing conserved structural groups, with further limitations including the relatively small numbers of hormones that can be quantified per sample and the hazard associated with the use of radioactive materials [22][23][24][25][26]. In contrast to these methods, liquid chromatography coupled with mass spectrometry (LC-MS) and/or tandem mass spectrometry (LC-MS/MS) has increased in use.…”