Subcutaneous implants coated with tissue‐engineered cartilage

Kim, Soo Whan; Dobratz, Eric J.; Ballert, John A.; Voglewede, Andrew; Park, Stephen S.

doi:10.1002/lary.20025

Cited by 18 publications

(15 citation statements)

References 8 publications

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“…Prominent soft tissue ingrowth and neovascularization in the implanted construct were shown consistently during the postoperative periods. These features are a positive finding for implants, and would provide stability via incorporation into host tissue, resistance against migration and extrusion, and maintenance of the immune response . These results were similar with the study by Kim et al Additionally, positively stained cells for macrophages were visible around the adjacent tissues of the PCL implant at postoperative 8 weeks.…”

Section: Discussionsupporting

confidence: 90%

“…The role of implants in aesthetic and reconstructive surgery of the nose has evolved considerably during the past several decades . Autologous cartilage is considered to be ideal for all implant types in nasal surgery but is limited by the finite amount of available tissue.…”

Section: Introductionmentioning

confidence: 99%

“…Silicone, supramid mesh, Proplast (Vitek, Houston, TX), Mersiline (Ethicon Inc., Somerville, NJ), hydroxyapatite, and high‐density porous polyethylene are now available and have been used for nasal reconstruction with variable success rates. However, despite advances in alloplastic materials, they are still associated with increased rates of extrusion and infection, as well as with poor biocompatibility …”

Section: Introductionmentioning

confidence: 99%

“…The role of implants in aesthetic and reconstructive surgery of the nose has evolved considerably during the past several decades. 1 Autologous cartilage is considered to be ideal for all implant types in nasal surgery but is limited by the finite amount of available tissue. Additionally, the acquisition of cartilage is associated with various morbidity or mortality, and its use is associated with an unpredictable degree of absorption and inflammation.…”

Section: Introductionmentioning

confidence: 99%

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New application of three‐dimensional printing biomaterial in nasal reconstruction

et al. 2017

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

New application of three‐dimensional printing biomaterial in nasal reconstruction

et al. 2017

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“…4 Tissue engineering or coating with biological material is a promising way to improve PPE performance in vivo. 23 The combination of proangiogenic growth factor containing Matrigel and solid scaffold biomaterial consisting of poly-D,L-lacticco-glycolic acid (PLGA) has proven effective in accelerating vascularization without loss of biocompatibility. 13 Matrigel is available in distinct compositions depending on growth factor content.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of porous polyethylene implants tissue‐engineered by extracellular matrix and VEGF

Strieth¹,

Weger²,

Bartesch³

et al. 2009

J Biomedical Materials Res

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Rapid ingrowth of blood vessels and low inflammatory response are considered major prerequisites for successful implantation of biomaterials in reconstructive surgery. Aim of the present study was to evaluate whether tissue-engineered porous polyethylene (PPE) implants providing extracellular matrix components (ECM) and vascular endothelial growth factor (VEGF) in vivo improve microvascular ingrowth and mechanical integration with regard to initial inflammatory responses. PPE implants (3 x 3 x 0.1 mm(3), pore size approximately 100-200 microm) were tissue-engineered by incorporation of ECM components (GFR-Matrigel) adding recombinant murine VEGF (1 microg/mL) and grafted into dorsal skinfold chamber preparations of C57BL/6 mice. Control animals received uncoated implants or implants coated with ECM components alone (n = 6 per group). Using in vivo fluorescence microscopy angiogenic activity and inflammatory leukocyte-endothelial cell interactions were analyzed for 2weeks. Finally, mechanical integration was quantified by measurement of dynamic desintegration strengths at the host-implant border. Functional vessel density, red blood cell velocity, and vessel diameters increased continuously in all groups indicating that rapid microvascular integration of PPE occurred even without incorporation of ECM or VEGF. However, a transient initial inflammatory response with increased leukocyte-endothelial cell adherence on day 7 in uncoated control implants was efficiently reduced by incorporation of ECM and VEGF. Measurement of dynamic breaking strengths revealed no significant differences between the groups although there was a tendency to improved mechanical integration in tissue-engineered implants. Therefore, novel tissue- engineered constructs of PPE implants providing ECM and VEGF in high local concentrations can increase biocompatibility especially under unfavorable conditions for implantation.

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Adhesion and integration of tissue engineered cartilage to porous polyethylene for composite ear reconstruction

et al. 2014

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The objective of this study was to assess the ability of tissue engineered cartilage to adhere to and integrate with porous polyethylene (PPE) in vivo and to evaluate the biomechanical integrity of the bond formed at the interface. Porcine auricular, articular, and costal chondrocytes were suspended in fibrin gel polymer and placed between discs of PPE to form tri-layer constructs. Controls consisted of fibroblasts suspended in gel or gel alone between the discs. Constructs were implanted into nude mice for 6, 12, and 18 weeks. Upon harvest, specimens were evaluated for neocartilage formation and integration into the PPE, using histological, dimensional (mass, thickness, diameter), and biomechanical (adhesion strength, interfacial stiffness, failure energy and failure strain) analyses. Neotissue was formed in all experimental constructs, consisting mostly of neocartilage integrating with discs of PPE. Control samples contained only fibrous tissue. Biomechanical analyses demonstrated that adhesion strength, interfacial stiffness, and failure energy were all significantly higher in the chondrocyte-seeded samples than in fibroblast-seeded controls, with the exception of costal constructs at 12 weeks, which were not significantly greater than controls. In general, failure strains did not vary between groups. In conclusion, porous polyethylene supported the growth of neocartilage that formed mechanically functional bonds with the PPE.

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Subcutaneous implants coated with tissue‐engineered cartilage

Cited by 18 publications

References 8 publications

New application of three‐dimensional printing biomaterial in nasal reconstruction

New application of three‐dimensional printing biomaterial in nasal reconstruction

Biocompatibility of porous polyethylene implants tissue‐engineered by extracellular matrix and VEGF

Adhesion and integration of tissue engineered cartilage to porous polyethylene for composite ear reconstruction

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