Abstract. We have fabricated submicron YBa 2 Cu 3 O 7-x grain boundary junctions using [100] tilt bicrystal substrates. In these substrates, crystal planes are tilted around the b-axis, parallel to the bicrystal line. We have obtained high-quality junctions and fitted experimental data using a recently theory accounting for d-wave symmetry of the order parameter in layered superconductors with arbitrary orientation. In our experiment, sensitive to the amplitude of the pairing state, lobes of different signs do not influence the electrical transport in the junction. Nevertheless, the effects of a non-conventional symmetry are clearly observed on the temperature dependence of the critical current.Since their discovery, an impressive number of papers dedicated to the understanding of physical mechanisms in high-critical temperature superconductors has been produced. Among these materials, intensive studies have been pursued on YBa 2 Cu 3 O 7-x (YBCO) superconductor, that has generated large curiosity in the researchers, because of its complicated phenomenology. One of the methods largely used for analyzing properties of superconductive materials is the Josephson effect [1]. The study of the Josephson effect allows the analysis of several electrical and magnetic phenomena, mirroring intrinsic properties of the junction. Typically planar technologies, used for the fabrication of tunnel junctions with conventional superconductors, generally failed. Indeed, the coherence lengths extremely short in YBCO, of the order of 2 nm in the plane and even shorter along the c-axis, forced the scientific community to look for alternative techniques for the fabrication of Josephson devices. Since then, several technologies have been introduced, as for instance junctions known as step-edge [2], rampedge [3], or grain boundary junctions [4]. The simplest and most used method was the fabrication of grain boundary junctions (GBJs) through the employment of bicrystal [4], tricrystal [5], or tetracrystal [6] substrates. This was also the fastest method for fabricating YBCO junctions and starting the analysis of electrical properties of such fascinating superconductors. Thanks to such technologies, most of exotic properties have been determined. In particular, the most important difference with respect conventional superconductors is the d-wave symmetry of the order parameter, in the form d x2-y2 [7]. This important result has been determined through phase-sensitive experiments using peculiar properties of dc-SQUIDs [5,7,8] (Superconducting Quantum Interference Devices) or rf-SQUIDs [9]. About this point, more than one convincing experiment has been carried out. However, to date, whether the symmetry of the order parameter in YBCO is fully represented by a d x2-y2 symmetry [5,10] or small components of other symmetries [11] are present, is still matter of debate. A step forward