Pregnancy or stress decrease complexity of CA3 pyramidal neurons in the hippocampus of adult female rats

Pawluski, Jodi L.; Valença, Andreia; Santos, Ana Isabel; Costa-Nunes, João Pedro; Steinbusch, Harry W.M.; Strekalova, Tatyana

doi:10.1016/j.neuroscience.2012.09.059

Cited by 44 publications

(38 citation statements)

References 55 publications

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“…To assay serum corticosterone, trunk blood was stored at 4 C overnight and centrifuged at 10 Â g for 10 min; the assay was performed as previously described (Pawluski et al, 2012). Serum was collected and stored a À80 C until use.…”

Section: Corticosterone Concentrationmentioning

confidence: 99%

Altered emotionality, hippocampus-dependent performance and expression of NMDA receptor subunit mRNAs in chronically stressed mice

Costa-Nunes

Зубарева

Araújo-Correia

et al. 2013

Stress

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N-Methyl-D-aspartate receptor (NMDAR)-mediated neurotransmission in the hippocampus is implicated in cognitive and emotional disturbances during stress-related disorders. Here, using quantitative RT-PCR, we investigated the hippocampal expression of NR2A, NR2B and NR1 subunit mRNAs in a mouse stress paradigm that mimics clinically relevant conditions of simultaneously affected emotionality and hippocampus-dependent functions. A 2-week stress procedure, which comprised ethologically valid stressors, exposure to a rat and social defeat, was applied to male C57BL/6J mice. For predation stress, mice were introduced into transparent containers that were placed in a rat home cage during the night; social defeat was applied during the daytime using aggressive CD1 mice. This treatment impaired hippocampusdependent performance during contextual fear conditioning. A correlation between this behavior and food displacement performance was demonstrated, suggesting that burrowing behavior is affected by the stress procedure and is hippocampus-dependent. Stressed mice (n ¼ 22) showed behavioral invigoration and anomalous anxiolytic-like profiles in the O-maze and brightly illuminated open field, unaltered short-term memory in the step-down avoidance task and enhanced aggressive traits, as compared to non-stressed mice (n ¼ 10). Stressed mice showed increased basal serum corticosterone concentrations, hippocampal mRNA expression for the NR2A subunit of the NMDAR and in the NR2A/NR2B ratio; mRNA expression of NR2B and NR1 was unchanged. Thus, stress-induced aberrations in both hippocampal-dependent performance and emotional abnormalities are associated with alterations in hippocampal mRNA NR2A levels and the NR2A/NR2B ratio and not with mRNA expression of NR2B or NR1.

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Section: Corticosterone Concentrationmentioning

confidence: 99%

Altered emotionality, hippocampus-dependent performance and expression of NMDA receptor subunit mRNAs in chronically stressed mice

Costa-Nunes

Зубарева

Araújo-Correia

et al. 2013

Stress

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“…Recent work has shown that repeated stress during late pregnancy increases the number of proliferating cells in the hippocampus of the late pregnant female, but has no effect on new cell survival during gestation . Stress during the last 2 weeks of gestation also results in significantly shorter apical dendrites and fewer apical branch points in CA3 pyramidal cells in late pregnant females (Pawluski et al, 2012c). However, further work is needed to understand how stress early in gestation may affect hippocampal plasticity in the pregnant female.…”

Section: Introductionmentioning

confidence: 99%

Effects of stress early in gestation on hippocampal neurogenesis and glucocorticoid receptor density in pregnant rats

et al. 2015

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“…The authors concluded that neurons of the inferalimbic area but not the prelimbic area were sensitive to even minor stress. In another study, stress reduced the numbers of pyramidal cells in the CA3 hippocampal area of pregnant rats during pregnancy but had no effect on hippocampal CA1 cells (22). Immobilization stress caused atrophy in apical dendrites of Betz cells in the fifth layer of the prefrontal cortex in rats (23).…”

Section: Discussionmentioning

confidence: 93%

The Effects of Different Types of Chronic Stress on Morphometric Changes and Apoptosis of Betz Cells in the Internal Pyramidal Layer of the Cerebral Cortex of Rats

Sabernia¹,

Piri²,

Rajaei³

2016

Avicenna J Med Biochem

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Background: Technology-induced increases in stress can have adverse effects on the nervous system. Objectives: The aim of the present study was to investigate the effects of chronic multiple stressors on morphometric changes in Betz cells in the rat cerebral cortex. Methods: Eighteen Wistar rats were divided randomly into two equal groups. One group was then exposed to different types of stress (forced swimming, restraint, water deprivation, isolation, and food deprivation) for 10 days, and the other group (control) remained in cages and was not exposed to any stress. The animals were then weighed and anesthetized, and their brains were removed and weighed. After fixation, the brain samples were prepared for light microscopy study. The number, size, and incidence of apoptosis of Betz cells in the internal pyramidal layer were determined using the ImageTool software program, and the data were analyzed using SPSS software and a t-test, with a value of P < 0.05 considered statistically significant. Results:The mean number and size of Betz cells in the stressed group decreased significantly compared to those in the control group (P < 0.05). Qualitative observations revealed chromatolysis of Nissl bodies, nucleus condensation, and a decrease in neural processes. The mean number of apoptotic cells in the stressed group were also significantly increased compared to that of the control group (P < 0.001). Conclusions:The results showed that chronic multiple sequential stress can have negative effects on the cerebral cortex of rats by reducing the size and number of Betz cells and increasing the incidence of apoptosis. More studies are needed to confirm these results.

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Pregnancy or stress decrease complexity of CA3 pyramidal neurons in the hippocampus of adult female rats

Cited by 44 publications

References 55 publications

Altered emotionality, hippocampus-dependent performance and expression of NMDA receptor subunit mRNAs in chronically stressed mice

Altered emotionality, hippocampus-dependent performance and expression of NMDA receptor subunit mRNAs in chronically stressed mice

Effects of stress early in gestation on hippocampal neurogenesis and glucocorticoid receptor density in pregnant rats

The Effects of Different Types of Chronic Stress on Morphometric Changes and Apoptosis of Betz Cells in the Internal Pyramidal Layer of the Cerebral Cortex of Rats

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