Subcutaneous Phaeohyphomycotic Nodule Due to Phialemoniopsis hongkongensis sp. nov

Tsang, Chi‐Ching; Chan, Jasper Fuk‐Woo; Ip, Philip; Ngan, Antonio H. Y.; Chen, Jonathan Hon-Kwan; Lau, Susanna K.P.; Woo, Patrick C. Y.

doi:10.1128/jcm.01592-14

Cited by 21 publications

(20 citation statements)

References 33 publications

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“…Since E. anophelis is not included in the Bruker reference, mass spectra generated from seven E. anophelis strains with identity confirmed by 16S rRNA gene sequencing were later added to the database. Obtained spectra were subject to hierarchical cluster analysis as described previously 24 . Antibiotic susceptibility testing was performed by Kirby Bauer disk diffusion method with results interpreted according to Clinical and Laboratory Standards Institute for Staphylococcus aureus (vancomycin) and Pseudomonas aeruginosa (other drugs), because of the lack of interpretative criteria for Elizabethkingia 25 .…”

Section: Methodsmentioning

confidence: 99%

Elizabethkingia anophelis bacteremia is associated with clinically significant infections and high mortality

Lau

Chow

Foo

et al. 2016

Sci Rep

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Unlike Elizabethkingia meningoseptica, the clinical importance of E. anophelis is poorly understood. We determined the clinical and molecular epidemiology of bacteremia caused by Elizabethkingia-like species from five regional hospitals in Hong Kong. Among 45 episodes of Elizabethkingia-like bacteremia, 21 were caused by Elizabethkingia, including 17 E. anophelis, three E. meningoseptica and one E. miricola; while 24 were caused by other diverse genera/species, as determined by 16S rRNA gene sequencing. Of the 17 cases of E. anophelis bacteremia, 15 (88%) were clinically significant. The most common diagnosis was pneumonia (n = 5), followed by catheter-related bacteremia (n = 4), neonatal meningitis (n = 3), nosocomial bacteremia (n = 2) and neutropenic fever (n = 1). E. anophelis bacteremia was commonly associated with complications and carried 23.5% mortality. In contrast, of the 24 episodes of bacteremia due to non-Elizabethkingia species, 16 (67%) were clinically insignificant. Compared to non-Elizabethkingia bacteremia, Elizabethkingia bacteremia was associated with more clinically significant infections (P < 0.01) and positive cultures from other sites (P < 0.01), less polymicrobial bacteremia (P < 0.01), and higher complication (P < 0.05) and mortality (P < 0.05) rates. Elizabethkingia bacteremia is predominantly caused by E. anophelis instead of E. meningoseptica. Elizabethkingia bacteremia, especially due to E. anophelis, carries significant morbidity and mortality, and should be considered clinically significant unless proven otherwise.

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Section: Methodsmentioning

confidence: 99%

Elizabethkingia anophelis bacteremia is associated with clinically significant infections and high mortality

Lau

Chow

Foo

et al. 2016

Sci Rep

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165

237

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“…Phialemoniopsis is a new genus established by Perdomo et al (4) after phylogenetic investigation of the unidentified clinical isolates of Acremonium, Phiale monium and Lecythophora by detailed morphological and molecular analyses (4,5). The genus includes 5 species: P. ocularis (=Srcopodium oculorum), P. curvata (=Phialemonium curvata), P. cornearis, P. pluriloculosa and P. hongkongensis (4,6). These species are widely distributed in various environments, including air, indu-strial water, plant materials, river water, sewage and soil (4).…”

Section: Discussionmentioning

confidence: 99%

“…Four cases of skin infections caused by Phialemoni opsis have been reported previously (6-9). P. curvata (7,8), P. hongkongensis (6) and P. ocularis (9) were isolated from nodules on the foot (7-9) or forearm (6), and the patients were treated with surgical debridement (8), amphotericin B and ketoconazole with debridement (7), itraconazole (6) or voriconazole (9). Although 2 of the patients were immunocompromised (7,8), none had systemic involvement.…”

Section: Discussionmentioning

confidence: 99%

Concurrent Double Fungal Infections of the Skin Caused by Phialemoniopsis endophytica and Exophiala jeanselmei in a Patient with Microscopic Polyangiitis

Ito¹,

Yamada²,

Kimura³

et al. 2017

Acta Derm Venerol

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“…255343, Bruker Daltonics) was used for calibration and quality control in each run. Obtained spectra were subject to hierarchical cluster analysis using ClinProTools 3.0 (Bruker Daltonics) as described previously [ 43 ].…”

Section: Methodsmentioning

confidence: 99%

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for rapid identification of mold and yeast cultures of Penicillium marneffei

et al. 2016

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BackgroundPenicillium marneffei is the most important thermal dimorphic fungus causing systemic mycosis in HIV-infected and other immunocompromised patients in Southeast Asia. However, laboratory diagnosis of penicilliosis, which relies on microscopic morphology and mycelial-to-yeast conversion, is time-consuming and expertise-dependent, thus delaying diagnosis and treatment. Although matrix -assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is useful for identification of various medically important fungi, its performance for identification of P. marneffei is less clear.ResultsWe evaluated the performance of the Bruker MALDI-TOF MS system for identification of mold and yeast cultures of 59 clinical strains and the type strain of P. marneffei using the direct transfer method, with results compared to four phylogenetically closely related species, P. brevi-compactum, P. chrysogenum, Talaromyces aurantiacus and T. stipitatus. Using the Bruker original database combined with BDAL v4.0.0.1 and Filamentous Fungi Library 1.0, MALDI-TOF MS failed to identify the 60 P. marneffei strains grown in mold and yeast phase (identified as P. funiculosum and P. purpurogenum with scores <1.7 respectively). However, when the combined database was expanded with inclusion of spectra from 21 P. marneffei strains in mold and/or yeast phase, all the remaining 39 P. marneffei strains grown in mold or phase were correctly identified to the species level with score >2.0. The MS spectra of P. marneffei exhibited significant difference to those of P. brevi-compactum, P. chrysogenum, T. aurantiacus and T. stipitatus. However, MALDI-TOF MS failed to identify these four fungi to the species level using the combined database with or without spectra from P. marneffei.ConclusionsMALDI-TOF MS is useful for rapid identification of both yeast and mold cultures of P. marneffei and differentiation from related species. However, accurate identification to the species level requires database expansion using P. marneffei strains.

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Subcutaneous Phaeohyphomycotic Nodule Due to Phialemoniopsis hongkongensis sp. nov

Cited by 21 publications

References 33 publications

Elizabethkingia anophelis bacteremia is associated with clinically significant infections and high mortality

Elizabethkingia anophelis bacteremia is associated with clinically significant infections and high mortality

Concurrent Double Fungal Infections of the Skin Caused by Phialemoniopsis endophytica and Exophiala jeanselmei in a Patient with Microscopic Polyangiitis

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for rapid identification of mold and yeast cultures of Penicillium marneffei

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