The Application of Novel Optical Sensors (Optodes) in Experimental Plant Ecology

Gansert, Dirk; Bloßfeld, Stephan

doi:10.1007/978-3-540-72954-9_14

Cited by 14 publications

(7 citation statements)

References 119 publications

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“…Subsequently, each pixel in the recorded image can be ascribed an oxygen concentration value converting the recorded lifetimes to oxygen concentrations using the modified Stern-Volmer equation (Holst and Grunwald 2001). For a general introduction to the use of planar optodes for quantitative imaging in root studies, we refer to Blossfeld and Gansert (2012) and Gansert and Blossfeld (2008).…”

Section: Planar Optode Studies Of Rhizosphere Oxygen Dynamicsmentioning

confidence: 99%

“…Planar optode technology facilitates recording of oxygen distribution in sediments and soils as two-dimensional images (Glud et al 1996;Holst et al 1998) and is increasingly being used for investigations of plant-mediated sediment oxygenation at depth in waterlogged rhizospheres (Blossfeld 2013;Blossfeld and Gansert 2012;Gansert and Blossfeld 2008). Even so, its use in wetlands and shallow waters is still limited to very few species including Juncus spp.…”

Section: Introductionmentioning

confidence: 99%

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The Dynamics of Plant-Mediated Sediment Oxygenation in Spartina anglica Rhizospheres—a Planar Optode Study

Koop-Jakobsen

Wenzhöfer

2014

Estuaries and Coasts

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Belowground sediment oxygenation in rhizospheres of wetland plants promotes nutrient uptake, serve as protection against toxic reduced compounds and play an important role in wetland nutrient cycling. The presence of 1.5-mm-wide oxic zones around roots of the intertidal marsh grass Spartina anglica was demonstrated below the sediment surface using planar optode technology recording 2D images of the sediment oxygen distribution. Oxic root zones were restricted to the root tips stretching up to 16 mm along the roots with an oxygen concentration up to 85 μmol L −1 detected at the root surface. Radial oxygen loss across the root surface ranged from 250 to 300 nmol m −2 s −1 , which is comparable to other wetland plants. During air exposure of the aboveground biomass, atmospheric oxygen was the primary source for belowground oxygen transport, and light availability only had a minor effect on the belowground sediment oxygenation. During inundations completely submerging the aboveground biomass cutting off access to atmospheric oxygen, oxic root zones diminished significantly in the light and were completely eliminated in darkness. Within the time frame of a normal tidal inundation (~1.5 h), photosynthetic oxygen production maintained the presence of oxic root zones in light, whereas oxic root zones were eliminated within 1 h in darkness. The results show that the sediment oxygenation in Spartina anglica rhizospheres is temporally dynamic as well as spatially variable along the roots.

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Section: Planar Optode Studies Of Rhizosphere Oxygen Dynamicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Dynamics of Plant-Mediated Sediment Oxygenation in Spartina anglica Rhizospheres—a Planar Optode Study

Koop-Jakobsen

Wenzhöfer

2014

Estuaries and Coasts

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“…This technique uses planar pH optodes, based on the measurement of the fluorescence decay time of pH sensitive indicator dyes (Gansert and Blossfeld 2008;Gansert et al 2006;Huber et al 2001;Klimant et al 2001). These indicator dyes are dispersed and immobilized on an inert supporting film, creating a thin sensor foil of 10 µm in thickness.…”

Section: Plant Species and Cultivationmentioning

confidence: 99%

Rhizosphere pH dynamics in trace-metal-contaminated soils, monitored with planar pH optodes

Bloßfeld

Perriguey²,

Sterckeman³

et al. 2009

Plant Soil

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The present study presents new insights into pH dynamics in the rhizosphere of alpine pennycress (Noccaea caerulescens (J. Presl & C. Presl) F.K. Mey), maize (Zea mays L.) and ryegrass (Lolium perenne L.), when growing on three soils contaminated by trace metals with initial pH values varying from 5.6 to 7.4. The pH dynamics were recorded, using a recently developed 2D imaging technique based on planar pH optodes. This showed that alpine pennycress and ryegrass alkalinized their rhizosphere by up to 1.7 and 1.5 pH units, respectively, whereas maize acidified its rhizosphere by up to −0.7 pH units. The alkalinization by the roots of alpine pennycress and ryegrass was permanent and not restricted to specific root zones, whereas the acidification along the maize roots was restricted to the elongation zone and thus only temporary. Calculations showed that such pH changes should have noticeable effects on the solubility of the trace metal in the rhizosphere, and therefore on their uptake by the plants. As a result, it is suggested that models for trace metal uptake should include precise knowledge of rhizospheric pH conditions.

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“…The pH and O 2 measurements were made using planar optodes (PRESENS, Regensburg, Germany), a noninvasive optical technique (Blossfeld and Gansert, 2007). This measurement is based on different fluorescence decay times of pH-or O 2 -sensitive indicator dyes compared to a reference (Gansert and Blossfeld, 2008). An optical glass fiber, connected to a light source and measuring device (pH-1 mini, PRESENS, Regensburg, Germany) conveys the excitation light pulse (470 nm).…”

Section: Planar Optodesmentioning

confidence: 99%

Monitoring rhizospheric pH, oxygen, and organic acid dynamics in two short‐time flooded plant species

Schreiber

Zeng

Bloßfeld³

et al. 2012

Z. Pflanzenernähr. Bodenk.

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The rhizosphere of two flooding-resistant plant species (Arundinella anomala Steud., Alternanthera philoxeroides Mart.) from Three Gorges Reservoir area (China) has been examined for reactions to waterlogging and submergence. Rhizosphere parameters were monitored in natural sediment substrate by means of a dual-access floodable rhizobox, which allows monitoring of oxygen and pH dynamics noninvasively with planar optodes in high temporal and spatial resolution, as well as simultaneous low-invasive soil-solution sampling. Analysis of samples for low-molecular-weight organic acids (LMWOA) was done by capillary electrophoresis. Roots could be observed easily in situ during growth and exposure to flooding. The floodable rhizobox is therefore considered a valuable tool for root-reaction monitoring also under flooding conditions. During waterlogging, both species exuded oxygen into their rhizosphere and showed diurnal rhythms of rhizospheric acidification. The pH of the rhizosphere of growing root tips decreased up to 0.8 units corresponding to higher LMWOA concentrations. These rhythms weakened during flooding, but gained maximum amplitude again rapidly after resurfacing. We conclude that the root system was still fully functioning during and after flooding, and that flooding poses no threat to the physiology of the root system of the study species

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The Application of Novel Optical Sensors (Optodes) in Experimental Plant Ecology

Cited by 14 publications

References 119 publications

The Dynamics of Plant-Mediated Sediment Oxygenation in Spartina anglica Rhizospheres—a Planar Optode Study

The Dynamics of Plant-Mediated Sediment Oxygenation in Spartina anglica Rhizospheres—a Planar Optode Study

Rhizosphere pH dynamics in trace-metal-contaminated soils, monitored with planar pH optodes

Monitoring rhizospheric pH, oxygen, and organic acid dynamics in two short‐time flooded plant species

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