Interobserver variability

Chhieng, David C.; Talley, Lynya; Roberson, Janie; Gatscha, Rose Marie; Jhala, Nirag; Elgert, Paul

doi:10.1002/cncr.10477

Cited by 22 publications

(8 citation statements)

References 23 publications

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“…14 Moreover, it has been demonstrated that training of the readers does not necessarily affect the interobserver reproducibility of liquid-based cervical cytology. 15 A similar finding also might reasonably be likely for anal cytology.…”

Section: Discussionmentioning

confidence: 56%

“…Indeed, the morphologic aspects of the cellular modifications caused by HPV infection are very similar in the squamous epithelium of the uterine cervix and the anal canal 14 . Moreover, it has been demonstrated that training of the readers does not necessarily affect the interobserver reproducibility of liquid‐based cervical cytology 15 . A similar finding also might reasonably be likely for anal cytology.…”

Section: Discussionmentioning

confidence: 84%

“…8 The observation that reproducibility is higher for NILM and L-SIL and poor for ASC-US has also been made for cervical cytology. [15][16][17][18] These findings highlight that the borderline category ASC-US represents a source of poor reproducibility in both anal and cervical cytology. Biomarkers might be useful to improve the reproducibility of the most challenging reporting categories.…”

Section: Discussionmentioning

confidence: 92%

“…The interobserver reproducibility of liquid-based cervicovaginal cytology is only moderate or good. 15,21 In the Bethesda Interobserver Reproducibility Study-2 (BIRST-2), the mean agreement on the Pap test results between participants (mainly cytotechnologists and pathologists) and the reference panel interpretation was 62.8%, with variability among the different diagnostic categories. 18 However, it must be specified that, in the BIRST-2 study, participants were provided with single, web-based images for interpretation.…”

Section: Consensusmentioning

confidence: 99%

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Interobserver agreement in the interpretation of anal cytology

Benevolo,

Rollo,

Latini

et al. 2024

Cancer Cytopathology

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BackgroundAnal cytology represents a tool for anal cancer screening in high‐risk populations. In addition to accuracy, the reproducibility of the interpretation is of key importance. The authors evaluated the agreement of anal cytologic interpretation between two cytopathologists.MethodsLiquid‐based cytologic slides from human immunodeficiency virus (HIV)‐negative men who have sex with men (MSM) were evaluated by two readers with at least 10 years of expertise in cervical cytology. Cases with a discordant interpretation were reviewed, and a consensus was reached. Human papillomavirus (HPV) genotyping was performed using a proprietary HPV genotyping test. Unweighted and weighted Cohen kappa and 95% confidence interval (CI) values were calculated.ResultsOverall, 713 slides that were adequate for interpretation were evaluated (MSM: median age, 33 years). An HPV test was performed on 620 samples (87.0%). Considering a dichotomous interpretation (negative for intraepithelial lesion or malignancy vs. atypical squamous cells of undetermined significance or worse), the crude agreement between the two readers was 93.3% (kappa = 0.82; 95% CI, 0.77–0.87). Once a consensus for discordant cases was reached, the best agreement was found for the negative for intraepithelial lesion or malignancy category (511 of 528 samples; 96.8%), whereas the atypical squamous cells of undetermined significance category showed the lowest agreement (90 of 117 samples, 76.9%). Considering the individual cytologic categories, overall agreement was 92.1% (kappa = 0.85; 95% CI, 0.81–0.89). The discordant interpretations were not associated with high‐risk HPV infection, HPV16 infection, or MSM age.ConclusionsThe results indicating excellent interobserver agreement in this study substantiate the use of anal cytology in the setting of human immunodeficiency virus‐negative MSM.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 92%

Section: Consensusmentioning

confidence: 99%

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Interobserver agreement in the interpretation of anal cytology

Benevolo,

Rollo,

Latini

et al. 2024

Cancer Cytopathology

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“…This interpretation, coupled with other sources of variation (antigen selection and antibody isotype), causes a lack of consistency in the results 4,5. We are not aware of any studies examining inter-microscopist variability in the reading of IFA slides for the diagnosis of any infectious disease or examining the consequences of IFA slide storage on results 6. We therefore assessed inter-observer and intra-observer variability in the reading of scrub typhus and murine typhus immunoglobulin M (IgM) antibody IFAs plus the consequences on the results of keeping the prepared slides for 14 days at 4°C.…”

Section: Introductionmentioning

confidence: 99%

Inter- and Intra-Operator Variability in the Reading of Indirect Immunofluorescence Assays for the Serological Diagnosis of Scrub Typhus and Murine Typhus

Phetsouvanh¹,

Thojaikong²,

Phoumin³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Inter- and intra-observer variation was examined among six microscopists who read 50 scrub typhus (ST) and murine typhus (MT) indirect immunofluorescence assay (IFA) immunoglobulin M (IgM) slides. Inter-observer agreement was moderate (κ = 0.45) for MT and fair (κ = 0.32) for ST, and was significantly correlated with experience (P = 0.03 and P = 0.004, respectively); κ-scores for intra-observer agreement between morning and afternoon readings (range = 0.35–0.86) were not correlated between years of experience for ST and MT IFAs (Spearman's ρ = 0.31, P = 0.54 and P = 0.14, respectively; P = 0.78). Storage at 4°C for 2 days showed a change from positive to negative in 20–32% of slides. Although the titers did not dramatically change after 14 days of storage, the final interpretation (positive to negative) did change in 36–50% of samples, and it, therefore, recommended that slides should be read as soon as possible after processing.

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Results of the implementation of liquid-based cytology-SurePath in the Ontario screening program

Colgan

McLachlin

Cotterchio

et al. 2004

Cancer

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BACKGROUND The objective of the current study was to evaluate the adequacy and detection rates of SurePath after its implementation in Ontario. METHODS The detection and adequacy rates of the SurePath liquid‐based cytology system (SP‐LBC) were calculated for manually reviewed slides of the year 2002. The adequacy and detection rates from this study group were compared with a historical conventional smear (CS) group from the same laboratories during the same period of the previous year. RESULTS The SP‐LBC study group consisted of 352,680 specimens with cytodiagnoses and the CS group included 378,990 specimens. The unsatisfactory rate for SP‐LBC (0.24%) was less than that of the CS group (0.58%). The detection rate of atypical squamous cells (ASC+) by the SP‐LBC group (4.69%) was greater than that of the CS group (3.81%), as was the detection rate of low‐grade squamous intraepithelial lesions (LSIL+; 2.13% vs. 1.50% in the CS group). There was only a trend toward increased detection of high‐grade squamous intraepithelial lesions (HSIL+) in the SP‐LBC group (0.34%) relative to the CS group (0.31%), because the detection rate for carcinoma by SP‐LBC declined. CONCLUSIONS The implementation of SP‐LBC has been followed by better specimen adequacy and detection rates for ASC+, LSIL+, and a trend of increased detection of HSIL+ relative to CS practice. To determine sensitivity rates, a histopathologic database for cervical carcinoma and precancer needs to be established. Cancer (Cancer Cytopathol) 2005. © 2004 American Cancer Society.

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Interobserver variability

Cited by 22 publications

References 23 publications

Interobserver agreement in the interpretation of anal cytology

Interobserver agreement in the interpretation of anal cytology

Inter- and Intra-Operator Variability in the Reading of Indirect Immunofluorescence Assays for the Serological Diagnosis of Scrub Typhus and Murine Typhus

Results of the implementation of liquid-based cytology-SurePath in the Ontario screening program

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