“…The importance of these types of cross-linking for cell adhesion has been supported by the characterisation of celladhesion defective mutants. For instance, in Arabidopsis, several mutations in genes involved in HG synthesis (Bouton et al, 2002;Du et al, 2020;Lathe et al, 2021;Mouille et al, 2007;Temple et al, 2021), remodelling (Francis et al, 2006;Lionetti et al, 2015) or degradation (Ogawa et al, 2009;Rhee et al, 2003;Rui et al, 2019) harbour cell-adhesion phenotypes (loss of cell adhesion or incapacity of cell separation). Furthermore, selectively digesting the cell wall with pectin degrading enzymes, such as polygalacturonases, and even chelating the cell wall calcium with chelators, such as EDTA, can also lead to cell separation (Letham, 1960;McCartney & Knox, 2002;Ng et al, 2000;Zhang et al, 2000).…”