Subcellular distribution of Cd and Zn and MT mRNA expression in the hepatopancreas of Sinopotamon henanense after single and co-exposure to Cd and Zn

Li, Yingjun; Wu, Hao; Wei, Xiang; He, Yongmei; Li, Baozhen; Li, Yongquan; Jing, Weixin; Wang, Lan

doi:10.1016/j.cbpc.2014.09.006

Cited by 10 publications

(3 citation statements)

References 56 publications

(88 reference statements)

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“…Additionally, the main role of the metal ion detoxification processes is played by the protein metallothionein (MT), a low-molecular-weight, cysteine-rich, and metal-binding protein 28 , 29 . Importantly, it has been shown that metals play a major role in regulating MT mRNA expression at the transcription level 28 , 30 and that inducing MT is thought to be essential for reducing Cd toxicity, such as oxidative damage 31 .…”

Section: Introductionmentioning

confidence: 99%

Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress

Yang,

Li,

Zhu

et al. 2023

Sci Rep

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Because China produces the most crayfish in the world, safe solutions must be improved to mitigate the risks of ongoing heavy metal stressors accumulation. This study aimed to use Saccharomyces cerevisiae as a bioremediation agent to counteract the harmful effect of cadmium (Cd) on crayfish (Procambarus clarkia). Our study used three concentrations of S. cerevisiae on crayfish feed to assess their Cd toxicity remediation effect by measuring total antioxidant capacity (TAC) and the biomarkers related to oxidative stress like malondialdehyde (MDA), protein carbonyl derivates (PCO), and DNA–protein crosslink (DPC). A graphite furnace atomic absorption spectroscopy device was used to determine Cd contents in crayfish. Furthermore, the mRNA expression levels of lysozyme (LSZ), metallothionein (MT), and prophenoloxidase (proPO) were evaluated before and following the addition of S. cerevisiae. The results indicated that S. cerevisae at 5% supplemented in fundamental feed exhibited the best removal effect, and Cd removal rates at days 4th, 8th, 12th, and 21st were 12, 19, 29.7, and 66.45%, respectively, which were significantly higher than the basal diet of crayfish. The addition of S. cerevisiae increased TAC levels. On the other hand, it decreased MDA, PCO, and DPC, which had risen due to Cd exposure. Furthermore, it increased the expression of proPO, which was reduced by Cd exposure, and decreased the expression of LSZ and MT, acting in the opposite direction of Cd exposure alone. These findings demonstrated that feeding S. cerevisiae effectively reduces the Cd from crayfish and could be used to develop Cd-free crayfish-based foods.

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Section: Introductionmentioning

confidence: 99%

Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress

Yang,

Li,

Zhu

et al. 2023

Sci Rep

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“…Cadmium also can result in the up‐regulation of cellular protective mechanisms, such as antioxidant defense systems and cellular repair pathways . Cadmium‐induced expression of various proteins of functional importance, such as the superoxide dismutase, glutathione peroxidase, catalase, and metallothionein, can be observed in tissues (i.e., hepatopancreas and gills) of the freshwater crab S. henanense . The present study was focusing on the GSH and metallothionein synthesis because they are important in the defense against Cd stress.…”

Section: Discussionmentioning

confidence: 99%

“…Specific primers of Vtg were designed for quantitative real‐time polymerase chain reaction (qRT‐PCR) from Vtg sequences available from NCBI (KP319023), using Primer Premier 5.0 software (Premier Biosoft International), and listed in Table . The primers of metallothionein, glyceraldehyde 3‐phosphate dehydrogenase (GAPDH), cytochrome C oxidase (COX), isocitrate dehydrogenase (IDH), and beta‐actin (β‐actin ) were according to Li et al and Xuan et al . Expression of messenger RNA (mRNA) for 6 genes in ovaries was determined by qRT‐PCR, using an ABI 7500 Real Time PCR System (Applied Biosystem) and Quantitect SYBR green RT‐PCR Master Mix (Qiagen).…”

Section: Methodsmentioning

confidence: 99%

Cadmium inhibits the vitellogenesis of freshwater crab Sinopotamon henanense

Yang

Dahms‬

et al. 2015

Enviro Toxic and Chemistry

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Cadmium (Cd) may pose risks to freshwater organisms, including crabs that live at the interface of sediments and water column all year round. One of the major changes that occur during oocyte maturation of crabs is the production of vitellin. In the present study, the authors investigated the effects of Cd on oocyte size, vitellin level, and vitellogenin (Vtg) messenger RNA (mRNA) expression in the ovary of the freshwater crab Sinopotamon henanense. The authors studied the impacts of Cd on carbohydrate as well as on the protein metabolism, metallothionein, glutathione (GSH) synthesis, energy-related parameters, and mRNA expression of genes involved in energy metabolism. After Cd treatment, vitellin concentration, Vtg mRNA expression, and oocyte diameter decreased. Less carbohydrate and enhanced protein catabolism were found in the ovary. Adenosine triphosphate to adenosine diphosphate (ATP:ADP) ratios, reduced nicotinamide adenine dinucleotide (NADH) to oxidized nicotinamide adenine dinucleotide (NAD(+) ) ratios, mitochondrial membrane potential, and energy production-related mRNA expressions increased in the ovary after 10 d Cd treatment and decreased after 20 d. Metallothionein and GSH synthesis were up-regulated after 10 d on Cd exposure. Moreover, Cd caused a time-dependent up-regulation of malondialdehyde. The authors' findings show significant effects of Cd on vitellogenesis, which suggests that Cd slows down vitellogenesis in S. henanense because of excessive energy consumption and an activated defense system.

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Cadmium induced oxidative damage and apoptosis in the hepatopancreas of Meretrix meretrix

et al. 2016

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Even trace amounts of cadmium (Cd), a non-essential metal, are known to be toxic to aquatic organisms. Here we investigated the relationship between cadmium ion (Cd(2+)) exposure and oxidative damage and apoptosis in the hepatopancreas of the clam Meretrix meretrix. Clams were exposed to different concentrations of Cd(2+) (0, 1.5, 3, 6 and 12 mg L(-1)) for 5 days. We monitored both antioxidant enzyme activity, including that of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx), and levels of malondialdehyde (MDA), glutathione (GSH) and glutathione disulfide (GSSG). Apoptosis of hepatopancreatic cells was detected by DNA laddering and AO/EB double fluorescent staining. The results show that the rate of apoptotis, MDA levels, and caspase-3 activity, increased with Cd(2+) concentration, whereas GPx activity and the ratio of GSH/GSSG, decreased. SOD and CAT enzyme activity first increased, then decreased, with increasing Cd(2+) concentration; peak activity of these enzymes was recorded in the 3 mg L(-1) Cd(2+)-treatment group. These results show that Cd-induced oxidative damage can both induce, and aggravate, apoptosis in the hepatopancreatic cells of clams, even at Cd(2+) concentrations far below the semi-lethal dose for adult clams. The observed changes in caspase-3 activity enhanced significantly at lower Cd(2+) concentrations, indicating that caspase-3 is a suitable biomarker for heavy metal pollution, especially cadmium pollution, in marine organisms.

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Subcellular distribution of Cd and Zn and MT mRNA expression in the hepatopancreas of Sinopotamon henanense after single and co-exposure to Cd and Zn

Cited by 10 publications

References 56 publications

Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress

Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress

Cadmium inhibits the vitellogenesis of freshwater crab Sinopotamon henanense

Cadmium induced oxidative damage and apoptosis in the hepatopancreas of Meretrix meretrix

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