Substance P regulates macrophage inflammatory protein 3α/chemokine C-C ligand 20 (CCL20) with heme oxygenase-1 in human periodontal ligament cells

Abstract

SummaryAlthough substance P (SP), a potent proinflammatory peptide, is involved in inflammation and immune responses, the effect of SP on the expression of macrophage inflammatory protein 3a [MIP-3a, chemokine C-C ligand 20 (CCL20)] in periodontal ligament (PDL) cells is unknown. Equally enigmatic is the link between SP, the stress protein heme oxygenase-1 (HO-1), and CCL20 production. We investigated whether SP induces the release of chemokine CCL20 from immortalized PDL (

The role of heme oxygenase-1 in mechanical stress- and lipopolysaccharide-induced osteogenic differentiation in human periodontal ligament cells

“…[19][20][21][22][23][24] In the present study, we found that MS increased HO-1 mRNA and protein expression in PDLCs. These findings are consistent with our previous study in which osteogenic differentiation Figure 4.…”

“…21,22,24 Increases in the expression of HO-1, BMP-2, BMP-7, and Runx-2 resulting from treatment with LPS and MS were attenuated by preincubation with selective inhibitors of p38, ERK, PI3K, PKG, and NF-kB (SB203580, U0126, LY294002, KT5823, and PDTC, respectively). These results suggest that LPS-induced and MS-induced HO-1 and BMP expression occurs via the activation of p38, ERK MAPK, PI3K, PKG, and NF-kB.…”

“…Previously, we reported that the HO-1 pathway is a key mechanism for the adaptation to stressful conditions and the recovery from injurious events by dental cells. [19][20][21][22][23][24] Moreover, the expression of HO-1 is related to adipogenesis by human MSCs, 25 osteoblastic differentiation by PDLCs 23 and neuronal differentiation by MSCs. 26 Because HO-1 is associated with differentiation, it is plausible to postulate that HO-1 is involved in the response of PDLCs to MS and bacterial infection as well as in the differentiation of PDLCs to osteoblast-like cells.…”

Anti-inflammatory effects of apigenin on nicotine- and lipopolysaccharide-stimulated human periodontal ligament cells via heme oxygenase-1

“…Previously, we reported that the HO-1 pathway is a key mechanism for both the adaptation of cells to stressful conditions and their recovery from injurious events in human pulp and PDL cells [27][28][29][30][31][32]. Moreover, HO-1 has therapeutic potential in hepatoprotection [33], inflammatory arthritis [34], psoriasiform skin lesions [35], neuroinflammation [36], inflammatory bowel disease [37], and periodontitis [15].…”

The Role of Heme Oxygenase-1 in the Proliferation and Odontoblastic Differentiation of Human Dental Pulp Cells

“…Previously, we reported that the HO-1 pathway plays a key role in the adaptation of cells to stressful conditions and the recovery of HDPCs and periodontal ligament cells (PDLCs) from injurious events (7)(8)(9)(10)(11)(12). Moreover, several studies have shown that HO-1 expression is related to adipogenesis in human mesenchymal stem cells (MSCs) (13), osteoblastic differentiation in PDLCs (7,12), and neuronal differentiation in MSCs (14).…”