Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress

Abstract

Because China produces the most crayfish in the world, safe solutions must be improved to mitigate the risks of ongoing heavy metal stressors accumulation. This study aimed to use Saccharomyces cerevisiae as a bioremediation agent to counteract the harmful effect of cadmium (Cd) on crayfish (Procambarus clarkia). Our study used three concentrations of S. cerevisiae on crayfish feed to assess their Cd toxicity remediation effect by measuring total antioxidant capacity (TAC) and the biomarkers related to oxidative stress like malondialdehyde (MDA), protein carbonyl derivates (PCO), and DNA–protein crosslink (DPC). A graphite furnace atomic absorption spectroscopy device was used to determine Cd contents in crayfish. Furthermore, the mRNA expression levels of lysozyme (LSZ), metallothionein (MT), and prophenoloxidase (proPO) were evaluated before and following the addition of S. cerevisiae. The results indicated that S. cerevisae at 5% supplemented in fundamental feed exhibited the best removal effect, and Cd removal rates at days 4th, 8th, 12th, and 21st were 12, 19, 29.7, and 66.45%, respectively, which were significantly higher than the basal diet of crayfish. The addition of S. cerevisiae increased TAC levels. On the other hand, it decreased MDA, PCO, and DPC, which had risen due to Cd exposure. Furthermore, it increased the expression of proPO, which was reduced by Cd exposure, and decreased the expression of LSZ and MT, acting in the opposite direction of Cd exposure alone. These findings demonstrated that feeding S. cerevisiae effectively reduces the Cd from crayfish and could be used to develop Cd-free crayfish-based foods.

Elucidating the Role of Prickly Pear Fruits (Opuntia littoralis) in Mitigation of Cadmium Toxicity in Oreochromis niloticus: Impacts on Haemato-Biochemical and Immunological Responses

“…These observations are consistent withGoda (2008),Sonmez et al (2015), andYang et al (2023), who reported that herbal plants used as a supplemented diet increased the serum protein levels in sh. After cadmium toxicity, all OLFE-forti ed diets effectively reduced all biochemical parameters (lipids, cortisol, protein, glucose, ASAT, creatinine, and ALAT).…”

The Effects of Dietary Saccharomyces cerevisiae Supplementation on Gut Microbiota Composition and Gut Health in Aged Labrador Retrievers

“…As for S. cerevisiae , European seabass fed live S. cerevisiae had higher levels of SOD and GSH-Px in addition to lower MDA levels than fish on a basic diet, which were accompanied by downregulated heat shock protein 70 gene expression and markedly improved hematobiochemical and immune performance [ 32 ]. In addition, the inclusion of dietary S. cerevisiae increased the total antioxidant capacity and suppressed a rise in MDA and protein carbonyl derivatives caused by oxidative damage under cadmium stress in crayfish [ 33 ]. Furthermore, another study assessed the impact of a combination of live S. cerevisiae with yeast postbiotics and selenium-fortified yeast on ewes.…”