Subclasses of porcine immunoglobulins G. Production of subclass specific antisera with S‐sulfonated gamma chains

Abstract

Using antisera made against porcine S‐sulfonated heavy chains two subclasses of porcine IgG could be distinguished on the basis of antigenic determinants located on the Fc fragment. The antisera prepared against the porcine gamma chains reacted with other mammalian IgG proteins, but not with avian, amphibian or fish immunoglobulins. Antigenic determinants responsible for cross‐reactivity are further localized on the Fc′ fragment of the porcine IgG molecule. Comparable antisera, specific for porcine light chains, did not cross‐react with IgG from any of the other species tested. Porcine IgG and F(ab′)2 fragment reacted with both the anti‐heavy and anti‐light chain sera, while the Fab fragment reacted only with the anti‐light chain sera. Important antigenic determinants are thus located on the light chains and the Fc portion of the heavy chain of the porcine IgG molecule.

Surface Markers of Porcine Lymphocytes and Distribution in Various Lymphoid Organs

“…Receptor-Bearing Cells Porcine IgG, obtained by elution from DEAEcellulose columns [22], was conjugated to fluores cein isothiocyanate and aggregated at 63 °C for 20 min [8], In this way, 75%> of IgG were aggre gated. The final preparation was adjusted to 50 mg/ml and stored at 4 °C until use.…”

“…Immunoglobulin fractions of anti-« serum, prepared in rabbits with resolubilized «/-chains [22], was conjugated with peroxidase by the meth od of Kawoi and Nakane [17]. Polyvalent peroxi dase anti-Ig was purchased from the Pasteur Insti tute (Paris).…”

Synthesis of Immunoglobulins IgG, IgM and IgA during the Ontogeny of Foetal Pigs

“…Rabbits were immunized intramuscularly with 1 mg doses of resolubilized y-and ",-chains or purified IgA in complete Freund's adjuvant at three fortnightly intervals (Metzger and Houdayer 1972). They were exsanguinated one week after the last immunization.…”

Revisiting the Pig IGHC Gene Locus in Different Breeds Uncovers Nine Distinct IGHG Genes

“…In cattle and other ruminants, IgG1 is selectively transported by the alveolar epithelial cells in the mammary gland and other sites and can lead to levels exceeding 100 mg/ml IgG1 in colostrum compared with ,5 mg/ml IgG2 (13). Also, the response profile to different antigenic stimuli differs among subclasses (14)(15)(16)(17)(18). During early HIV-1 infection, functional Ab responses decline drastically with HIV disease progression, which is correlated with a selective waning IgG3 concentration (19)(20)(21).…”