Sublethal injury and virulence changes in Listeria monocytogenes and Listeria innocua treated with antimicrobials carvacrol and citral

Silva, A.; Genovés, Salvador; Martorell, Patricia; Zanini, Surama Freitas; Rodrigo, Dolores; Martínez, Antonio

doi:10.1016/j.fm.2015.02.016

Cited by 28 publications

(22 citation statements)

References 34 publications

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“…It is worth mentioning that researchers recently showed that 10-day consecutive selection with citral at SIC induces genotypic resistant E. coli strains 51 and exposure to citral may enhance bacterial virulence of Listeria spp. in a Caenorhabditis elegans model 52 . Although resistant mutant was not observed in our trial experiments, further research is warranted to test this possibility to determine the appropriate application of the citral, either being developed as an alternative/supplementary strategy to control C. sakazakii infections or as a novel hurdle in food preservation in combination with other preservative technologies.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Cronobacter sakazakii Virulence Factors by Citral

Shi

Sun

Liu

et al. 2017

Sci Rep

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Cronobacter sakazakii is a foodborne pathogen associated with fatal forms of necrotizing enterocolitis, meningitis and sepsis in neonates and infants. The aim of this study was to determine whether citral, a major component of lemongrass oil, could suppress putative virulence factors of C. sakazakii that contribute to infection. Sub-inhibitory concentrations of citral significantly decreased motility, quorum sensing, biofilm formation and endotoxin production. Citral substantially reduced the adhesion and invasion of C. sakazakii to Caco-2 cells and decreased bacterial survival and replication within the RAW 264.7 macrophage cells. Citral also repressed the expression of eighteen genes involved in the virulence. These findings suggest that citral has potential to be developed as an alternative or supplemental agent to mitigate the infections caused by C. sakazakii.

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Section: Discussionmentioning

confidence: 99%

Inhibition of Cronobacter sakazakii Virulence Factors by Citral

Shi

Sun

Liu

et al. 2017

Sci Rep

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“…et al, 2015; Rajalaxmi et al, 2016). Natural compounds that affected the virulence of Gram-positive Clostridium difficile (Yun et al, 2015) and Listeria monocytogenes (Silva et al, 2015; Sivaranjani et al, 2016) have also been recently described.…”

Section: Identification Of Novel Anti-infectivesmentioning

confidence: 99%

Beyond Traditional Antimicrobials: A Caenorhabditis elegans Model for Discovery of Novel Anti-infectives

et al. 2016

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The spread of antibiotic resistance amongst bacterial pathogens has led to an urgent need for new antimicrobial compounds with novel modes of action that minimize the potential for drug resistance. To date, the development of new antimicrobial drugs is still lagging far behind the rising demand, partly owing to the absence of an effective screening platform. Over the last decade, the nematode Caenorhabditis elegans has been incorporated as a whole animal screening platform for antimicrobials. This development is taking advantage of the vast knowledge on worm physiology and how it interacts with bacterial and fungal pathogens. In addition to allowing for in vivo selection of compounds with promising anti-microbial properties, the whole animal C. elegans screening system has also permitted the discovery of novel compounds targeting infection processes that only manifest during the course of pathogen infection of the host. Another advantage of using C. elegans in the search for new antimicrobials is that the worm itself is a source of potential antimicrobial effectors which constitute part of its immune defense response to thwart infections. This has led to the evaluation of effector molecules, particularly antimicrobial proteins and peptides (APPs), as candidates for further development as therapeutic agents. In this review, we provide an overview on use of the C. elegans model for identification of novel anti-infectives. We highlight some highly potential lead compounds obtained from C. elegans-based screens, particularly those that target bacterial virulence or host defense to eradicate infections, a mechanism distinct from the action of conventional antibiotics. We also review the prospect of using C. elegans APPs as an antimicrobial strategy to treat infections.

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“…Its body is transparent which allows observation of most of internal organs and most of the main physiological processes and stressed responses of higher organisms like those that humans are preserved in C. elegans. It is easy and economical to use in optimal laboratory conditions due to its short lifespan and it has been successfully employed in some studies (Chai-Hoon et al, 2010;Silva et al, 2015;Ewbank et al, 2011).…”

Section: Introductionmentioning

confidence: 99%