Subclassifying Atypical Squamous Cells in Thin-Prep Cervical Cytology Correlates with Detection of High-Risk Human Papillomavirus DNA

Crum, Christopher P.; Genest, David R.; Krane, Jeffrey F.; Hogan, Christine; Sun, Deqin; Bellerose, Bronya; Kostopoulou, Evanthia; Lee, Kenneth R.

doi:10.1093/ajcp/112.3.384

Cited by 45 publications

(34 citation statements)

References 4 publications

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“…Other investigators have shown that interobserver agreement for the diagnosis of WNL/ BCC and SIL was better than interobserver agreement for the diagnosis of ASCUS. 16 Our findings also support the finding that smears with diagnoses of HSIL and WNL/BCC demonstrated excellent interobserver reproducibility. Conversely, smears with a diagnosis of ASCUS had poor interobserver agreement.…”

Section: Discussionsupporting

confidence: 86%

“…In a recent study, 3 pathologists and 1 senior cytotechnologist reviewed 144 TP slides previously diagnosed as WNL, ASCUS, and SIL. 16 Those authors reported that the interobserver reproducibility for slides with a diagnosis of WNL or SIL was good; however, the reproducibility for a diagnosis of ASCUS was poor. In the current study, the interobserver agreement among 19 cytotechnologists from 3 different laboratories was good, with a mean correlation coefficient of 0.842.…”

Section: Discussionmentioning

confidence: 99%

“…A recent study reported that interobserver agreement for diagnoses of within normal limits (WNL) or SIL was good but was poor for ASCUS using TP. 16 To our best knowledge, there is no study comparing interobserver variability between TP and the conventional method of collecting and preparing cervical smears. Therefore, the purpose of this study was to assess interobserver variability in classifying squamous lesions between the TP method and conventional methods among individual observers and laboratories with different experiences in TP gynecologic cytology.…”

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confidence: 99%

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Interobserver variability

Chhieng

Talley

Roberson

et al. 2002

Cancer

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Interobserver variability

Chhieng

Talley

Roberson

et al. 2002

Cancer

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“…We previously reported an HPV-DNA transcript detection method using cytologic specimens and reverse transcriptase-nested polymerase chain reactions (PCR) (Fujii et al, 1995), and a method for detecting multiplex HPV infection using PCR single-stranded DNA-conformational polymorphism analysis (Nakagawa et al, 2002). We applied these methods to the fluidbased specimens and then performed direct sequencing of the PCR products.HPV testing with PCR using fluid-based specimens, in conjunction with cytologic and biopsy follow-up, has been reported to be useful for estimating the significance of atypical squamous cells of undetermined significance (Crum et al, 1999), and the concordance rate of HC II and PCR has been reported to be approximately 90% for fluid-based specimens (Peyton et al, 1998). HPV screening with HC II and HPV-typing analysis can be performed using residual specimens without the need to collect a second specimen from the patient; this collection system is thus of great advantage to both patients and clinicians.…”

mentioning

confidence: 99%

“…HPV testing with PCR using fluid-based specimens, in conjunction with cytologic and biopsy follow-up, has been reported to be useful for estimating the significance of atypical squamous cells of undetermined significance (Crum et al, 1999), and the concordance rate of HC II and PCR has been reported to be approximately 90% for fluid-based specimens (Peyton et al, 1998). HPV screening with HC II and HPV-typing analysis can be performed using residual specimens without the need to collect a second specimen from the patient; this collection system is thus of great advantage to both patients and clinicians.…”

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confidence: 99%

Papanicolaou tests and molecular analyses using new fluid-based specimen collection technology in 3000 Japanese women

et al. 2003

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A fluid-based Papanicolaou test has been established to improve sample collection and preparation. This study was the first largescale investigation in Japan to examine the feasibility of using fluid-based Papanicolaou specimens to detect human papillomavirus (HPV) using Hybrid Capture II and polymerase chain reaction (PCR). Three thousand patients who visited Keio University Hospital between October 2000 and February 2001 were enrolled in the study. The results of the fluid-based Papanicolaou tests corresponded well with those of conventional Papanicolaou smears (96.8% concordance). The sensitivities of cervical neoplasia detection using the fluid-based Papanicolaou test (73.9%) and Hybrid Capture II (76.3%, P ¼ 0.55) were not significantly different. Among the cervical intraepithelial neoplasia 3 and squamous cell carcinoma specimens, HPV 16 and HPV 52 were predominantly detected using the PCR method. Although some DNA samples extracted from the fluid-based specimens were degradaded, PCR and direct sequencing could be performed without difficulty even after 1 year of specimen storage. We conclude that fluid-based Papanicolaou specimens can be applied to investigate HPV infection. The fluid-based Papanicolaou specimen collection method is widely used for primary cervical cancer screening (Awen et al, 1994;Roberts et al, 1997;Bolick and Hellman, 1998), largely because of the improvement in specimen quality, the advantage of increased sensitivity, and a reduction in the false negative rate for squamous intraepithelial lesions (Linder and Zahniser, 1997;Park et al, 2001), compared to the conventional Papanicolaou smear method. Fluid-based specimens can be stored at ambient temperature for a longer period; therefore, this collection system offers the advantage that once the specimen has been collected, there is no need to collect additional specimens from the patient to perform a second Papanicolaou test or conduct further investigations. Studies evaluating the clinical utility of Hybrid Capture II (HC II) human papillomavirus (HPV)-DNA testing have also been performed (Clavel et al, 1998;Lin CT et al, 2000a;Yamazaki et al, 2001;Castle et al, 2002). Genital HPV has been reported to be related to cervical cancer carcinogenesis and some types of HPV; HPV 16, for example, is associated with a high risk of cervical neoplasia (Josefsson et al, 2000;Woodman et al, 2001). HC II is a HPV detection test designed to detect 18 types of HPV using microtitre plates and is an appropriate method for HPV screening. We performed the HC II test using fluid-based specimens from patients in whom biopsy studies were also performed to determine whether this method is appropriate for detecting cervical neoplasia in Japan. We also performed HPV typing using fluid-based specimens. We previously reported an HPV-DNA transcript detection method using cytologic specimens and reverse transcriptase-nested polymerase chain reactions (PCR) (Fujii et al, 1995), and a method for detecting multiplex HPV infection using PCR single-stranded DNA-conform...

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