Resistance changes in Salmonella enterica serovar Typhimurium treated by High Hydrostatic Pressure and Pulsed Electric Fields and assessment of virulence changes by using Caenorhabditis elegans as a test organism

Sanz-Puig, Maria; Velázquez-Moreira, Adriana; Torres, Clara; Guerrero-Beltrán, José Ángel; Cunha, Luís Miguel; Martínez, Antonio; Rodrigo, Dolores

doi:10.1016/j.ifset.2018.04.002

Cited by 10 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Additional studies are also demonstrated the development of resistance when S. enterica var Typhimurium is treated with non-essential oil natural antimicrobials (polyphenols) (Gupta and Birdi, 2017). Under other sub-lethal physical treatments, like Pulsed Electric Fields and High Hydrostatic Pressure, S. enterica var Typhimurium has also demonstrated ability to be adapted (Sanz-Puig et al, 2018) In order to further understand the mechanism of resistant bacteria infection, and the possible evident changes in virulence due to this adaptation step in relation to untreated bacteria, the C. elegans in vivo model was used. Up to date, over human pathogens have been analyzed in the C. elegans infection model (Battisti et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Occurrence of Salmonella typhimurium resistance under sublethal/repeated exposure to cauliflower infusion and infection effects on Caernohabditis elegans host test organism

Sanz-Puig

Arana-Lozano

Pina-Pérez

et al. 2019

Food sci. technol. int.

Self Cite

View full text Add to dashboard Cite

Resistant bacteria to antimicrobials are increasingly emerging in medical, food industry and livestock environments. The present research work assesses the capability of Salmonella enterica var Typhimurium to become adapted under the exposure to a natural cauliflower antimicrobial by-product infusion in consecutive repeated exposure cycles. Caenorhabditis elegans was proposed as in vivo host-test organism to compare possible changes in the virulent pattern of the different rounds treated S. enterica var Typhimurium and untreated bacterial cells. According to the obtained results, S. enterica var Typhimurium was able to generate resistance against a repeated exposure to cauliflower by-product infusion 5% (w/v), increasing the resistance with the number of exposed repetitions. Meanwhile, at the first exposure, cauliflower by-product infusion was effective in reducing S. enterica var Typhimurium (≈1 log10 cycle), and S. enterica var Typhimurium became resistant to this natural antimicrobial after the second and third treatment-round and was able to grow (≈1 log10 cycle). In spite of the increased resistance observed for repeatedly treated bacteria, the present study reveals no changes on C. elegans infection effects between resistant and untreated S. enterica var Typhimurium, according to phenotypic parameters evaluation (lifespan duration and egg-laying).

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Occurrence of Salmonella typhimurium resistance under sublethal/repeated exposure to cauliflower infusion and infection effects on Caernohabditis elegans host test organism

Sanz-Puig

Arana-Lozano

Pina-Pérez

et al. 2019

Food sci. technol. int.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Typhimurium) were provided by the Spanish Type Culture Collection. Following the procedure described by Sanz-Puig et al [ 35 ], lyophilized samples (0.5 g lyophil) were rehydrated in Tryptone Soy Broth (TSB) (Scharlab S.A., Barcelona, Spain) ( L . monocytogenes and S .…”

Section: Methodsmentioning

confidence: 99%

“…Pure cultures of Listeria monocytogenes serovar 4b (Spanish Type Culture Collection (CECT) 4032) (L. monocytogenes), Escherichia coli O157:H7 (CECT 5947) (E. coli) and Salmonella enterica serovar Typhimurium (CECT 443) (S. Typhimurium) were provided by the Spanish Type Culture Collection. Following the procedure described by Sanz-Puig et al [35], lyophilized samples (0.5 g lyophil) were rehydrated in Tryptone Soy Broth (TSB) (Scharlab S.A., Barcelona, Spain) (L. monocytogenes and S. Typhimurium) or in Luria Broth (LB) (Scharlab S.A., Barcelona, Spain) (E. coli). Rehydrated cultures were transferred to 500 mL of its corresponding media and incubated in a bath shaker (200 rpm) for 14 h at 37˚C.…”

