2012
DOI: 10.1038/ncb2591
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Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material

Abstract: The centrosome is the main microtubule organization centre of animal cells. It is composed of a centriole pair surrounded by pericentriolar material (PCM). Traditionally described as amorphous, the architecture of the PCM is not known, although its intricate mode of assembly alludes to the presence of a functional, hierarchical structure. Here we used subdiffraction imaging to reveal organizational features of the PCM. Interphase PCM components adopt a concentric toroidal distribution of discrete diameter arou… Show more

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Cited by 336 publications
(436 citation statements)
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“…12,13 Surrounding the centrioles, the interphase PCM is supported by a structured series of concentricallyarranged toroidal rings rich in coiled-coil domain scaffold proteins such as Pericentrin, Cep192, and CDK5RAP2. 14 While the substructure of the mitotic PCM is less clear, it has been demonstrated that its maturation is cued by phosphorylation of Pericentrin by the mitotic kinase Plk1 to allow further recruitment of Cep192, g-tubulin, and other core components. 12 Properly separated mature centrosomes organize the bipolar mitotic spindle to ensure proper chromosome segregation and successful cell division.…”
Section: Centrosome Activity and Behavior Changes With The Cell Cyclementioning
confidence: 99%
“…12,13 Surrounding the centrioles, the interphase PCM is supported by a structured series of concentricallyarranged toroidal rings rich in coiled-coil domain scaffold proteins such as Pericentrin, Cep192, and CDK5RAP2. 14 While the substructure of the mitotic PCM is less clear, it has been demonstrated that its maturation is cued by phosphorylation of Pericentrin by the mitotic kinase Plk1 to allow further recruitment of Cep192, g-tubulin, and other core components. 12 Properly separated mature centrosomes organize the bipolar mitotic spindle to ensure proper chromosome segregation and successful cell division.…”
Section: Centrosome Activity and Behavior Changes With The Cell Cyclementioning
confidence: 99%
“…In animal cells, the centrosome is composed of two centrioles surrounded by the pericentriolar material (PCM). The PCM has a precise organization, which derives from the hierarchical recruitment of a small number of large coiled-coil proteins around the centrioles [3,4]. The centrioles ensure the stability and the duplication of the centrosome, whereas the PCM anchors microtubule-nucleating complexes and cell cycle regulators.…”
Section: Introductionmentioning
confidence: 99%
“…17,18 Indeed, several reports have lately revealed the presence of functional domains within the PCM, defined as concentric layers enclosing the mature centriole in a tube-or toroid-like arrangement. [19][20][21] The outer PCM layer contains proteins involved in microtubule nucleation such as g-tubulin. 20 Pericentrin adopts an extended conformation and is organized radially with its C terminus associated to the centriole wall and its N terminus extending toward the PCM periphery.…”
Section: Introductionmentioning
confidence: 99%
“…[19][20][21] The outer PCM layer contains proteins involved in microtubule nucleation such as g-tubulin. 20 Pericentrin adopts an extended conformation and is organized radially with its C terminus associated to the centriole wall and its N terminus extending toward the PCM periphery. 20 Microtubule nucleation is initiated by protein complexes containing g-tubulin and called gTuRC (g-Tubulin Ring Complexes).…”
Section: Introductionmentioning
confidence: 99%
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