Subacute toxicopathological studies of methotrexate in Wistar rats

Patel, Niti N.; Ghodasara, D. J.; Pandey, Sunanda; Ghodasara, Priya D.; Khorajiya, J.H.; Joshi, B. P.; Dave, C. J.

doi:10.14202/vetworld.2014.489-495

Cited by 35 publications

(29 citation statements)

References 14 publications

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“…This result is in agreement with the study of Aouacheri et al [23]. On the contrary [9], were obtained a weight loss in MTX-treated rats, which might be a reflection of direct toxicity, reduced feed and water intake and stressogenic activity.…”

Section: Parameterssupporting

confidence: 93%

“…Probably, the change in erythrocyte count can be attributed to ulcers in the digestive tract resulting in gradual blood loss which supports the findings of Patel et al [9], and eventually, leading to anaemia [26]. In the other hand, a perturbation in percentage of differential leukocyte cells was appeared in high-doses MTX-induced rats, as evidenced by the decrease in the neutrophils count was observed with an increase in lymphocytes count.…”

Section: Parameterssupporting

confidence: 81%

“…However, the use of high-dose methotrexate (as in leukaemia) or prolonged use may result in hepatotoxicity that may lead to progressive fibrosis and cirrhosis. Methotrexate seems to be hepatotoxic, nephrotoxic and toxic to respiratory and reproductive system at very low doses for continuous therapy [9].…”

Section: Introductionmentioning

confidence: 99%

“…Toxicity studies with methotrexate highlight the role of oxidative stress in causing toxicity on the liver, kidney, heart and other organs [2]. The most serious side effect of MTX therapy is the hepatic toxicity [11], illustrated by elevated liver enzymes [9]. It is reported that mild hepatitis, cholestasis, fatty changes, fibrosis and cirrhosis, in patients receiving MTX for malignant disorders [2].…”

Section: Introductionmentioning

confidence: 99%

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The Investigation of the Oxidative Stress-Related Parameters in High Doses Methotrexate-Induced Albino Wistar Rats

Saka¹,

Aouacheri²

2017

J Bioequiv Availab

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Section: Parameterssupporting

confidence: 93%

Section: Parameterssupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Investigation of the Oxidative Stress-Related Parameters in High Doses Methotrexate-Induced Albino Wistar Rats

Saka¹,

Aouacheri²

2017

J Bioequiv Availab

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“…Patel et al noticed a highly significant increase in the level of ALP in MTX-treated rats which is consistent with our findings. Such significant increase in ALP may be attributed to damage of the body tissues by MTX resulting in liberation of ALP in serum [54] .…”

Section: Discussionmentioning

confidence: 99%

The Protective Role of Simvastatin on Methotrexate-Induced Bone Injury in Adult Albino Rat

Elsaid

Sadek

2017

Egyptian Journal of Histology

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Background: Methotrexate (MTX) is a folic acid antagonist and chemotherapeutic agent widely used in cancer treatment. It is used as first line therapy in treatment of rheumatoid arthritis (RA). MTX was known to cause bone defects in the form of reduced mineral density (BMD) and bone fractures. Simvastatin (SIM) is widely used for cardiovascular diseases. Aim of Work: To investigate the possible protective role of SIM on MTX induced bone injury in rats. Material and Methods: Forty adult male albino rats were divided into four groups; Control group, SIM-treated group, MTX group and MTX + SIM group. MTX was given by subcutaneous injection as 0.65 mg/kg/day for two separate 5 days. SIM was administered orally as 25 mg/kg/day one month prior to and during the MTX course. At the end of the experiment, blood samples were collected for levels of osteocalcin (OSC) and alkaline phosphatase (ALP). All rats were sacrificed and specimens from their femurs were examined. Results: examination of MTX group showed marked thinning of the periosteum, and widening of bone marrow spaces. There was an apparent decrease in the number of osteocytes, together with an apparent increase in the number of osteoclasts. There was a significant decrease in the serum level of OSC together with a highly significant increase in the serum level of ALP in the MTX group as compared to the control group. On administration of SIM with MTX, there was marked improvement in most of the histological and serological parameters. Conclusion: SIM could be used as a protective measure for MTX-induced bone defects.

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RETRACTED ARTICLE: Impact of gervital against histopathological, ultrastructural, and biochemical alterations caused by methotrexate or azathioprine in albino rat testis

Abdul-Hamid

Abdel-Reheim

Hegazy

et al. 2022

Environ Sci Pollut Res

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Methotrexate (MTX) and azathioprine (AZA) are chemotherapeutic, immunosuppressive, cytotoxic drugs with reported adverse effects, including oxidative damage to testis. This study aims to evaluate the potential effect of grape seed extract (GSE; gervital) to prevent testicular damage caused by MTX and AZA. Male albino rats were separated into six groups: group I, normal control group; group II, GSE (150 mg/kg/day); group III, MTX (8 mg/kg/week); group IV, AZA (15 mg/kg/day); group V, GSE (150 mg/kg/day) + MTX (8 mg/kg/week); group VI, GSE (150 mg/kg/day) + AZA (15 mg/kg/day). All rats were sacrificed, blood samples were obtained for testosterone analysis, and testis was removed for histological and ultrastructural studies and oxidation measurements. A reduction in relative body and testis weight, along with a significant decrease in testosterone levels, was observed. Histopathological and ultrastructural alterations induced by MTX or AZA included reduced spermatozoa, sloughing, marked reduction of spermatogenic cells, and pyknosis of some nuclei. Significant oxidative stress manifested as reduced glutathione (GSH) levels and catalase (CAT) and superoxide dismutase (SOD) activities, as well as increased malondialdehyde (MDA) levels. GSE administration showed an ameliorative effect on testosterone levels and histopathological and ultrastructural changes. GSE treatment also suppressed the increases in MDA levels and the decreases in GSH levels and CAT and SOD activities. In conclusion, these findings confirm that GSE is an effective antioxidant that protects testis from histopathological and ultrastructural damage induced by MTX and AZA. Therefore, GSE is a promising candidate for future use to minimize and alleviate MTX and AZA risks. Graphical abstract

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Subacute toxicopathological studies of methotrexate in Wistar rats

Cited by 35 publications

References 14 publications

The Investigation of the Oxidative Stress-Related Parameters in High Doses Methotrexate-Induced Albino Wistar Rats

The Investigation of the Oxidative Stress-Related Parameters in High Doses Methotrexate-Induced Albino Wistar Rats

The Protective Role of Simvastatin on Methotrexate-Induced Bone Injury in Adult Albino Rat

RETRACTED ARTICLE: Impact of gervital against histopathological, ultrastructural, and biochemical alterations caused by methotrexate or azathioprine in albino rat testis

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