Subcutaneous P<scp>o</scp><sub>2</sub> as an index of the physiological limits for hemodilution in the rat

Pearce, Frederick J.; Waasdorp, C.; Hufnagel, Howard; Burris, David; DeFeo, Joseph A.; Soballe, Peter W.; Drucker, William R.

doi:10.1152/japplphysiol.00816.2003

Cited by 6 publications

(1 citation statement)

References 61 publications

(68 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ANH was performed by withdrawal of blood at a rate of 0.5 to 1 mL/min during simultaneous infusion of the same amount of warmed HES solution (Voluven ; Fresenius, Bad Homburg, Germany). The amount of blood withdrawn during hemodilution was calculated using the following formula [10]: V = EBV × (Hi − Hf) ÷ Hav, where V is the volume of blood to be removed, EBV is the estimated blood volume (7% of body weight in rat) [11], Hi is the initial hematocrit level before the onset of hemodilution, Hf is the desired hematocrit level at the end of hemodilution, and Hav is the average hematocrit level during hemodilution (average of Hi plus Hf). The desired hemoglobin concentration at the end of hemodilution was 7.0 g/dl.…”

Section: Hemodilutionmentioning

confidence: 99%

The Effects of Hemodilution on Acute Inflammatory Responses in a Bleomycin-Induced Lung Injury Model

Cheong

Lee

et al. 2009

Experimental Lung Research

View full text Add to dashboard Cite

Acute normovolemic hemodilution (ANH) can be used in acute lung injury (ALI) patients who refuse blood transfusions. To investigate the effects of hemodilution on the acute inflammatory response in lung injury, the authors studied the effects of ANH in a rat model of bleomycin-induced lung injury. Bleomycin (10 mg/kg) was used to induce lung injury in 2 groups of rats. The treatment groups included a lung injury group with hemodilution (HI), a lung injury group without hemodilution (NHI), and a control group. Hemodilution was performed by removing blood and substituting the same amount of hydroxyethyl starch solution targeted to 7.0 g/dL via the right and left internal jugular veins. At day 3 after bleomycin instillation, systemic hemoglobin concentration was 9.5 +/- 1.1 g/dL. Tumor necrosis factor-alpha, interleukin-1beta, and interleukin-6 levels measured in the bronchoalveolar lavage fluid (BALF), blood, and lung tissue were not significantly different between the HI and NHI groups 3 days after lung injury. Microscopic findings showed fibrosis and inflammation in the HI and NHI groups 28 days after lung injury, but no significant differences were found between the 2 groups. Hemodilution after bleomycin administration did not further affect the acute inflammatory response or lung injury.

show abstract

Section: Hemodilutionmentioning

confidence: 99%

The Effects of Hemodilution on Acute Inflammatory Responses in a Bleomycin-Induced Lung Injury Model

Cheong

Lee

et al. 2009

Experimental Lung Research

View full text Add to dashboard Cite

show abstract

The Application of Novel Optical Sensors (Optodes) in Experimental Plant Ecology

Gansert

Bloßfeld

2008

Progress in Botany

View full text Add to dashboard Cite

Oxygen tension in rat cerebral cortex microvessels in acute anemia

Vovenko

Chuikin

2008

Neurosci Behav Physi

View full text Add to dashboard Cite

Polarigraphic microelectrodes were used to study the distribution of oxygen tension (pO(2)) in arterioles (lumen diameters 8-80 microm) and venules (lumen diameters 8-120 microm) in the rat cerebral cortex during acute reductions in blood hemoglobin ([Hb]). Isovolumic hemodilution with 5% albumin solution was performed in steps from an initial [Hb] of 14.1 +/- 0.3 g/dl (control) to 9.8 +/- 0.3 g/dl (step 1), 6.6 +/- 0.4 g/dl (step 2), and 4.6 +/- 0.3 g/dl (step 3). Mild anemia (step 1, hematocrit 30%) led to an increase in pO(2) in the arterial side of the microcirculatory bed, with virtually no change in pO(2) in the venous side. Step 2 (hematocrit 20%) was accompanied by a further insignificant increase in pO(2) in arterioles, while there was a significant reduction (on average to 32 mmHg) in venules. Step 3 (hematocrit 13-14%) led to a (statistically insignificant) increase in pO(2) in arterioles. pO(2) in venules decreased, on average, to 27 mmHg; the proportion of smallest venules with low pO(2) values (less than 20 mmHg) increased to 31% (from 3% in controls). In some capillaries, pO(2) was 5-10 mmHg, which was an indicator of the presence of hypoxic zones in brain tissues. These zones primarily arose close to the smallest capillary and venous microvessels, with slowed or impaired blood flow.

show abstract

Subcutaneous Po₂ as an index of the physiological limits for hemodilution in the rat

Abstract: . Subcutaneous PO2 as an index of the physiological limits for hemodilution in the rat.

Cited by 6 publications

References 61 publications

The Effects of Hemodilution on Acute Inflammatory Responses in a Bleomycin-Induced Lung Injury Model

The Effects of Hemodilution on Acute Inflammatory Responses in a Bleomycin-Induced Lung Injury Model

The Application of Novel Optical Sensors (Optodes) in Experimental Plant Ecology

Oxygen tension in rat cerebral cortex microvessels in acute anemia

Contact Info

Product

Resources

About

Subcutaneous Po2 as an index of the physiological limits for hemodilution in the rat

Abstract: . Subcutaneous PO2 as an index of the physiological limits for hemodilution in the rat.

Cited by 6 publications

References 61 publications

The Effects of Hemodilution on Acute Inflammatory Responses in a Bleomycin-Induced Lung Injury Model

The Effects of Hemodilution on Acute Inflammatory Responses in a Bleomycin-Induced Lung Injury Model

The Application of Novel Optical Sensors (Optodes) in Experimental Plant Ecology

Oxygen tension in rat cerebral cortex microvessels in acute anemia

Contact Info

Product

Resources

About

Subcutaneous Po₂ as an index of the physiological limits for hemodilution in the rat