Subcellular coordination of plant cell wall synthesis

Hoffmann, Natalie; King, S. B.; Samuels, A. Lacey; McFarlane, Heather E.

doi:10.1016/j.devcel.2021.03.004

Cited by 62 publications

(37 citation statements)

References 138 publications

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“…We identified 248 proteins that were present with 'high' confidence in all three experiments and in at least six out of the eight biological replicates. To further refine this list, we used SUBA (Hooper et al, 2017) to filter for proteins that are predicted to localize to the Golgi apparatus, resulting in 94 candidates (Figure 5E). Comparison of these results with previously published Golgi proteomes (Nikolovski et al, 2012;Parsons et al, 2012;Parsons et al, 2019) revealed that 40% of the proteins identified via NKS1-GFP immunoprecipitation were a subset of these Golgi proteomes (Figure 5E; Table S4).…”

Section: Resultsmentioning

confidence: 99%

“…Different Golgi cisternae are associated with different biochemical functions, i.e., the assembly or modification of certain cell wall components (Zabotina et al, 2021;Hoffmann et al, 2021). To investigate whether NKS1 is associated with certain cisternae, we next crossed the NKS1-GFP or NKS1-RFP fluorescent lines with markers for the cis-Golgi (NAG, Grebe et al, 2003), medial-Golgi (XYLT, Saint-Jore-Dupas et al, 2006) or trans-Golgi (ST, Renna et al, 2005) to generate dual-labelled fluorescent lines.…”

Section: Resultsmentioning

confidence: 99%

“…For example, in seed coat epidermal cells, which synthesize an extraordinary volume of pectic mucilage during their development, Golgi stacks showed swollen margins, many associated vesicles, and a complex trans-Golgi network, while these changes were not observed in mutants lacking a key pectin synthesis gene . Whether these structural changes to the Golgi reflect an active remodeling of the endomembrane system or are a passive consequence of polysaccharide flux through the Golgi remains to be determined (Hoffmann et al, 2021). Notably, in mammalian (HeLa) cells, changes to Golgi protein interactions were correlated with loss of GT function and dramatic changes to Golgi structure (van Galen et al, 2014), implying an important relationship between Golgi structure and function.…”

Section: Discussionmentioning

confidence: 99%

“…To select the candidates the samples were further filtered selecting for those with an average abundance across all experiments ≥100, found with 'high' confidence in all rounds, and present in at least 6 samples, resulting in 248 proteins. We then used SUBA (Hooper et al, 2017) to narrow down potential candidates. AGIs corresponding to the proteins identified above the quality control thresholds described were input to SUBA and filtered for "Location All Predictors" containing the term "golgi".…”

Section: Immunoprecipitation Of Nks1-gfp and Interactorsmentioning

confidence: 99%

“…While pectin synthesis occurs in the Golgi apparatus, there is contradictory data as to whether proteins required for HG synthesis are distributed across different Golgi cisterna (Zhang & Staehelin, 1992;Parsons et al, 2019), or whether HG synthesis proteins act as part of multiprotein complexes (Atmodjo et al, 2011;Harholt et al, 2012;Atmodjo et al, 2013), or a combination of both models (Zabotina et al, 2021;Hoffmann et al, 2021). A better understanding of the pectin synthesis machinery and its interactors is required to appreciate the synthesis of this class of polysaccharides and to open potential for pectin engineering for agricultural improvements.…”

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confidence: 99%

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A DUF1068 protein acts as a pectin biosynthesis scaffold and maintains Golgi morphology and cell adhesion in Arabidopsis

Lathe

McFarlane

Khan

et al. 2021

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Adjacent plant cells are connected by specialized cell wall regions, called middle lamellae, which influence critical agricultural characteristics, including fruit ripening and organ abscission. Middle lamellae are enriched in pectin polysaccharides, specifically homogalacturonan (HG). Here, we identify a plant-specific Arabidopsis DUF1068 protein, called NKS1, that is required for middle lamellae integrity and cell adhesion. NKS1 localises to the Golgi apparatus and loss of the protein results in changes to Golgi structure and function. The nks1 mutants also display HG deficient phenotypes, including reduced seedling growth, changes to cell wall composition, and tissue integrity defects. These phenotypes are identical to those of the HG deficient mutants qua1 and qua2. Notably, NKS1 physically interacts with both QUA1 and QUA2, and genetic interaction analyses reveal that they work in the same pathway. Based on these results we propose that NKS1 works as a scaffold for HG synthesis and that such scaffolding is important to support Golgi function and the organization of the pectin synthesis machinery.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Immunoprecipitation Of Nks1-gfp and Interactorsmentioning

confidence: 99%

mentioning

confidence: 99%

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A DUF1068 protein acts as a pectin biosynthesis scaffold and maintains Golgi morphology and cell adhesion in Arabidopsis

Lathe

McFarlane

Khan

et al. 2021

Preprint

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Combining extracellular matrix proteome and phosphoproteome of chickpea and meta‐analysis reveal novel proteoforms and evolutionary significance of clade‐specific wall‐associated events in plant

Narula,

Sinha,

Choudhary

et al. 2024

Plant Direct

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Extracellular matrix (ECM) plays central roles in cell architecture, innate defense and cell wall integrity (CWI) signaling. During transition to multicellularity, modular domain structures of ECM proteins and proteoforms have evolved due to continuous adaptation across taxonomic clades under different ecological niche. Although this incredible diversity has to some extent been investigated at protein level, extracellular phosphorylation events and molecular evolution of ECM proteoform families remains unexplored. We developed matrisome proteoform atlas in a grain legume, chickpea and performed meta‐analyses of 74 plant matrisomes. MS/MS analysis identified 1,424 proteins and 315 phosphoproteins involved in diverse functions. Cross‐species ECM protein network identified proteoforms associated with CWI maintenance system. Phylogenetic characterization of eighteen matrix protein families highlighted the role of taxon‐specific paralogs and orthologs. Novel information was acquired on gene expansion and loss, co‐divergence, sub functionalization and neofunctionalization during evolution. Modular networks of matrix protein families and hub proteins showed higher diversity across taxonomic clades than among organs. Furthermore, protein families differ in nonsynonymous to synonymous substitution rates. Our study pointed towards the matrix proteoform functionality, sequence divergence variation, interactions between wall remodelers and molecular evolution using a phylogenetic framework. This is the first report on comprehensive matrisome proteoform network illustrating presence of CWI signaling proteins in land plants.

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Glycoside hydrolase family 16—Xyloglucan:xyloglucosyl transferases and their roles in plant cell wall structure and mechanics

Stratilová¹,

Kozmon²,

Stratilová³

et al. 2023

Glycoside Hydrolases

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Subcellular coordination of plant cell wall synthesis

Cited by 62 publications

References 138 publications

A DUF1068 protein acts as a pectin biosynthesis scaffold and maintains Golgi morphology and cell adhesion in Arabidopsis

A DUF1068 protein acts as a pectin biosynthesis scaffold and maintains Golgi morphology and cell adhesion in Arabidopsis

Combining extracellular matrix proteome and phosphoproteome of chickpea and meta‐analysis reveal novel proteoforms and evolutionary significance of clade‐specific wall‐associated events in plant

Glycoside hydrolase family 16—Xyloglucan:xyloglucosyl transferases and their roles in plant cell wall structure and mechanics

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