Submicromolar quantification of pyocyanin in complex biological fluids using pad-printed carbon electrodes

Burkitt, Richard; Sharp, Duncan

doi:10.1016/j.elecom.2017.03.021

Cited by 14 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pseudomonas aeruginosa is a Gram-negative bacteria and human pathogen that is responsible for a high number of hospital infections and is prevalent in patients with compromised immune systems, chronic wounds, and in the lungs of those suffering from cystic fibrosis. , Several redox-active phenazine derivatives 2-alkyl-4-quinolones (AHQs) have been identified that behave as QS molecules produced by P. aeruginosa, e.g., 4-hydroxy-2-heptylquinoline (HHQ) and 2-heptyl-3,4-dihydroxyquinoline (pseudomonas quinolone signal, PQS), see Figure for chemical structures. ,,,,− AHQs exist in another tautomeric form and can also be termed 4-hydroxy-2-alkylquinolines (HAQs) . The neutral 4-quinolone rather than the 4-hydroxy-quinoline is the predominant species in the pH range 4–6 .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection ofPseudomonas aeruginosaQuorum Sensing Molecules at a Liquid|Liquid Interface

et al. 2019

View full text Add to dashboard Cite

Opportunistic pathogenic bacteria, such as Pseudomonas aeruginosa, pose a serious risk to patients suffering from a compromised immune system and those patients with cystic fibrosis. Confirming their presence often requires culturing the bacteria, which can take days. Herein is proposed a rapid electrochemical detection method based on P. aeruginosa-generated small molecules employed for chemical communication, referred to as quorum sensing (QS) molecules, within the biofilm formed by the bacteria; specifically, 4-hydroxy-2-heptylquinoline (HHQ) and 2-heptyl-3,4-dihydroxyquinoline (pseudomonas quinolone signal, PQS). This method does not depend on the redox activity of the QS molecules. Instead, as a proof-of-concept, electrochemical monitoring was achieved through aqueous alkali metal ion and proton interfacial complexation with organic-solubilized HHQ and PQS at an interface between two immiscible electrolytic solutions, specifically, between water and 1,2-dichloroethane. The proton/HHQ and proton/PQS binding stoichiometries were discovered to be 1:3 and 1:2, respectively, which is likely due to the relatively high concentrations of QS molecules employed. Owing to the biphasic nature of the methodology, experimental complications due to the poor solubility of the hydrophobic QS molecules in aqueous media were avoided.

show abstract

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection ofPseudomonas aeruginosaQuorum Sensing Molecules at a Liquid|Liquid Interface

et al. 2019

View full text Add to dashboard Cite

show abstract

“…This setup could regenerate reductive pyocyanin from its oxidative state and this way enable repeated pyocyanin registration. With this method it was possible to reach an LOD of 47 pM [ 43 ].…”

Section: Electrochemical Detection Of Pyocyaninmentioning

confidence: 99%

“…To successfully apply the electrochemical detection of pyocyanin for PA identification in clinical samples, several studies measured human fluids spiked with pyocyanin [ 31 , 32 , 48 ]. The investigated human fluids covered urine, blood, bronchial lavage, sputum, saliva and hypertonic saline for laryngeal aspirate suctions as well as simulated wound fluid and artificial sputum medium [ 25 , 33 , 37 , 42 , 43 ]. PA is a urinary tract pathogen, which makes urine an obvious body fluid to develop pyocyanin detection in.…”

Section: Towards Clinical Applicationsmentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical Detection of Pyocyanin as a Biomarker for Pseudomonas aeruginosa: A Focused Review

Alatraktchi

Svendsen

Molin

2020

Sensors

View full text Add to dashboard Cite

Pseudomonas aeruginosa (PA) is a pathogen that is recognized for its advanced antibiotic resistance and its association with serious diseases such as ventilator-associated pneumonia and cystic fibrosis. The ability to rapidly detect the presence of pathogenic bacteria in patient samples is crucial for the immediate eradication of the infection. Pyocyanin is one of PA’s virulence factors used to establish infections. Pyocyanin promotes virulence by interfering in numerous cellular functions in host cells due to its redox-activity. Fortunately, the redox-active nature of pyocyanin makes it ideal for detection with simple electrochemical techniques without sample pretreatment or sensor functionalization. The previous decade has seen an increased interest in the electrochemical detection of pyocyanin either as an indicator of the presence of PA in samples or as a tool for quantifying PA virulence. This review provides the first overview of the advances in electrochemical detection of pyocyanin and offers an input regarding the future directions in the field.

show abstract

“…Recently, several studies have demonstrated applications of the PADs to different types of food and environmental samples using detection schemes such as colorimetry, − electrochemistry, − fluorometry, − and chemiluminescence. − Colorimetry is the most frequently employed detection method because it is simple to achieve qualitative and quantitative analyses of analytes by observing the color change in the detection zones on the PAD. The color images are captured with a digital camera, smartphone, or scanner, which then is followed by image processing using software such as ImageJ. , …”

Section: Introductionmentioning

confidence: 99%

Development of Pipetteless Paper-Based Analytical Devices with a Volume Gauge

et al. 2023

View full text Add to dashboard Cite

In this work, we propose a new design for paperbased analytical devices (PADs) that eliminate the need to use a micropipette for sample introduction. With this design, a PAD is equipped with a distance-based detection channel that is connected to a storage channel that indicates the volume of a sample introduced into the PAD. The analyte in the sample solution reacts with a colorimetric reagent deposited into the distance-based detection channel as the sample solution flows into the storage channel where the volume is measured. The ratio of the lengths of the detection channel and that of the storage channel (D/S ratio) are constant for a sample containing a certain concentration, which is independent of the introduced volume. Therefore, the PADs permit volume-independent quantification using a dropper instead of a micropipette because the length of the storage channel plays the role of a volume gauge to estimate the introduced sample volume. In this study, the D/S ratios obtained with a dropper were comparable to those obtained with a micropipette, which confirmed that precise volume control is unnecessary for this PAD system. The proposed PADs were applied to the determinations of iron and bovine serum albumin using bathophenanthroline and tetrabromophenol blue as colorimetric reagents, respectively. The calibration curves showed good linear relationships with coefficients of 0.989 for iron and 0.994 for bovine serum albumin, respectively.

show abstract

Submicromolar quantification of pyocyanin in complex biological fluids using pad-printed carbon electrodes

Cited by 14 publications

References 20 publications

Electrochemical Detection ofPseudomonas aeruginosaQuorum Sensing Molecules at a Liquid|Liquid Interface

Electrochemical Detection ofPseudomonas aeruginosaQuorum Sensing Molecules at a Liquid|Liquid Interface

Electrochemical Detection of Pyocyanin as a Biomarker for Pseudomonas aeruginosa: A Focused Review

Development of Pipetteless Paper-Based Analytical Devices with a Volume Gauge

Contact Info

Product

Resources

About