Subsets of migrating intestinal dendritic cells

Abstract

SummaryDendritic cells in the intestine are heterogeneous. Phenotypically different populations of conventional dendritic cells have been identified in the intestinal lamina propria, Peyer's patches, and in the draining mesenteric lymph nodes, to which these DCs constitutively migrate. Markers used to identify these populations include MHC class II, CD11c, CD8α, CD11b, and CD103. Extensive studies in rats, summarised here, which involved collection of migrating DCs by thoracic duct cannulation after mesenteric lymphadenectomy, have clearly demonstrated that the subsets of migrating intestinal lymph DCs have different functional properties. The subsets might play different roles in the induction of oral tolerance and in driving systemic immune responses after vaccination or intestinal stimulation with TLR ligands. The use of these surgical techniques allows investigation of the functions of purified subsets of migrating DCs. However, in the rat these studies are limited by the range of available reagents, and are difficult to compare with data from other species in this fast-moving field. Recent refinements have enabled the collection of migrating intestinal DCs from mice; our initial results are described here. We believe that these studies will generate exciting data and have the potential to resolve important questions about the functions of migrating intestinal DC subsets.

Neuroenteric axis modulates the balance of regulatory T cells and T-helper 17 cells in the mesenteric lymph node following trauma/hemorrhagic shock

“…DCs in the intestinal mucosa play a crucial role in directing immune responses to luminal antigens (20,32). Intestinal DCs can efficiently migrate to MLNs, thereby providing a critical link to adaptive T cell immunity (51).…”

Differential Effects of Viral Vectors on Migratory Afferent Lymph Dendritic CellsIn VitroPredict Enhanced ImmunogenicityIn Vivo

“…The collection of migrating DC using this method has provided invaluable information on their phenotype and functions. It has been possible to cannulate the lymphatic vessels of a number of animal species; however, the procedure remains technically challenging and successful only for the thoracic duct in the rat and other lymph vessels in large animals (2,16,24,44). These lymph DC are a valuable reagent to study the interaction with potential vaccine antigens because they can be used directly ex vivo without extensive in vitro manipulations.…”

Ethanol affects the absorption and tissue distribution of orally administered antigens in mice

“…p (a/b) < 0.05 or p (*) < 0.05 represents a significant difference between groups. (Onodera et al 2009;Milling et al 2010). These results suggested that there was a relationship between the suppression of ethanol-mediated IgA responses and the decrease in palmitoyl-ova transport to MLNs.…”