Surface Markers of Porcine Lymphocytes and Distribution in Various Lymphoid Organs

-Besides their breeding value, swine are increasingly used as biomedical models. As reported in three international swine clusters of differentiation (CD) workshops and in the animal homologue section of the last workshop for the determination of human leukocyte differentiation antigens (HLDA 8), characterisation of leukocyte surface antigens by monoclonal antibodies and other molecular studies have determined the cell lineages and blood leukocyte subsets implicated in the immune response, including cell adhesion molecules involved in cell trafficking. This review focusses on the current state of knowledge of porcine leukocyte differentiation and major histocompatibility complex (SLA) molecules. Examples of porcine particularities such as the double-positive T lymphocytes with the phenotype CD4 + CD8 low and CD4 − CD8 low T cell subsets and the persistence of SLA class II after T-lymphocyte activation are illustrated, as well as the shared characteristics of the Artiodactyla group, such as the high proportion of TcR (T cell receptor) T cells in blood and other lymphoid tissues. Furthermore, discrepancies between swine and humans, such as CD16 expression on dendritic cells and CD11b (wCD11R1) tissue distribution are outlined. The rapidly growing information should facilitate manipulation of the swine immune system towards improving disease control, and open new avenues for biomedical research using the pig as a model. cluster of differentiation (CD) / monoclonal antibody / immune system / histocompatibility system / pig

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“…In swine, as well as in ruminants, there is a high proportion of null cells (≥30% in the blood of piglets [134,180] …”

Section: Markers Of Tcr-t Cells and 'Null Cells'mentioning

confidence: 99%

“…This population is characterised by expression of the BcR representing a stable (i.e. not elutable) membrane immunoglobulin [180] that can be recognised by antibodies against the Ig light chain [193]. Extensive work on the antibody repertoire development was done and reviewed by the Butler laboratory [42].…”

Section: Differentiation Antigens Of B Cellsmentioning

confidence: 99%

Membrane markers of the immune cells in swine: an update

Piriou-Guzylack¹,

2008

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“…In contrast to L14, sublines L52 and L23 were actively secreting immunoglobulin molecules in relatively high amounts (1.2 to 1.8 Fg/ml, Table IV). Follow- In conclusion, the presence of MSA, antigen without menibrane-bound immunoglobulins on the cells of L35 and L4.5 suggest the T-lymphocyte origin of the cells (Salmon, 1979).…”

mentioning

confidence: 76%

“…Membrane immunoglobulins were detected by indirect immunofluorescence (Salmon, 1979) in cell suspension using the available MAbs specific to heavy chains of p, (28.4.1 MAb kind gift of Dr. Bianchi, Lelystad, The Netherlands, and K513D MAb, kind gift of Dr. Steven, University of Bristol, UK), gamma (23.7.1. MAb from Dr. Bianchi), alpha (27.9.1.…”

Section: Morphology and Cytoenzyrnology Cells Were Classified Aftermentioning

confidence: 99%

Histocompatible miniature boar model: Selection of transformed cell lines of b and t lineages producing retrovirus

Kaeffer¹,

Bottreau²,

Thanh³

et al. 1990

Intl Journal of Cancer

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A lymphoblastoid cell line (B1) was isolated in culture following a brief exposure to 5-azacytidine from peripheral-blood mononuclear cells of a boar previously injected with cells (Shimozuma) producing porcine retrovirus (Tsukuba-1) and suffering a severe non-neoplastic syndrome at autopsy. B1 cell line and 5 of its sublines were propagated for more than 100 generations, retaining doubling times comprised between 16.8 and 27.5 hr and growing readily in agarose or agar (plating efficiency: 5 to 50%). Karyotype analyses showed that 4 sublines were nearly diploid, except for cells of L14, which displayed a monosomy affecting chromosome 18 pair. Two sublines (L35 and L45) were considered as being of T-cell lineage, since MSA, antigen was observed on the surface of approximately 30% of cells. Three sublines (L23, L14 and L52) were considered of B-cell lineage, since membrane immunoglobulins were observed on the cell surface. In addition, sublines L23 and L52 were actively secreting immunoglobulin of mu isotype. Retrovirus particles were evidenced in gradient-purified preparation of 200-fold-concentrated cell culture supernatants of the B1 cell line, L14, L35 and L52 sublines, using both a reverse transcriptase activity assay and electron microscopic observation. These cell lines can be used to select for porcine retrovirus variants with transforming potential for lymphocytes of B and T lineages.

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“…Ces espèces représentent ainsi un modèle d'étude du développement de la réponse immunitaire chez le foetus en l'absence d'anticorps (Kim et al, 1966 ;Sterzl et Silverstein, 1967) (Solomon, 19711. 2) Lymphocytopoiëse. &horbar; D'après Solomon (1971) (Chapman et al, 1974 (Salmon, 1982 (Salmon, 1979), le sang et le thymus présentent dès le 80 e jour de gestation un profil adulte, l'intestin et le ganglion mésentérique seulement à la naissance ; la rate est le seul organe qui à la naissance ne présente pas le type adulte ; cependant ce retard est comblé 4 jours après (Salmon, 1982).…”

unclassified