Anti-inflammatory effects of apigenin on nicotine- and lipopolysaccharide-stimulated human periodontal ligament cells via heme oxygenase-1

Jeong, Gil‐Saeng; Lee, Seoung‐Hoon; Jeong, Seong‐Nyum; Kim, Youn‐Chul; Kim, Eun-Cheol

doi:10.1016/j.intimp.2009.08.015

Cited by 73 publications

(61 citation statements)

References 51 publications

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“…Previous studies have shown that LPS and nicotine is a cytotoxic agent to human fibroblasts derived from periodontium 28,29) . In this study, we present evidence of LPS plus nicotine-induced cytotoxicity on hPDLCs by inhibiting cell growth and proliferation in a dose-dependent manner.…”

Section: Discussionmentioning

confidence: 98%

NFATc Mediates Lipopolysaccharide and Nicotine-Induced Expression of iNOS and COX-2 in Human Periodontal Ligament Cells

Lee¹,

Yu²

2015

Journal of dental hygiene science

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Although nuclear factor of activated T cell (NFAT) plays a key role in inflammation, its anti-inflammatory effects and mechanism of action in periodontitis are still unknown. This study aimed to identify the effects of NFAT on the proinflammatory mediators activated by lipopolysaccharide (LPS) plus nicotine stimulation in human periodontal ligament cells (hPDLCs). The production of nitric oxide (NO) and prostaglandin E2 (PGE2) was evaluated using Griess reagent and an enzyme immunoassay, respectively. The expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and NFAT proteins was evaluated by Western blot analysis. LPS plus nicotine synergistically induced the production of NO and PGE2 and increased the protein expression of iNOS, COX-2 and NFAT. Treatment with an NFAT inhibitor blocked the LPS plus nicotine-stimulated NO and PGE2 release as well as the expression of iNOS and COX-2. Our data suggest that the LPS plus nicotine-induced inflammatory effects on hPDLCs may act through a novel mechanism involving the action of NFAT. Thus, NFAT may provide a potential therapeutic target for the treatment of periodontal disease associated with smoking and dental plaque.

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Section: Discussionmentioning

confidence: 98%

NFATc Mediates Lipopolysaccharide and Nicotine-Induced Expression of iNOS and COX-2 in Human Periodontal Ligament Cells

Lee¹,

Yu²

2015

Journal of dental hygiene science

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“…The flavonoid apigenin, a constituent of MTC extract, has important anti-inflammatory and antioxidant activities. It inhibits nitric oxide (NO) production and the activities of hyaluronidase, collagenase, and the cyclooxygenases, enzymes that play key roles in the inflammatory process (6). The anti-inflammatory activity of apigenin was also observed in in vitro studies of human periodontal ligament cells stimulated with nicotine and lipopolysaccharide.…”

Section: Discussionmentioning

confidence: 99%

“…MTC also contains the flavonoids apigenin, quercetin, patuletin, and luteolin (5). The anti-inflammatory and antioxidant effects of apigenin and quercetin have been extensively described (6,7).…”

Section: Introductionmentioning

confidence: 99%

Clinical efficacy of a 1% <i>Matricaria chamomile </i>L. mouthwash and 0.12% chlorhexidine for gingivitis control in patients undergoing orthodontic treatment with fixed appliances

et al. 2016

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“…Apigenin has long been considered to have various biological activities such as anti-inflammatory [16] and antitumorigenic effect [17] in various cell types. The basic structure of cholesterol is a sterol nucleus.…”

Section: Resultsmentioning

confidence: 99%

In Silico Study of Apigenin and Apigetrin as Inhibitor of 3-Hydroxy-3-Methyl-Glutayl-Coenzyme a Reductase

Lestari

Sukandar

Fidrianny

2017

Asian J Pharm Clin Res

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Objective: The objectives of this research were to investigate in silico interaction between apigenin and apigetrin with 3-hydroxy-3-methyl-glutaylcoenzyme A (HMG Co-A) reductase, to find the most favorable binding site as well as to predict the binding mode. Materials and Methods:Docking calculation was performed by branded Sony Vaio PC Linux Ubuntu 14.04 LTS. The binding process based on the best docking result with HMG Co-A reductase was presented in two-dimensional diagram. Statin, atorvastatin, and R-mevalonate were used as standard.Results: Binding affinity and inhibition constant of R-mevalonate were Ei=−4.2 kcal/mol, Ki=836.78 µM; apigenin Ei=−7.0 kcal/mol, Ki=7.43 µM; apigetrin Ei=−5.9 kcal/mol, Ki=47.53 µM; simvastatin Ei=−8.2 kcal/mol; Ki=0.98 µM; atorvastatin Ei=−8.4 kcal/mol; Ki=0.7 µM. Apigenin had better binding interaction than apigetrin. Conclusion:Apigenin could be developed as anticholesterol.

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Anti-inflammatory effects of apigenin on nicotine- and lipopolysaccharide-stimulated human periodontal ligament cells via heme oxygenase-1

Cited by 73 publications

References 51 publications

NFATc Mediates Lipopolysaccharide and Nicotine-Induced Expression of iNOS and COX-2 in Human Periodontal Ligament Cells

NFATc Mediates Lipopolysaccharide and Nicotine-Induced Expression of iNOS and COX-2 in Human Periodontal Ligament Cells

Clinical efficacy of a 1% <i>Matricaria chamomile </i>L. mouthwash and 0.12% chlorhexidine for gingivitis control in patients undergoing orthodontic treatment with fixed appliances

In Silico Study of Apigenin and Apigetrin as Inhibitor of 3-Hydroxy-3-Methyl-Glutayl-Coenzyme a Reductase

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