Section: Microbial Strainsmentioning

confidence: 99%

Antimicrobial effect of insect chitosan on Salmonella Typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes survival

et al. 2020

Self Cite

View full text Add to dashboard Cite

The antimicrobial capability of chitosan from Tenebrio molitor as compared with chitosan from crustacean (Penaeus monodon) on different pathogenic microorganisms of concern in food safety was studied. The antimicrobial effect was tested at pH 5 and pH 6.2 and at two different initial concentrations (103 or 106 CFU/mL). Results indicated that chitosan from both sources have antimicrobial activity, although the effect depended on the microorganism considered (Salmonella Typhimurium, Listeria monocytogenes and Escherichia coli O157:H7). Our results indicated that Salmonella was the most resistant bacteria, and that chitosan from insect was less active than chitosan from crustacean, especially against Salmonella. Another important factor on antimicrobial activity was the pH of the sample. When chitosan was added to a solution with a pH of 6.2 it was more active against Listeria and Escherichia coli, than at pH 5.00. Besides, the effect of chitosan appears to decrease with the incubation time, since some increases in counts were observed on E. coli and Salmonella after the 24 and 49 hours of incubation.

show abstract

“…Salmonella enterica serovar Typhimurium and Cronobacter sakazakii are foodborne pathogens that are highly relevant to the food processing industry (Endersen et al, 2017;Sanz-Puig et al, 2018). In 2016, 300,524 cases of foodborne outbreaks were reported in Europe, of which 26.22% were caused by Salmonella spp.…”

Section: Introductionmentioning

confidence: 99%

Removal of Salmonella enterica serovar Typhimurium and Cronobacter sakazakii biofilms from food contact surfaces through enzymatic catalysis

Ripolles‐Avila

Ríos-Castillo

Fontecha‐Umaña

et al. 2019

Journal of Food Safety

View full text Add to dashboard Cite

Bacterial biofilms are highly difficult to control, hence significant economic resources have been allocated to develop strategies to eradicate them. This study evaluated the effect of an enzymatic treatment to be used as a cleaning product to control the presence of biofilms. Two different materials used in the food industry, polystyrene and stainless steel, were tested using Salmonella Typhimuirum and Cronobacter sakazakii. Biofilm formation was carried out by inoculating the surfaces with a standardized concentration of 4 log (CFU cm−2) and incubated for 48 hr with renewal of nutrients. The biofilm formation and subsequent enzymatic treatment were quantified using fluorescence microscopy and the conventional culture method. The enzymatic treatment showed significant reductions of 2–3 log (CFU cm−2) in biofilm cells, which was attributed to the degradation of the extracellular matrix and the further detachment of both microorganisms. The maximum biofilm detachment obtained with the preventive formula was 46.67%; however, this percentage could be increased by applying an aggressive treatment or by adding a subsequent disinfection step that would eliminate adhered microbial cells. Further, the enzymatic cleaning treatment could be exploited as a potent technology to control bacterial adherence and biofilm formation in the food industry.

show abstract

Resistance changes in Salmonella enterica serovar Typhimurium treated by High Hydrostatic Pressure and Pulsed Electric Fields and assessment of virulence changes by using Caenorhabditis elegans as a test organism

Abstract: Resistance changes in Salmonella enterica serovar Typhimurium treated by high hydrostatic pressure and pulsed electric fields and assessment of virulence changes by using Caenorhabditis elegans as a test organism

Cited by 10 publications

References 36 publications

Occurrence of Salmonella typhimurium resistance under sublethal/repeated exposure to cauliflower infusion and infection effects on Caernohabditis elegans host test organism

Occurrence of Salmonella typhimurium resistance under sublethal/repeated exposure to cauliflower infusion and infection effects on Caernohabditis elegans host test organism

Antimicrobial effect of insect chitosan on Salmonella Typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes survival

Removal of Salmonella enterica serovar Typhimurium and Cronobacter sakazakii biofilms from food contact surfaces through enzymatic catalysis

Contact Info

Product

Resources

About